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Molecular analyses of a repetitive DNA sequence in wheat (Triticum aestivum L.).
Genome. 2000 Jun; 43(3):556-63.G

Abstract

A repetitive sequence designated WE35 was isolated from wheat genomic DNA. This sequence consists of a 320-bp repeat unit and represents approximately 0.002% of the total wheat DNA. It is unidirectionally distributed either continuously or discretely in the genome. Ladder-like banding patterns were observed in Southern blots when the wheat genomic DNA was restricted with endonuclease enzymes EcoRI, HincII, NciI, and NdeI, which is characteristic for tandemly organized sequences. Two DNA fragments in p451 were frequently associated with the WE35 repetitive unit in a majority of lambda wheat genomic clones. A 475-bp fragment homologous to the 5'-end long terminal repeat (LTR) of cereal retroelements was also found in some lambda wheat genomic clones containing the repetitive unit. Physical mapping by fluorescence in situ hybridization (FISH) indicated that one pair of wheat chromosomes could be specifically detected with the WE35 positive probe p551. WE35 can be considered a chromosome-specific repetitive sequence. This repetitive unit could be used as a molecular marker for genetic, phylogenetic, and evolutionary studies in the tribe Triticeae.

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Authors+Show Affiliations

Ueng PP
Molecular Plant Pathology Lab, USDA-ARS, Beltsville, MD 20705, USA. pueng@asrr.arsusda.gov
Hang A
No affiliation info available
Tsang H
No affiliation info available
Vega JM
No affiliation info available
Wang L
No affiliation info available
Burton CS
No affiliation info available
He FT
No affiliation info available
Liu B
No affiliation info available

MeSH

Amino Acid SequenceBase SequenceBlotting, SouthernChromosome MappingDNA, PlantGenome, PlantIn Situ Hybridization, FluorescenceMolecular Sequence DataRepetitive Sequences, Nucleic AcidSequence AlignmentSequence Analysis, DNATriticum

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

10902721

Citation

Ueng, P P., et al. "Molecular Analyses of a Repetitive DNA Sequence in Wheat (Triticum Aestivum L.)." Genome, vol. 43, no. 3, 2000, pp. 556-63.
Ueng PP, Hang A, Tsang H, et al. Molecular analyses of a repetitive DNA sequence in wheat (Triticum aestivum L.). Genome. 2000;43(3):556-63.
Ueng, P. P., Hang, A., Tsang, H., Vega, J. M., Wang, L., Burton, C. S., He, F. T., & Liu, B. (2000). Molecular analyses of a repetitive DNA sequence in wheat (Triticum aestivum L.). Genome, 43(3), 556-63.
Ueng PP, et al. Molecular Analyses of a Repetitive DNA Sequence in Wheat (Triticum Aestivum L.). Genome. 2000;43(3):556-63. PubMed PMID: 10902721.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular analyses of a repetitive DNA sequence in wheat (Triticum aestivum L.). AU - Ueng,P P, AU - Hang,A, AU - Tsang,H, AU - Vega,J M, AU - Wang,L, AU - Burton,C S, AU - He,F T, AU - Liu,B, PY - 2000/7/21/pubmed PY - 2001/2/28/medline PY - 2000/7/21/entrez SP - 556 EP - 63 JF - Genome JO - Genome VL - 43 IS - 3 N2 - A repetitive sequence designated WE35 was isolated from wheat genomic DNA. This sequence consists of a 320-bp repeat unit and represents approximately 0.002% of the total wheat DNA. It is unidirectionally distributed either continuously or discretely in the genome. Ladder-like banding patterns were observed in Southern blots when the wheat genomic DNA was restricted with endonuclease enzymes EcoRI, HincII, NciI, and NdeI, which is characteristic for tandemly organized sequences. Two DNA fragments in p451 were frequently associated with the WE35 repetitive unit in a majority of lambda wheat genomic clones. A 475-bp fragment homologous to the 5'-end long terminal repeat (LTR) of cereal retroelements was also found in some lambda wheat genomic clones containing the repetitive unit. Physical mapping by fluorescence in situ hybridization (FISH) indicated that one pair of wheat chromosomes could be specifically detected with the WE35 positive probe p551. WE35 can be considered a chromosome-specific repetitive sequence. This repetitive unit could be used as a molecular marker for genetic, phylogenetic, and evolutionary studies in the tribe Triticeae. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/10902721/Molecular_analyses_of_a_repetitive_DNA_sequence_in_wheat__Triticum_aestivum_L___ L2 - https://cdnsciencepub.com/doi/10.1139/g99-143?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -