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Expression of major bone extracellular matrix proteins during embryonic osteogenesis in rat mandibles.
Anat Embryol (Berl) 2000; 202(1):31-7AE

Abstract

It is not known how bone proteins appear in the matrix before and after calcification during embryonic osteogenesis. The present study was designed to investigate expressions of the five major bone extracellular matrix proteins--i.e. type I collagen, osteonectin, osteopontin, bone sialoprotein and osteocalcin--during osteogenesis in rat embryonic mandibles immunohistochemically, and their involvement in calcification demonstrated by von Kossa staining. Wistar rat embryos 14 to 18 days post coitum were used. Osteogenesis was not seen in 14-day rat embryonic mandibles. Type I collagen was localized in the uncalcifed bone matrix in 15-day mandibles, where no other bone proteins showed immunoreactivity. Osteonectin, osteopontin, bone sialoprotein and osteocalcin appeared almost simultaneously in the calcified bone matrix of 16-day mandibles and accumulated continuously in 18-day mandibles. The present study suggested that type I collagen constitutes the basic framework of the bone matrix upon which the noncollagenous proteins are oriented to lead to calcification, whereas the noncollagenous proteins are deposited simultaneously by osteoblasts and are involved in calcification cooperatively.

Authors+Show Affiliations

Second Department of Oral Anatomy, Tohoku University School of Dentistry, Sendai, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10926093

Citation

Sasano, Y, et al. "Expression of Major Bone Extracellular Matrix Proteins During Embryonic Osteogenesis in Rat Mandibles." Anatomy and Embryology, vol. 202, no. 1, 2000, pp. 31-7.
Sasano Y, Zhu JX, Kamakura S, et al. Expression of major bone extracellular matrix proteins during embryonic osteogenesis in rat mandibles. Anat Embryol. 2000;202(1):31-7.
Sasano, Y., Zhu, J. X., Kamakura, S., Kusunoki, S., Mizoguchi, I., & Kagayama, M. (2000). Expression of major bone extracellular matrix proteins during embryonic osteogenesis in rat mandibles. Anatomy and Embryology, 202(1), pp. 31-7.
Sasano Y, et al. Expression of Major Bone Extracellular Matrix Proteins During Embryonic Osteogenesis in Rat Mandibles. Anat Embryol. 2000;202(1):31-7. PubMed PMID: 10926093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of major bone extracellular matrix proteins during embryonic osteogenesis in rat mandibles. AU - Sasano,Y, AU - Zhu,J X, AU - Kamakura,S, AU - Kusunoki,S, AU - Mizoguchi,I, AU - Kagayama,M, PY - 2000/8/5/pubmed PY - 2001/2/28/medline PY - 2000/8/5/entrez SP - 31 EP - 7 JF - Anatomy and embryology JO - Anat. Embryol. VL - 202 IS - 1 N2 - It is not known how bone proteins appear in the matrix before and after calcification during embryonic osteogenesis. The present study was designed to investigate expressions of the five major bone extracellular matrix proteins--i.e. type I collagen, osteonectin, osteopontin, bone sialoprotein and osteocalcin--during osteogenesis in rat embryonic mandibles immunohistochemically, and their involvement in calcification demonstrated by von Kossa staining. Wistar rat embryos 14 to 18 days post coitum were used. Osteogenesis was not seen in 14-day rat embryonic mandibles. Type I collagen was localized in the uncalcifed bone matrix in 15-day mandibles, where no other bone proteins showed immunoreactivity. Osteonectin, osteopontin, bone sialoprotein and osteocalcin appeared almost simultaneously in the calcified bone matrix of 16-day mandibles and accumulated continuously in 18-day mandibles. The present study suggested that type I collagen constitutes the basic framework of the bone matrix upon which the noncollagenous proteins are oriented to lead to calcification, whereas the noncollagenous proteins are deposited simultaneously by osteoblasts and are involved in calcification cooperatively. SN - 0340-2061 UR - https://www.unboundmedicine.com/medline/citation/10926093/Expression_of_major_bone_extracellular_matrix_proteins_during_embryonic_osteogenesis_in_rat_mandibles_ L2 - https://dx.doi.org/10.1007/pl00008242 DB - PRIME DP - Unbound Medicine ER -