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Use of capillary electrophoresis and indirect detection to quantitate in-capillary enzyme-catalyzed microreactions.
Analyst. 2000 Apr; 125(4):685-8.A

Abstract

The use of capillary electrophoresis and indirect detection to quantify reaction products of in-capillary enzyme-catalyzed microreactions is described. Migrating in a capillary under conditions of electrophoresis, plugs of enzyme and substrate are injected and allowed to react. Capillary electrophoresis is subsequently used to measure the extent of reaction. This technique is demonstrated using two model systems: the conversion of fructose-1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate by fructose-biphosphate aldolase (ALD, EC 4.1.2.13), and the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate by fructose-1,6-bisphospatase (FBPase, EC 3.1.3.11). These procedures expand the use of the capillary as a microreactor and offer a new approach to analyzing enzyme-mediated reactions.

Authors+Show Affiliations

Zhang Y
Department of Chemistry and Biochemistry, California State University, Los Angeles 90032-8002, USA.
el-Maghrabi MR
No affiliation info available
Gomez FA
No affiliation info available

MeSH

Dihydroxyacetone PhosphateElectrophoresis, CapillaryFructose-BisphosphataseFructose-Bisphosphate AldolaseFructosediphosphatesFructosephosphatesGlyceraldehyde 3-Phosphate

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10892022

Citation

Zhang, Y, et al. "Use of Capillary Electrophoresis and Indirect Detection to Quantitate In-capillary Enzyme-catalyzed Microreactions." The Analyst, vol. 125, no. 4, 2000, pp. 685-8.
Zhang Y, el-Maghrabi MR, Gomez FA. Use of capillary electrophoresis and indirect detection to quantitate in-capillary enzyme-catalyzed microreactions. Analyst. 2000;125(4):685-8.
Zhang, Y., el-Maghrabi, M. R., & Gomez, F. A. (2000). Use of capillary electrophoresis and indirect detection to quantitate in-capillary enzyme-catalyzed microreactions. The Analyst, 125(4), 685-8.
Zhang Y, el-Maghrabi MR, Gomez FA. Use of Capillary Electrophoresis and Indirect Detection to Quantitate In-capillary Enzyme-catalyzed Microreactions. Analyst. 2000;125(4):685-8. PubMed PMID: 10892022.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of capillary electrophoresis and indirect detection to quantitate in-capillary enzyme-catalyzed microreactions. AU - Zhang,Y, AU - el-Maghrabi,M R, AU - Gomez,F A, PY - 2000/7/13/pubmed PY - 2000/8/6/medline PY - 2000/7/13/entrez SP - 685 EP - 8 JF - The Analyst JO - Analyst VL - 125 IS - 4 N2 - The use of capillary electrophoresis and indirect detection to quantify reaction products of in-capillary enzyme-catalyzed microreactions is described. Migrating in a capillary under conditions of electrophoresis, plugs of enzyme and substrate are injected and allowed to react. Capillary electrophoresis is subsequently used to measure the extent of reaction. This technique is demonstrated using two model systems: the conversion of fructose-1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate by fructose-biphosphate aldolase (ALD, EC 4.1.2.13), and the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate by fructose-1,6-bisphospatase (FBPase, EC 3.1.3.11). These procedures expand the use of the capillary as a microreactor and offer a new approach to analyzing enzyme-mediated reactions. SN - 0003-2654 UR - https://www.unboundmedicine.com/medline/citation/10892022/Use_of_capillary_electrophoresis_and_indirect_detection_to_quantitate_in_capillary_enzyme_catalyzed_microreactions_ L2 - https://doi.org/10.1039/a909092d DB - PRIME DP - Unbound Medicine ER -