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Solid-state UV laser-induced fluorescence detection in capillary electrophoresis.
Electrophoresis. 2000 Jun; 21(10):2062-6.E

Abstract

Two solid-state UV lasers were applied to the laser-induced fluorescence (LIF) detection of various groups of compounds after separation by capillary electrophoresis. These lasers are thermoelectric-cooled, highly compact, and inexpensive. Such lasers provide few mW of quasi-continuous wave (CW) power which are sufficient and stable for LIF detection. Native fluorescence detection of tryptophan-containing proteins and peptides and related indoles was achieved at the nM level with the laser operating at 266 nm. Detection of fluorescamine-labeled amino acids and peptides was also possible at the nM level with the laser operating at 355 nm. Amino acids at a concentration as low as 10 ng/mL could be labeled with fluorescamine. Solid-state UV-LIF detection of the tryptic digest of cytochrome c after fluorescamine derivatization was demonstrated.

Authors+Show Affiliations

Chan KC
Analytical Chemistry Laboratory, SAIC Frederick, NCI-Frederick Cancer Research and Development Center, MD 21702, USA. chan@mail.ncifcrf.gov
Muschik GM
No affiliation info available
Issaq HJ
No affiliation info available

MeSH

Amino AcidsAngiotensin IICytochrome c GroupElectrophoresis, CapillaryFluorescamineFluorescent DyesPeptide FragmentsPeptidesProteinsSensitivity and SpecificitySpectrometry, FluorescenceTrypsin

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10879967

Citation

Chan, K C., et al. "Solid-state UV Laser-induced Fluorescence Detection in Capillary Electrophoresis." Electrophoresis, vol. 21, no. 10, 2000, pp. 2062-6.
Chan KC, Muschik GM, Issaq HJ. Solid-state UV laser-induced fluorescence detection in capillary electrophoresis. Electrophoresis. 2000;21(10):2062-6.
Chan, K. C., Muschik, G. M., & Issaq, H. J. (2000). Solid-state UV laser-induced fluorescence detection in capillary electrophoresis. Electrophoresis, 21(10), 2062-6.
Chan KC, Muschik GM, Issaq HJ. Solid-state UV Laser-induced Fluorescence Detection in Capillary Electrophoresis. Electrophoresis. 2000;21(10):2062-6. PubMed PMID: 10879967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solid-state UV laser-induced fluorescence detection in capillary electrophoresis. AU - Chan,K C, AU - Muschik,G M, AU - Issaq,H J, PY - 2000/7/6/pubmed PY - 2001/2/28/medline PY - 2000/7/6/entrez SP - 2062 EP - 6 JF - Electrophoresis JO - Electrophoresis VL - 21 IS - 10 N2 - Two solid-state UV lasers were applied to the laser-induced fluorescence (LIF) detection of various groups of compounds after separation by capillary electrophoresis. These lasers are thermoelectric-cooled, highly compact, and inexpensive. Such lasers provide few mW of quasi-continuous wave (CW) power which are sufficient and stable for LIF detection. Native fluorescence detection of tryptophan-containing proteins and peptides and related indoles was achieved at the nM level with the laser operating at 266 nm. Detection of fluorescamine-labeled amino acids and peptides was also possible at the nM level with the laser operating at 355 nm. Amino acids at a concentration as low as 10 ng/mL could be labeled with fluorescamine. Solid-state UV-LIF detection of the tryptic digest of cytochrome c after fluorescamine derivatization was demonstrated. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10879967/Solid_state_UV_laser_induced_fluorescence_detection_in_capillary_electrophoresis_ L2 - https://doi.org/10.1002/1522-2683(20000601)21:10<2062::AID-ELPS2062>3.0.CO;2-Z DB - PRIME DP - Unbound Medicine ER -