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Slow muscle induction by Hedgehog signalling in vitro.
J Cell Sci. 2000 Aug; 113 (Pt 15):2695-703.JC

Abstract

Muscles are composed of several fibre types, the precise combination of which determines muscle function. Whereas neonatal and adult fibre type is influenced by a number of extrinsic factors, such as neural input and muscle load, there is little knowledge of how muscle cells are initially determined in the early embryo. In the zebrafish, fibres of the slow twitch class arise from precociously specified myoblasts that lie close to the midline whereas the remainder of the myotome differentiates as fast myosin expressing muscle. In vivo evidence has suggested the Sonic Hedgehog glycoprotein, secreted from the notochord, controls the formation of slow twitch and fast twitch muscle fates. Here we describe an in vitro culture system that we have developed to test directly the ability of zebrafish myoblasts to respond to exogenous Sonic Hedgehog peptide. We find that Sonic Hedgehog peptide can control the binary cell fate choice of embryonic zebrafish myoblasts in vitro. We have also used this culture system to assay the relative activities of different Hedgehog-family proteins and to investigate the possible involvement of heterotrimeric G-proteins in Hedgehog signal transduction.

Authors+Show Affiliations

Molecular Embryology Laboratory, Imperial Cancer Research Fund, London WC2A 3PX, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10893185

Citation

Norris, W, et al. "Slow Muscle Induction By Hedgehog Signalling in Vitro." Journal of Cell Science, vol. 113 (Pt 15), 2000, pp. 2695-703.
Norris W, Neyt C, Ingham PW, et al. Slow muscle induction by Hedgehog signalling in vitro. J Cell Sci. 2000;113 (Pt 15):2695-703.
Norris, W., Neyt, C., Ingham, P. W., & Currie, P. D. (2000). Slow muscle induction by Hedgehog signalling in vitro. Journal of Cell Science, 113 (Pt 15), 2695-703.
Norris W, et al. Slow Muscle Induction By Hedgehog Signalling in Vitro. J Cell Sci. 2000;113 (Pt 15):2695-703. PubMed PMID: 10893185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Slow muscle induction by Hedgehog signalling in vitro. AU - Norris,W, AU - Neyt,C, AU - Ingham,P W, AU - Currie,P D, PY - 2000/7/13/pubmed PY - 2000/9/23/medline PY - 2000/7/13/entrez SP - 2695 EP - 703 JF - Journal of cell science JO - J Cell Sci VL - 113 (Pt 15) N2 - Muscles are composed of several fibre types, the precise combination of which determines muscle function. Whereas neonatal and adult fibre type is influenced by a number of extrinsic factors, such as neural input and muscle load, there is little knowledge of how muscle cells are initially determined in the early embryo. In the zebrafish, fibres of the slow twitch class arise from precociously specified myoblasts that lie close to the midline whereas the remainder of the myotome differentiates as fast myosin expressing muscle. In vivo evidence has suggested the Sonic Hedgehog glycoprotein, secreted from the notochord, controls the formation of slow twitch and fast twitch muscle fates. Here we describe an in vitro culture system that we have developed to test directly the ability of zebrafish myoblasts to respond to exogenous Sonic Hedgehog peptide. We find that Sonic Hedgehog peptide can control the binary cell fate choice of embryonic zebrafish myoblasts in vitro. We have also used this culture system to assay the relative activities of different Hedgehog-family proteins and to investigate the possible involvement of heterotrimeric G-proteins in Hedgehog signal transduction. SN - 0021-9533 UR - https://www.unboundmedicine.com/medline/citation/10893185/Slow_muscle_induction_by_Hedgehog_signalling_in_vitro_ L2 - https://journals.biologists.com/jcs/article-lookup/doi/10.1242/jcs.113.15.2695 DB - PRIME DP - Unbound Medicine ER -