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Protective immunity against feline immunodeficiency virus induced by inoculation with vif-deleted proviral DNA.
Virology. 2000 Jul 20; 273(1):67-79.V

Abstract

To determine whether live-attenuated feline immunodeficiency virus (FIV) proviral DNA will induce protective immunity, a plasmid clone constructed with a FIV provirus containing a deletion in the viral accessory gene vif (FIV-pPPR-Deltavif) was inoculated as proviral DNA into four cats by the intramuscular route. After 43 weeks, these cats were boosted with the same proviral plasmid. Analysis of peripheral blood mononuclear cells at several time points after the primary and booster inoculations revealed no detectable virus or proviral DNA. At 6 weeks after the booster, immunized cats and additional naive control cats were challenged with a cell-free preparation of the infectious biological isolate FIV-PPR by the intraperitoneal route. Virus was detected after challenge in unvaccinated control cats but not in any of the FIV-pPPR-Deltavif-immunized cats. Both FIV Gag- and Env-specific cytotoxic T lymphocyte (CTL) activities were detected in peripheral blood cells of control cats after challenge infection, whereas only one of four cats immunized with FIV-pPPR-Deltavif DNA exhibited a measurable CTL response to Env following challenge. Although anti-Gag antibodies were not detected after both proviral DNA inoculation and challenge, anti-Env antibodies were found in FIV-pPPR-Deltavif-immunized cats after vaccination as well as after challenge. These findings indicate that inoculation with FIV-pPPR-Deltavif proviral DNA induced resistance to challenge with infectious FIV and that a vif deletion mutant may provide a relatively safe attenuated lentiviral vaccine.

Links

Publisher Full Text
linkinghub.elsevier.com
linkinghub.elsevier.com

Authors+Show Affiliations

Lockridge KM
Departments of Medicine and Epidemiology, University of California, Davis, California, 95616, USA.
Chien M
No affiliation info available
Dean GA
No affiliation info available
Stefano Cole K
No affiliation info available
Montelaro RC
No affiliation info available
Luciw PA
No affiliation info available
Sparger EE
No affiliation info available

MeSH

AnimalsAntibodies, ViralAntigens, ViralCatsDNA, ViralGene DeletionGene Products, envGene Products, gagGene Products, vifGenome, ViralImmunodeficiency Virus, FelineLentivirus InfectionsLymphocyte CountProvirusesT-Lymphocytes, CytotoxicTime FactorsVaccines, DNAViral Vaccines

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10891409

Citation

Lockridge, K M., et al. "Protective Immunity Against Feline Immunodeficiency Virus Induced By Inoculation With Vif-deleted Proviral DNA." Virology, vol. 273, no. 1, 2000, pp. 67-79.
Lockridge KM, Chien M, Dean GA, et al. Protective immunity against feline immunodeficiency virus induced by inoculation with vif-deleted proviral DNA. Virology. 2000;273(1):67-79.
Lockridge, K. M., Chien, M., Dean, G. A., Stefano Cole, K., Montelaro, R. C., Luciw, P. A., & Sparger, E. E. (2000). Protective immunity against feline immunodeficiency virus induced by inoculation with vif-deleted proviral DNA. Virology, 273(1), 67-79.
Lockridge KM, et al. Protective Immunity Against Feline Immunodeficiency Virus Induced By Inoculation With Vif-deleted Proviral DNA. Virology. 2000 Jul 20;273(1):67-79. PubMed PMID: 10891409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective immunity against feline immunodeficiency virus induced by inoculation with vif-deleted proviral DNA. AU - Lockridge,K M, AU - Chien,M, AU - Dean,G A, AU - Stefano Cole,K, AU - Montelaro,R C, AU - Luciw,P A, AU - Sparger,E E, PY - 2000/7/13/pubmed PY - 2000/8/19/medline PY - 2000/7/13/entrez SP - 67 EP - 79 JF - Virology JO - Virology VL - 273 IS - 1 N2 - To determine whether live-attenuated feline immunodeficiency virus (FIV) proviral DNA will induce protective immunity, a plasmid clone constructed with a FIV provirus containing a deletion in the viral accessory gene vif (FIV-pPPR-Deltavif) was inoculated as proviral DNA into four cats by the intramuscular route. After 43 weeks, these cats were boosted with the same proviral plasmid. Analysis of peripheral blood mononuclear cells at several time points after the primary and booster inoculations revealed no detectable virus or proviral DNA. At 6 weeks after the booster, immunized cats and additional naive control cats were challenged with a cell-free preparation of the infectious biological isolate FIV-PPR by the intraperitoneal route. Virus was detected after challenge in unvaccinated control cats but not in any of the FIV-pPPR-Deltavif-immunized cats. Both FIV Gag- and Env-specific cytotoxic T lymphocyte (CTL) activities were detected in peripheral blood cells of control cats after challenge infection, whereas only one of four cats immunized with FIV-pPPR-Deltavif DNA exhibited a measurable CTL response to Env following challenge. Although anti-Gag antibodies were not detected after both proviral DNA inoculation and challenge, anti-Env antibodies were found in FIV-pPPR-Deltavif-immunized cats after vaccination as well as after challenge. These findings indicate that inoculation with FIV-pPPR-Deltavif proviral DNA induced resistance to challenge with infectious FIV and that a vif deletion mutant may provide a relatively safe attenuated lentiviral vaccine. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/10891409/Protective_immunity_against_feline_immunodeficiency_virus_induced_by_inoculation_with_vif_deleted_proviral_DNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(00)90395-7 DB - PRIME DP - Unbound Medicine ER -