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Auxin biosynthesis in maize kernels.
Plant Physiol. 2000 Jul; 123(3):1109-19.PP

Abstract

Auxin biosynthesis was analyzed in a maize (Zea mays) kernel culture system in which the seeds develop under physiological conditions similar to the in vivo situation. This system was modified for precursor feeding experiments. Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention of the 3, 3' bond. Conversion of Trp to IAA is not competed by indole. Labeling with the general precursors [U-(13)C(6)]glucose and [1, 2-(13)C(2)]acetate followed by retrobiosynthetic analysis strongly suggest that Trp-dependent IAA synthesis is the predominant route for auxin biosynthesis in the maize kernel. The synthesis of IAA from indole glycerol phosphate and IAA formation via condensation of indole with an acetyl-coenzyme A or phosphoenolpyruvate derived metabolite can be excluded.

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Authors+Show Affiliations

Glawischnig E
Lehrstuhl für Genetik, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching, Germany.
Tomas A
No affiliation info available
Eisenreich W
No affiliation info available
Spiteller P
No affiliation info available
Bacher A
No affiliation info available
Gierl A
No affiliation info available

MeSH

Amino AcidsGas Chromatography-Mass SpectrometryIndoleacetic AcidsMagnetic Resonance SpectroscopyPlant Growth RegulatorsSeedsTryptophanZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10889260

Citation

Glawischnig, E, et al. "Auxin Biosynthesis in Maize Kernels." Plant Physiology, vol. 123, no. 3, 2000, pp. 1109-19.
Glawischnig E, Tomas A, Eisenreich W, et al. Auxin biosynthesis in maize kernels. Plant Physiol. 2000;123(3):1109-19.
Glawischnig, E., Tomas, A., Eisenreich, W., Spiteller, P., Bacher, A., & Gierl, A. (2000). Auxin biosynthesis in maize kernels. Plant Physiology, 123(3), 1109-19.
Glawischnig E, et al. Auxin Biosynthesis in Maize Kernels. Plant Physiol. 2000;123(3):1109-19. PubMed PMID: 10889260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Auxin biosynthesis in maize kernels. AU - Glawischnig,E, AU - Tomas,A, AU - Eisenreich,W, AU - Spiteller,P, AU - Bacher,A, AU - Gierl,A, PY - 2000/7/13/pubmed PY - 2000/10/21/medline PY - 2000/7/13/entrez SP - 1109 EP - 19 JF - Plant physiology JO - Plant Physiol VL - 123 IS - 3 N2 - Auxin biosynthesis was analyzed in a maize (Zea mays) kernel culture system in which the seeds develop under physiological conditions similar to the in vivo situation. This system was modified for precursor feeding experiments. Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention of the 3, 3' bond. Conversion of Trp to IAA is not competed by indole. Labeling with the general precursors [U-(13)C(6)]glucose and [1, 2-(13)C(2)]acetate followed by retrobiosynthetic analysis strongly suggest that Trp-dependent IAA synthesis is the predominant route for auxin biosynthesis in the maize kernel. The synthesis of IAA from indole glycerol phosphate and IAA formation via condensation of indole with an acetyl-coenzyme A or phosphoenolpyruvate derived metabolite can be excluded. SN - 0032-0889 UR - https://www.unboundmedicine.com/medline/citation/10889260/Auxin_biosynthesis_in_maize_kernels_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=10889260 DB - PRIME DP - Unbound Medicine ER -