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Localization of natriuretic peptides and their activation of particulate guanylate cyclase and nitric oxide synthase in the retina.
J Comp Neurol. 2000 Sep 04; 424(4):689-700.JC

Abstract

In the vertebrate retina, cyclic guanosine monophosphate (cGMP) mediates photoreceptor signal transduction and modulates ion channel and gap junction conductivity. Although most previous studies have focused on its synthesis by nitric oxide (NO)-sensitive soluble guanylate cyclase, cGMP is also synthesized by NO-insensitive particulate guanylate cyclases (pGC). Natriuretic peptides and their associated pGC-coupled receptors have been reported in retina, but few studies have localized these natriuretic peptides or pGCs to specific retinal cells or demonstrated that activation of pGCs by natriuretic peptides increases cGMP synthesis. In this study, we immunocytochemically localized atrial, brain, and C-type natriuretic peptide-like immunoreactivity (ANP-LI, BNP-LI, and CNP-LI, respectively) in turtle retina by using isoform specific antisera, and determined the ability of each natriuretic peptide isoform to increase cGMP-like immunoreactivity (cGMP-LI) in retinal cells. ANP-LI and CNP-LI were localized in sparsely distributed amacrine cells with thin, intermittently varicose processes in the inner plexiform layer. BNP-LI was localized to abundant somata in the inner nuclear and ganglion cell layers and in specific amacrine and horizontal cells. Stimulation of turtle eyecups with each of these natriuretic peptides increased cGMP-LI in multistratified amacrine cells by means of NO-independent mechanisms in the central retina, and in select amacrine and bipolar cells in the peripheral retina by a nitric oxide-dependent mechanism. These results indicate that natriuretic peptides can modulate the synthesis of cGMP in select retinal neurons by two distinct signal transduction pathways in a regionally specific manner.

Authors+Show Affiliations

Department of Biology, Boston University, Boston, Massachusetts 02215, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10931490

Citation

Blute, T A., et al. "Localization of Natriuretic Peptides and Their Activation of Particulate Guanylate Cyclase and Nitric Oxide Synthase in the Retina." The Journal of Comparative Neurology, vol. 424, no. 4, 2000, pp. 689-700.
Blute TA, Lee HK, Huffmaster T, et al. Localization of natriuretic peptides and their activation of particulate guanylate cyclase and nitric oxide synthase in the retina. J Comp Neurol. 2000;424(4):689-700.
Blute, T. A., Lee, H. K., Huffmaster, T., Haverkamp, S., & Eldred, W. D. (2000). Localization of natriuretic peptides and their activation of particulate guanylate cyclase and nitric oxide synthase in the retina. The Journal of Comparative Neurology, 424(4), 689-700.
Blute TA, et al. Localization of Natriuretic Peptides and Their Activation of Particulate Guanylate Cyclase and Nitric Oxide Synthase in the Retina. J Comp Neurol. 2000 Sep 4;424(4):689-700. PubMed PMID: 10931490.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Localization of natriuretic peptides and their activation of particulate guanylate cyclase and nitric oxide synthase in the retina. AU - Blute,T A, AU - Lee,H K, AU - Huffmaster,T, AU - Haverkamp,S, AU - Eldred,W D, PY - 2000/8/10/pubmed PY - 2000/9/9/medline PY - 2000/8/10/entrez SP - 689 EP - 700 JF - The Journal of comparative neurology JO - J Comp Neurol VL - 424 IS - 4 N2 - In the vertebrate retina, cyclic guanosine monophosphate (cGMP) mediates photoreceptor signal transduction and modulates ion channel and gap junction conductivity. Although most previous studies have focused on its synthesis by nitric oxide (NO)-sensitive soluble guanylate cyclase, cGMP is also synthesized by NO-insensitive particulate guanylate cyclases (pGC). Natriuretic peptides and their associated pGC-coupled receptors have been reported in retina, but few studies have localized these natriuretic peptides or pGCs to specific retinal cells or demonstrated that activation of pGCs by natriuretic peptides increases cGMP synthesis. In this study, we immunocytochemically localized atrial, brain, and C-type natriuretic peptide-like immunoreactivity (ANP-LI, BNP-LI, and CNP-LI, respectively) in turtle retina by using isoform specific antisera, and determined the ability of each natriuretic peptide isoform to increase cGMP-like immunoreactivity (cGMP-LI) in retinal cells. ANP-LI and CNP-LI were localized in sparsely distributed amacrine cells with thin, intermittently varicose processes in the inner plexiform layer. BNP-LI was localized to abundant somata in the inner nuclear and ganglion cell layers and in specific amacrine and horizontal cells. Stimulation of turtle eyecups with each of these natriuretic peptides increased cGMP-LI in multistratified amacrine cells by means of NO-independent mechanisms in the central retina, and in select amacrine and bipolar cells in the peripheral retina by a nitric oxide-dependent mechanism. These results indicate that natriuretic peptides can modulate the synthesis of cGMP in select retinal neurons by two distinct signal transduction pathways in a regionally specific manner. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/10931490/Localization_of_natriuretic_peptides_and_their_activation_of_particulate_guanylate_cyclase_and_nitric_oxide_synthase_in_the_retina_ DB - PRIME DP - Unbound Medicine ER -