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Alternative splicing of Xenopus alphafast-tropomyosin pre-mRNA during development: identification of determining sequences.
DNA Cell Biol. 2000 Jun; 19(6):365-76.DC

Abstract

The Xenopus alphafast-tropomyosin gene contains in its central part a set of mutually exclusive exons, designated 6A and 6B, which are incorporated into mRNA encoding, respectively, nonmuscle and muscle tropomyosins. In this study, we show that usage of both exons is strictly regulated during development, exon 6A being used in the oocyte and nonmuscle tissues of the embryo, while exon 6B is used in muscle tissues. An approach of transient embryo transgenesis was developed to study the mechanisms involved in the splice site choice during development. We demonstrate that a-tropomyosin minigenes driven by tissue-specific promoters that target gene expression in nonmuscle and muscle tissues recapitulate the splicing pattern of the endogenous gene. A mutational analysis showed that regulation occurred at both exons 6A and 6B in muscle and nonmuscle tissues. In this context, we have identified an element located in the intron downstream of 6A that participates in the recognition of the weak 5' splice site of exon 6A and the repression of exon 6B in nonmuscle cells.

Authors+Show Affiliations

UPR 41 CNRS, Université de Rennes I, Faculté de Médecine, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10882235

Citation

Duriez, P, et al. "Alternative Splicing of Xenopus Alphafast-tropomyosin pre-mRNA During Development: Identification of Determining Sequences." DNA and Cell Biology, vol. 19, no. 6, 2000, pp. 365-76.
Duriez P, Lesimple M, Allo MR, et al. Alternative splicing of Xenopus alphafast-tropomyosin pre-mRNA during development: identification of determining sequences. DNA Cell Biol. 2000;19(6):365-76.
Duriez, P., Lesimple, M., Allo, M. R., & Hardy, S. (2000). Alternative splicing of Xenopus alphafast-tropomyosin pre-mRNA during development: identification of determining sequences. DNA and Cell Biology, 19(6), 365-76.
Duriez P, et al. Alternative Splicing of Xenopus Alphafast-tropomyosin pre-mRNA During Development: Identification of Determining Sequences. DNA Cell Biol. 2000;19(6):365-76. PubMed PMID: 10882235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alternative splicing of Xenopus alphafast-tropomyosin pre-mRNA during development: identification of determining sequences. AU - Duriez,P, AU - Lesimple,M, AU - Allo,M R, AU - Hardy,S, PY - 2000/7/6/pubmed PY - 2000/7/6/medline PY - 2000/7/6/entrez SP - 365 EP - 76 JF - DNA and cell biology JO - DNA Cell Biol VL - 19 IS - 6 N2 - The Xenopus alphafast-tropomyosin gene contains in its central part a set of mutually exclusive exons, designated 6A and 6B, which are incorporated into mRNA encoding, respectively, nonmuscle and muscle tropomyosins. In this study, we show that usage of both exons is strictly regulated during development, exon 6A being used in the oocyte and nonmuscle tissues of the embryo, while exon 6B is used in muscle tissues. An approach of transient embryo transgenesis was developed to study the mechanisms involved in the splice site choice during development. We demonstrate that a-tropomyosin minigenes driven by tissue-specific promoters that target gene expression in nonmuscle and muscle tissues recapitulate the splicing pattern of the endogenous gene. A mutational analysis showed that regulation occurred at both exons 6A and 6B in muscle and nonmuscle tissues. In this context, we have identified an element located in the intron downstream of 6A that participates in the recognition of the weak 5' splice site of exon 6A and the repression of exon 6B in nonmuscle cells. SN - 1044-5498 UR - https://www.unboundmedicine.com/medline/citation/10882235/Alternative_splicing_of_Xenopus_alphafast_tropomyosin_pre_mRNA_during_development:_identification_of_determining_sequences_ L2 - https://www.liebertpub.com/doi/10.1089/10445490050043335?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -