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The cis requirements for transcriptional activation by HilA, a virulence determinant encoded on SPI-1.
Mol Microbiol. 2000 Jul; 37(2):300-15.MM

Abstract

In several models of pathogenesis, Salmonella requires genes encoded on Salmonella pathogenicity island 1 (SPI-1) for virulence. In Salmonella enterica serovar Typhimurium (S. typhimurium), most SPI-1 genes are arranged in operons and are co-ordinately regulated in response to environmental signals via the SPI-1-encoded protein HilA. In order to understand how HilA controls the transcription of SPI-1 genes, we have analysed the invF and prgH promoters. We have reconstituted HilA-dependent activation of both promoters in Escherichia coli by supplying hilA on a plasmid, strongly suggesting that HilA acts directly on the promoters. By analysing the HilA-dependent activity of deletions and mutations in PinvF, we identified cis elements necessary for HilA-dependent activation. Through biochemical studies, we have defined a probable HilA-binding sequence in the invF promoter. This 'HilA box' is intact in the minimal promoter identified through deletion analysis, and it is disrupted in one class of PinvF mutants that has reduced activation by HilA. The prgH promoter also contains a HilA box in the same position relative to the +1 of transcription. This work is the first to connect HilA-dependent environmental regulation with a specific sequence in a SPI-1 virulence gene promoter.

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Authors+Show Affiliations

Lostroh CP
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
Bajaj V
No affiliation info available
Lee CA
No affiliation info available

MeSH

Bacterial ProteinsBase SequenceBlotting, WesternDNA-Binding ProteinsEscherichia coliGene Expression Regulation, BacterialMolecular Sequence DataMutagenesis, Site-DirectedPromoter Regions, GeneticSalmonella typhimuriumTrans-ActivatorsTranscription FactorsTranscriptional ActivationVirulence

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10931326

Citation

Lostroh, C P., et al. "The Cis Requirements for Transcriptional Activation By HilA, a Virulence Determinant Encoded On SPI-1." Molecular Microbiology, vol. 37, no. 2, 2000, pp. 300-15.
Lostroh CP, Bajaj V, Lee CA. The cis requirements for transcriptional activation by HilA, a virulence determinant encoded on SPI-1. Mol Microbiol. 2000;37(2):300-15.
Lostroh, C. P., Bajaj, V., & Lee, C. A. (2000). The cis requirements for transcriptional activation by HilA, a virulence determinant encoded on SPI-1. Molecular Microbiology, 37(2), 300-15.
Lostroh CP, Bajaj V, Lee CA. The Cis Requirements for Transcriptional Activation By HilA, a Virulence Determinant Encoded On SPI-1. Mol Microbiol. 2000;37(2):300-15. PubMed PMID: 10931326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The cis requirements for transcriptional activation by HilA, a virulence determinant encoded on SPI-1. AU - Lostroh,C P, AU - Bajaj,V, AU - Lee,C A, PY - 2000/8/10/pubmed PY - 2000/10/21/medline PY - 2000/8/10/entrez SP - 300 EP - 15 JF - Molecular microbiology JO - Mol Microbiol VL - 37 IS - 2 N2 - In several models of pathogenesis, Salmonella requires genes encoded on Salmonella pathogenicity island 1 (SPI-1) for virulence. In Salmonella enterica serovar Typhimurium (S. typhimurium), most SPI-1 genes are arranged in operons and are co-ordinately regulated in response to environmental signals via the SPI-1-encoded protein HilA. In order to understand how HilA controls the transcription of SPI-1 genes, we have analysed the invF and prgH promoters. We have reconstituted HilA-dependent activation of both promoters in Escherichia coli by supplying hilA on a plasmid, strongly suggesting that HilA acts directly on the promoters. By analysing the HilA-dependent activity of deletions and mutations in PinvF, we identified cis elements necessary for HilA-dependent activation. Through biochemical studies, we have defined a probable HilA-binding sequence in the invF promoter. This 'HilA box' is intact in the minimal promoter identified through deletion analysis, and it is disrupted in one class of PinvF mutants that has reduced activation by HilA. The prgH promoter also contains a HilA box in the same position relative to the +1 of transcription. This work is the first to connect HilA-dependent environmental regulation with a specific sequence in a SPI-1 virulence gene promoter. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/10931326/The_cis_requirements_for_transcriptional_activation_by_HilA_a_virulence_determinant_encoded_on_SPI_1_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=2000&volume=37&issue=2&spage=300 DB - PRIME DP - Unbound Medicine ER -