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[Dynamics of anti-HEV ORF2, ORF3, IgM and IgG in serial sera of patients with hepatitis E and their clinical significance].
Zhonghua Yi Xue Za Zhi. 1998 Jul; 78(7):498-500.ZY

Abstract

OBJECTIVE

To study the dynamics of anti-HEV ORF2, ORF3, IgM and IgG in serial sera of patients with hepatitis E.

METHODS

Anti-HEV was detected in serial sera of 52 patients, by an enzyme-linked immunosorbent assay (EIA) based on two synthetic peptides separated or combined from open reading frame (ORF) 2 and 3 of HEV genome.

RESULTS

Both anti-ORF2 and anti-ORF3 in serum were at the highest levels in early acute phase of the disease, and declined gradually with the course, especially anti-ORF3. The sensitivity of the EIA using both ORF2 and ORF3 peptides was higher than that of either alone. The positive rates of anti-HEV IgM and anti-HEV IgG were separately 71.1% (32/45) and 97.8% (44/45) within 15 days after onset, then decreased more dramatically in IgM than in IgG with the course.

CONCLUSION

Both ORF2 and ORF3 proteins should be used for the development of anti-HEV EIA kit. It seems to be more specific in anti-HEV IgM and more sensitive in anti-HEV IgG as a diagnostic marker of hepatitis E.

Links

Publisher Full Text

Authors+Show Affiliations

Ruan B
Department of Infectious Diseases, First Hospital of Zhejiang Medical University, Hangzhou.
Zhuang H
No affiliation info available
Ma Y
No affiliation info available

MeSH

AdolescentAdultAgedEnzyme-Linked Immunosorbent AssayFemaleHepatitis AntibodiesHepatitis EHepatitis E virusHumansImmunoglobulin GImmunoglobulin MMaleMiddle AgedViral Proteins

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

10923456

Citation

Ruan, B, et al. "[Dynamics of anti-HEV ORF2, ORF3, IgM and IgG in Serial Sera of Patients With Hepatitis E and Their Clinical Significance]." Zhonghua Yi Xue Za Zhi, vol. 78, no. 7, 1998, pp. 498-500.
Ruan B, Zhuang H, Ma Y. [Dynamics of anti-HEV ORF2, ORF3, IgM and IgG in serial sera of patients with hepatitis E and their clinical significance]. Zhonghua Yi Xue Za Zhi. 1998;78(7):498-500.
Ruan, B., Zhuang, H., & Ma, Y. (1998). [Dynamics of anti-HEV ORF2, ORF3, IgM and IgG in serial sera of patients with hepatitis E and their clinical significance]. Zhonghua Yi Xue Za Zhi, 78(7), 498-500.
Ruan B, Zhuang H, Ma Y. [Dynamics of anti-HEV ORF2, ORF3, IgM and IgG in Serial Sera of Patients With Hepatitis E and Their Clinical Significance]. Zhonghua Yi Xue Za Zhi. 1998;78(7):498-500. PubMed PMID: 10923456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Dynamics of anti-HEV ORF2, ORF3, IgM and IgG in serial sera of patients with hepatitis E and their clinical significance]. AU - Ruan,B, AU - Zhuang,H, AU - Ma,Y, PY - 2000/8/3/pubmed PY - 2000/10/7/medline PY - 2000/8/3/entrez SP - 498 EP - 500 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 78 IS - 7 N2 - OBJECTIVE: To study the dynamics of anti-HEV ORF2, ORF3, IgM and IgG in serial sera of patients with hepatitis E. METHODS: Anti-HEV was detected in serial sera of 52 patients, by an enzyme-linked immunosorbent assay (EIA) based on two synthetic peptides separated or combined from open reading frame (ORF) 2 and 3 of HEV genome. RESULTS: Both anti-ORF2 and anti-ORF3 in serum were at the highest levels in early acute phase of the disease, and declined gradually with the course, especially anti-ORF3. The sensitivity of the EIA using both ORF2 and ORF3 peptides was higher than that of either alone. The positive rates of anti-HEV IgM and anti-HEV IgG were separately 71.1% (32/45) and 97.8% (44/45) within 15 days after onset, then decreased more dramatically in IgM than in IgG with the course. CONCLUSION: Both ORF2 and ORF3 proteins should be used for the development of anti-HEV EIA kit. It seems to be more specific in anti-HEV IgM and more sensitive in anti-HEV IgG as a diagnostic marker of hepatitis E. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/10923456/[Dynamics_of_anti_HEV_ORF2_ORF3_IgM_and_IgG_in_serial_sera_of_patients_with_hepatitis_E_and_their_clinical_significance]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0376-2491&year=1998&vol=78&issue=7&fpage=498 DB - PRIME DP - Unbound Medicine ER -