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Evidence that adenosine 5'-triphosphate is the third inhibitory non-adrenergic non-cholinergic neurotransmitter in the rat gastric fundus.
Br J Pharmacol. 2000 Aug; 130(7):1627-31.BJ

Abstract

In the rat gastric fundus, non-adrenergic, non-cholinergic (NANC) relaxations are mediated by nitric oxide (NO), vasoactive intestinal polypeptide (VIP), and a third, as yet unidentified, neurotransmitter. The possible involvement of adenosine 5'-triphosphate (ATP) in the NANC relaxations was examined using pyridoxalphosphate-6-azophenyl-2',5'-disulphonic acid (PPADS), apamin and desensitization to alpha,beta-methylene ATP. NANC responses were studied in the absence and presence of N(G)-nitro-L-arginine methyl ester (NAME; 100 microM) and alpha-chymotrypsin (1 u ml(-1)), to inhibit responses to NO and VIP, respectively. PPADS (100 microM), apamin (1 microM) and desensitization to alpha,beta-methylene ATP (10 microM, three additions) all significantly (P<0.05) reduced NANC relaxations to electrical field stimulation (0.5 - 4 Hz, 30 s trains) in longitudinal strips of rat gastric fundus and almost abolished the residual relaxation remaining in the presence of NAME and alpha-chymotrypsin. PPADS had no effect on responses to the NO-donor, sodium nitroprusside (SNP), or VIP. Apamin slightly reduced relaxations to SNP, but did not affect those to VIP, whereas desensitization to alpha,beta-methylene ATP markedly reduced responses to both SNP and VIP. The effects of PPADS and apamin in this study provide strong evidence that the third inhibitory NANC neurotransmitter in the rat gastric fundus is ATP.

Authors+Show Affiliations

Department of Anatomy and Cell Biology, University of Melbourne, Victoria 3010, Australia. K.Jenkinson@anatomy.unimelb.edu.auNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10928967

Citation

Jenkinson, K M., and J J. Reid. "Evidence That Adenosine 5'-triphosphate Is the Third Inhibitory Non-adrenergic Non-cholinergic Neurotransmitter in the Rat Gastric Fundus." British Journal of Pharmacology, vol. 130, no. 7, 2000, pp. 1627-31.
Jenkinson KM, Reid JJ. Evidence that adenosine 5'-triphosphate is the third inhibitory non-adrenergic non-cholinergic neurotransmitter in the rat gastric fundus. Br J Pharmacol. 2000;130(7):1627-31.
Jenkinson, K. M., & Reid, J. J. (2000). Evidence that adenosine 5'-triphosphate is the third inhibitory non-adrenergic non-cholinergic neurotransmitter in the rat gastric fundus. British Journal of Pharmacology, 130(7), 1627-31.
Jenkinson KM, Reid JJ. Evidence That Adenosine 5'-triphosphate Is the Third Inhibitory Non-adrenergic Non-cholinergic Neurotransmitter in the Rat Gastric Fundus. Br J Pharmacol. 2000;130(7):1627-31. PubMed PMID: 10928967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence that adenosine 5'-triphosphate is the third inhibitory non-adrenergic non-cholinergic neurotransmitter in the rat gastric fundus. AU - Jenkinson,K M, AU - Reid,J J, PY - 2000/8/6/pubmed PY - 2001/2/28/medline PY - 2000/8/6/entrez SP - 1627 EP - 31 JF - British journal of pharmacology JO - Br J Pharmacol VL - 130 IS - 7 N2 - In the rat gastric fundus, non-adrenergic, non-cholinergic (NANC) relaxations are mediated by nitric oxide (NO), vasoactive intestinal polypeptide (VIP), and a third, as yet unidentified, neurotransmitter. The possible involvement of adenosine 5'-triphosphate (ATP) in the NANC relaxations was examined using pyridoxalphosphate-6-azophenyl-2',5'-disulphonic acid (PPADS), apamin and desensitization to alpha,beta-methylene ATP. NANC responses were studied in the absence and presence of N(G)-nitro-L-arginine methyl ester (NAME; 100 microM) and alpha-chymotrypsin (1 u ml(-1)), to inhibit responses to NO and VIP, respectively. PPADS (100 microM), apamin (1 microM) and desensitization to alpha,beta-methylene ATP (10 microM, three additions) all significantly (P<0.05) reduced NANC relaxations to electrical field stimulation (0.5 - 4 Hz, 30 s trains) in longitudinal strips of rat gastric fundus and almost abolished the residual relaxation remaining in the presence of NAME and alpha-chymotrypsin. PPADS had no effect on responses to the NO-donor, sodium nitroprusside (SNP), or VIP. Apamin slightly reduced relaxations to SNP, but did not affect those to VIP, whereas desensitization to alpha,beta-methylene ATP markedly reduced responses to both SNP and VIP. The effects of PPADS and apamin in this study provide strong evidence that the third inhibitory NANC neurotransmitter in the rat gastric fundus is ATP. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/10928967/Evidence_that_adenosine_5'_triphosphate_is_the_third_inhibitory_non_adrenergic_non_cholinergic_neurotransmitter_in_the_rat_gastric_fundus_ L2 - https://doi.org/10.1038/sj.bjp.0703481 DB - PRIME DP - Unbound Medicine ER -