Tags

Type your tag names separated by a space and hit enter

Long-term microglial and astroglial activation in the hippocampus of trimethyltin-intoxicated rat: stimulation of NGF and TrkA immunoreactivities in astroglia but not in microglia.

Abstract

In the present study we investigated the microglial and astroglial response after trimethyltin (TMT) exposure over a prolonged period of time. Male Wistar rats were given a single dose of TMT (8 mg/kg, i.p.) and survived 4, 7, 21, 60 and 180 days after the administration of the toxin. Histochemistry (Griffonia simplicifolia lectin staining) and immunocytochemistry for GFAP were applied to identify micro- and astroglial cells, respectively. To assess the trophic response of glial cells (NGF and TrkA expression), single or double staining experiments were performed. In addition, the biochemical evaluation of GFAP and NGF were carried out at chosen timepoints using immunoblotting technique and ELISA, respectively. The main findings of our study were as follows. (1) A protracted activation of microglia (at least up to 2 months posttreatment). (2) A long-lasting expression of GFAP immunoreactivity (at least up to 6 months posttreatment) and a steady increase in GFAP content (at least up to 2 months posttreatment). (3) The appearance of enormously enlarged, round-shape astrocytes exclusively localized to CA1 and observed 2 months posttreatment. (4) The stimulation of NGF and TrkA expression in reactive astrocytes. (5) The strongest activation of micro- and astroglia coincided with the most prominent neurodegeneration in the hippocampus, i.e., in CA4/CA3c and CA1. It is tempting to assume that the activation of glial cells in the hippocampal areas particularly vulnerable to TMT may affect neuronal fate after neurotoxic insult.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Neurophysiology, Nencki Institute of Experimental Biology, 3 Pasteur St, 02-093, Warsaw, Poland.

    Source

    MeSH

    Animals
    Astrocytes
    Cell Count
    Enzyme-Linked Immunosorbent Assay
    Glial Fibrillary Acidic Protein
    Gliosis
    Hippocampus
    Immunohistochemistry
    Male
    Microglia
    Nerve Growth Factor
    Neurons
    Rats
    Rats, Wistar
    Receptor, trkA
    Time
    Trimethyltin Compounds

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    10884604

    Citation

    Koczyk, D, and B Oderfeld-Nowak. "Long-term Microglial and Astroglial Activation in the Hippocampus of Trimethyltin-intoxicated Rat: Stimulation of NGF and TrkA Immunoreactivities in Astroglia but Not in Microglia." International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience, vol. 18, no. 6, 2000, pp. 591-606.
    Koczyk D, Oderfeld-Nowak B. Long-term microglial and astroglial activation in the hippocampus of trimethyltin-intoxicated rat: stimulation of NGF and TrkA immunoreactivities in astroglia but not in microglia. Int J Dev Neurosci. 2000;18(6):591-606.
    Koczyk, D., & Oderfeld-Nowak, B. (2000). Long-term microglial and astroglial activation in the hippocampus of trimethyltin-intoxicated rat: stimulation of NGF and TrkA immunoreactivities in astroglia but not in microglia. International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience, 18(6), pp. 591-606.
    Koczyk D, Oderfeld-Nowak B. Long-term Microglial and Astroglial Activation in the Hippocampus of Trimethyltin-intoxicated Rat: Stimulation of NGF and TrkA Immunoreactivities in Astroglia but Not in Microglia. Int J Dev Neurosci. 2000;18(6):591-606. PubMed PMID: 10884604.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Long-term microglial and astroglial activation in the hippocampus of trimethyltin-intoxicated rat: stimulation of NGF and TrkA immunoreactivities in astroglia but not in microglia. AU - Koczyk,D, AU - Oderfeld-Nowak,B, PY - 2000/7/8/pubmed PY - 2000/9/30/medline PY - 2000/7/8/entrez SP - 591 EP - 606 JF - International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience JO - Int. J. Dev. Neurosci. VL - 18 IS - 6 N2 - In the present study we investigated the microglial and astroglial response after trimethyltin (TMT) exposure over a prolonged period of time. Male Wistar rats were given a single dose of TMT (8 mg/kg, i.p.) and survived 4, 7, 21, 60 and 180 days after the administration of the toxin. Histochemistry (Griffonia simplicifolia lectin staining) and immunocytochemistry for GFAP were applied to identify micro- and astroglial cells, respectively. To assess the trophic response of glial cells (NGF and TrkA expression), single or double staining experiments were performed. In addition, the biochemical evaluation of GFAP and NGF were carried out at chosen timepoints using immunoblotting technique and ELISA, respectively. The main findings of our study were as follows. (1) A protracted activation of microglia (at least up to 2 months posttreatment). (2) A long-lasting expression of GFAP immunoreactivity (at least up to 6 months posttreatment) and a steady increase in GFAP content (at least up to 2 months posttreatment). (3) The appearance of enormously enlarged, round-shape astrocytes exclusively localized to CA1 and observed 2 months posttreatment. (4) The stimulation of NGF and TrkA expression in reactive astrocytes. (5) The strongest activation of micro- and astroglia coincided with the most prominent neurodegeneration in the hippocampus, i.e., in CA4/CA3c and CA1. It is tempting to assume that the activation of glial cells in the hippocampal areas particularly vulnerable to TMT may affect neuronal fate after neurotoxic insult. SN - 0736-5748 UR - https://www.unboundmedicine.com/medline/citation/10884604/Long_term_microglial_and_astroglial_activation_in_the_hippocampus_of_trimethyltin_intoxicated_rat:_stimulation_of_NGF_and_TrkA_immunoreactivities_in_astroglia_but_not_in_microglia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0736-5748(99)00111-2 DB - PRIME DP - Unbound Medicine ER -