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Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates.

Abstract

Seven isolates of Burkholderia pseudomallei from cases of melioidosis in human (2 isolates) and animal (2 isolates), cat (one isolate) and from soil samples (2 isolates) were examined for in vitro sensitivity to 14 antimicrobial agents and for presence of plasmid DNA. Randomly amplified polymorphic DNA (RAPD) analysis was used to type the isolates, using two arbitrary primers. All isolates were sensitive to chloramphenicol, kanamycin, carbenicillin, rifampicin, enrofloxacin, tetracycline and sulfamethoxazole-trimethoprim. No plasmid was detected in all the isolates tested. RADP fingerprinting demonstrated genomic relationship between isolates, which provides an effective method to study the epidemiology of the isolates examined.

Authors+Show Affiliations

Department of Biotechnology, Faculty of Food Science and Biotechnology, University Putra Malaysia, Serdang, Selangor, Malaysia. son@fsb.upm.edu.myNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10928372

Citation

Radu, S, et al. "Genotypic and Phenotypic Relationship in Burkholderia Pseudomallei Indicates Colonization With Closely Related Isolates." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 30, no. 4, 1999, pp. 760-3.
Radu S, Lihan S, Idris A, et al. Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates. Southeast Asian J Trop Med Public Health. 1999;30(4):760-3.
Radu, S., Lihan, S., Idris, A., Ling, O. W., Al-Haddawi, M. H., & Rusul, G. (1999). Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates. The Southeast Asian Journal of Tropical Medicine and Public Health, 30(4), 760-3.
Radu S, et al. Genotypic and Phenotypic Relationship in Burkholderia Pseudomallei Indicates Colonization With Closely Related Isolates. Southeast Asian J Trop Med Public Health. 1999;30(4):760-3. PubMed PMID: 10928372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates. AU - Radu,S, AU - Lihan,S, AU - Idris,A, AU - Ling,O W, AU - Al-Haddawi,M H, AU - Rusul,G, PY - 2000/8/6/pubmed PY - 2000/8/19/medline PY - 2000/8/6/entrez SP - 760 EP - 3 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 30 IS - 4 N2 - Seven isolates of Burkholderia pseudomallei from cases of melioidosis in human (2 isolates) and animal (2 isolates), cat (one isolate) and from soil samples (2 isolates) were examined for in vitro sensitivity to 14 antimicrobial agents and for presence of plasmid DNA. Randomly amplified polymorphic DNA (RAPD) analysis was used to type the isolates, using two arbitrary primers. All isolates were sensitive to chloramphenicol, kanamycin, carbenicillin, rifampicin, enrofloxacin, tetracycline and sulfamethoxazole-trimethoprim. No plasmid was detected in all the isolates tested. RADP fingerprinting demonstrated genomic relationship between isolates, which provides an effective method to study the epidemiology of the isolates examined. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/10928372/Genotypic_and_phenotypic_relationship_in_Burkholderia_pseudomallei_indicates_colonization_with_closely_related_isolates_ L2 - https://medlineplus.gov/antibiotics.html DB - PRIME DP - Unbound Medicine ER -