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HLA-E is expressed on trophoblast and interacts with CD94/NKG2 receptors on decidual NK cells.
Eur J Immunol. 2000 Jun; 30(6):1623-31.EJ

Abstract

Non-classical MHC class I molecule HLA-E is the ligand for CD94/NKG2 NK cell receptors. Surface expression of HLA-E requires binding of specific HLA class I leader sequences. The uterine mucosa in early pregnancy (decidua) is infiltrated by large numbers of NK cells, which are closely associated with placental trophoblast cells. In this study we demonstrate that trophoblast cells express HLA-E on their cell surface in addition to the previously reported expression of HLA-G and HLA-C. Furthermore, we show that the vast majority of decidual NK cells bind to HLA-E tetrameric complexes and this binding is inhibited by mAb to CD94. Thus, recognition of fetal HLA-E by decidual NK cells may play a key role in regulation of placentation. The functional consequences of decidual NK cell interaction were investigated in cytotoxicity assays using polyclonal decidual NK cells. The overall effect of CD94/NKG2 interaction with HLA-E is inhibition of cytotoxicity by decidual NK cells. However, since decidual NK cells are unable to kill trophoblast even in the presence of mAb to MHC class I molecules and NK cell receptors, HLA-E interaction with CD94/NKG2 receptors may regulate other functions besides cytolysis during implantation.

Authors+Show Affiliations

Department of Pathology, University of Cambridge. akk27@cam.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10898498

Citation

King, A, et al. "HLA-E Is Expressed On Trophoblast and Interacts With CD94/NKG2 Receptors On Decidual NK Cells." European Journal of Immunology, vol. 30, no. 6, 2000, pp. 1623-31.
King A, Allan DS, Bowen M, et al. HLA-E is expressed on trophoblast and interacts with CD94/NKG2 receptors on decidual NK cells. Eur J Immunol. 2000;30(6):1623-31.
King, A., Allan, D. S., Bowen, M., Powis, S. J., Joseph, S., Verma, S., Hiby, S. E., McMichael, A. J., Loke, Y. W., & Braud, V. M. (2000). HLA-E is expressed on trophoblast and interacts with CD94/NKG2 receptors on decidual NK cells. European Journal of Immunology, 30(6), 1623-31.
King A, et al. HLA-E Is Expressed On Trophoblast and Interacts With CD94/NKG2 Receptors On Decidual NK Cells. Eur J Immunol. 2000;30(6):1623-31. PubMed PMID: 10898498.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HLA-E is expressed on trophoblast and interacts with CD94/NKG2 receptors on decidual NK cells. AU - King,A, AU - Allan,D S, AU - Bowen,M, AU - Powis,S J, AU - Joseph,S, AU - Verma,S, AU - Hiby,S E, AU - McMichael,A J, AU - Loke,Y W, AU - Braud,V M, PY - 2000/7/18/pubmed PY - 2000/8/12/medline PY - 2000/7/18/entrez SP - 1623 EP - 31 JF - European journal of immunology JO - Eur. J. Immunol. VL - 30 IS - 6 N2 - Non-classical MHC class I molecule HLA-E is the ligand for CD94/NKG2 NK cell receptors. Surface expression of HLA-E requires binding of specific HLA class I leader sequences. The uterine mucosa in early pregnancy (decidua) is infiltrated by large numbers of NK cells, which are closely associated with placental trophoblast cells. In this study we demonstrate that trophoblast cells express HLA-E on their cell surface in addition to the previously reported expression of HLA-G and HLA-C. Furthermore, we show that the vast majority of decidual NK cells bind to HLA-E tetrameric complexes and this binding is inhibited by mAb to CD94. Thus, recognition of fetal HLA-E by decidual NK cells may play a key role in regulation of placentation. The functional consequences of decidual NK cell interaction were investigated in cytotoxicity assays using polyclonal decidual NK cells. The overall effect of CD94/NKG2 interaction with HLA-E is inhibition of cytotoxicity by decidual NK cells. However, since decidual NK cells are unable to kill trophoblast even in the presence of mAb to MHC class I molecules and NK cell receptors, HLA-E interaction with CD94/NKG2 receptors may regulate other functions besides cytolysis during implantation. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/10898498/HLA_E_is_expressed_on_trophoblast_and_interacts_with_CD94/NKG2_receptors_on_decidual_NK_cells_ L2 - https://doi.org/10.1002/1521-4141(200006)30:6<1623::AID-IMMU1623>3.0.CO;2-M DB - PRIME DP - Unbound Medicine ER -