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Effect of cytokines on the proliferation/differentiation of stroma-initiating cells.
J Cell Physiol. 2000 Sep; 184(3):351-5.JC

Abstract

A culture system that identifies the precursor of murine bone marrow fibroblastic stromal cells (stroma-initiating cells, SIC) has been developed. In this system, mature fibroblasts are depleted by adherence to plastic dishes and the nonadherent cells are seeded at a low density, which results in the formation of colonies composed of fibroblastic cells. Macrophage colony-stimulating factor (M-CSF) has been shown to accelerate the colony formation in the system. In this study, we examined the stroma-inducing activity of a number of cytokines. Neither granulocyte-CSF, stem cell factor, interleukin (IL)-1, IL-6, transforming growth factor, epidermal growth factor, insulin-like growth factor, platelet-derived growth factor, nor fibroblast growth factor showed the activity. Similarly, tumor necrosis factor (TNF) did not show any stroma-inducing activity, but the factor inhibited the stromal colony formation induced by M-CSF. In this study, we found that granulocyte/macrophage-CSF (GM-CSF) and IL-3, as well as M-CSF had the stroma-inducing activity. Neither an additive nor synergistic effect was observed when the three factors were assayed in various combinations. The stroma-inducing activity of M-CSF, GM-CSF and IL-3 was observed even if lineage-negative bone marrow cells were used as target cells, suggesting that mature hematopoietic cells such as macrophages and granulocytes were not involved in the induction of stromal colony formation by these factors. Our results raise the possibility that GM-CSF and IL-3 as well as M-CSF stimulate the proliferation or differentiation of the precursor of bone marrow fibroblastic stromal cells.

Authors+Show Affiliations

Biochemical Research Laboratory, Morinaga Milk Industry Co. Ltd., Zama, Kanagawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10911366

Citation

Yamada, M, et al. "Effect of Cytokines On the Proliferation/differentiation of Stroma-initiating Cells." Journal of Cellular Physiology, vol. 184, no. 3, 2000, pp. 351-5.
Yamada M, Suzu S, Tanaka-Douzono M, et al. Effect of cytokines on the proliferation/differentiation of stroma-initiating cells. J Cell Physiol. 2000;184(3):351-5.
Yamada, M., Suzu, S., Tanaka-Douzono, M., Wakimoto, N., Hatake, K., Hayasawa, H., & Motoyoshi, K. (2000). Effect of cytokines on the proliferation/differentiation of stroma-initiating cells. Journal of Cellular Physiology, 184(3), 351-5.
Yamada M, et al. Effect of Cytokines On the Proliferation/differentiation of Stroma-initiating Cells. J Cell Physiol. 2000;184(3):351-5. PubMed PMID: 10911366.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of cytokines on the proliferation/differentiation of stroma-initiating cells. AU - Yamada,M, AU - Suzu,S, AU - Tanaka-Douzono,M, AU - Wakimoto,N, AU - Hatake,K, AU - Hayasawa,H, AU - Motoyoshi,K, PY - 2000/7/27/pubmed PY - 2000/9/2/medline PY - 2000/7/27/entrez SP - 351 EP - 5 JF - Journal of cellular physiology JO - J Cell Physiol VL - 184 IS - 3 N2 - A culture system that identifies the precursor of murine bone marrow fibroblastic stromal cells (stroma-initiating cells, SIC) has been developed. In this system, mature fibroblasts are depleted by adherence to plastic dishes and the nonadherent cells are seeded at a low density, which results in the formation of colonies composed of fibroblastic cells. Macrophage colony-stimulating factor (M-CSF) has been shown to accelerate the colony formation in the system. In this study, we examined the stroma-inducing activity of a number of cytokines. Neither granulocyte-CSF, stem cell factor, interleukin (IL)-1, IL-6, transforming growth factor, epidermal growth factor, insulin-like growth factor, platelet-derived growth factor, nor fibroblast growth factor showed the activity. Similarly, tumor necrosis factor (TNF) did not show any stroma-inducing activity, but the factor inhibited the stromal colony formation induced by M-CSF. In this study, we found that granulocyte/macrophage-CSF (GM-CSF) and IL-3, as well as M-CSF had the stroma-inducing activity. Neither an additive nor synergistic effect was observed when the three factors were assayed in various combinations. The stroma-inducing activity of M-CSF, GM-CSF and IL-3 was observed even if lineage-negative bone marrow cells were used as target cells, suggesting that mature hematopoietic cells such as macrophages and granulocytes were not involved in the induction of stromal colony formation by these factors. Our results raise the possibility that GM-CSF and IL-3 as well as M-CSF stimulate the proliferation or differentiation of the precursor of bone marrow fibroblastic stromal cells. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/10911366/Effect_of_cytokines_on_the_proliferation/differentiation_of_stroma_initiating_cells_ L2 - https://doi.org/10.1002/1097-4652(200009)184:3<351::AID-JCP9>3.0.CO;2-V DB - PRIME DP - Unbound Medicine ER -