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Plasma total homocysteine and other thiols analyzed by capillary electrophoresis/laser-induced fluorescence detection: comparison with two other methods.
Electrophoresis. 2000 Jun; 21(10):2074-9.E

Abstract

We present a new analytical method for thiol quantification in plasma, based on the use of capillary electrophoresis (CE) and laser-induced fluorescence (LIF) to analyze 6-iodoacetamidofluorescein derivatives. Quantitative results of homocysteine, glutathione, cysteinylglycine, and cystationine are presented. A comparison of the quantitation of homocysteine in plasma, using high performance liquid chromatography/fluorescence detection and fluorescence polarization immunoassay is proposed. The results indicate that these techniques for plasma total homocysteine (tHcy) determination can be used interchangeably. The major advantage of CE-LIF is that it can quantitate the thiols in one run while keeping the price of consumables reasonable.

Authors+Show Affiliations

Caussé E
Laboratoire de Biochimie/INSERM U466, Centre Hospitalier Rangueil, Toulouse, France.
Malatray P
No affiliation info available
Calaf R
No affiliation info available
Charpiot P
No affiliation info available
Candito M
No affiliation info available
Bayle C
No affiliation info available
Valdiguié P
No affiliation info available
Salvayre R
No affiliation info available
Couderc F
No affiliation info available

MeSH

Chromatography, High Pressure LiquidElectrophoresis, CapillaryFluorescence PolarizationHomocysteineHumansImmunoassaySensitivity and SpecificitySpectrometry, FluorescenceSulfhydryl Compounds

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10879969

Citation

Caussé, E, et al. "Plasma Total Homocysteine and Other Thiols Analyzed By Capillary Electrophoresis/laser-induced Fluorescence Detection: Comparison With Two Other Methods." Electrophoresis, vol. 21, no. 10, 2000, pp. 2074-9.
Caussé E, Malatray P, Calaf R, et al. Plasma total homocysteine and other thiols analyzed by capillary electrophoresis/laser-induced fluorescence detection: comparison with two other methods. Electrophoresis. 2000;21(10):2074-9.
Caussé, E., Malatray, P., Calaf, R., Charpiot, P., Candito, M., Bayle, C., Valdiguié, P., Salvayre, R., & Couderc, F. (2000). Plasma total homocysteine and other thiols analyzed by capillary electrophoresis/laser-induced fluorescence detection: comparison with two other methods. Electrophoresis, 21(10), 2074-9.
Caussé E, et al. Plasma Total Homocysteine and Other Thiols Analyzed By Capillary Electrophoresis/laser-induced Fluorescence Detection: Comparison With Two Other Methods. Electrophoresis. 2000;21(10):2074-9. PubMed PMID: 10879969.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasma total homocysteine and other thiols analyzed by capillary electrophoresis/laser-induced fluorescence detection: comparison with two other methods. AU - Caussé,E, AU - Malatray,P, AU - Calaf,R, AU - Charpiot,P, AU - Candito,M, AU - Bayle,C, AU - Valdiguié,P, AU - Salvayre,R, AU - Couderc,F, PY - 2000/7/6/pubmed PY - 2001/2/28/medline PY - 2000/7/6/entrez SP - 2074 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 21 IS - 10 N2 - We present a new analytical method for thiol quantification in plasma, based on the use of capillary electrophoresis (CE) and laser-induced fluorescence (LIF) to analyze 6-iodoacetamidofluorescein derivatives. Quantitative results of homocysteine, glutathione, cysteinylglycine, and cystationine are presented. A comparison of the quantitation of homocysteine in plasma, using high performance liquid chromatography/fluorescence detection and fluorescence polarization immunoassay is proposed. The results indicate that these techniques for plasma total homocysteine (tHcy) determination can be used interchangeably. The major advantage of CE-LIF is that it can quantitate the thiols in one run while keeping the price of consumables reasonable. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10879969/Plasma_total_homocysteine_and_other_thiols_analyzed_by_capillary_electrophoresis/laser_induced_fluorescence_detection:_comparison_with_two_other_methods_ L2 - https://doi.org/10.1002/1522-2683(20000601)21:10<2074::AID-ELPS2074>3.0.CO;2-L DB - PRIME DP - Unbound Medicine ER -