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GABA- and glycine-immunoreactive terminals contacting motoneurons in lamprey spinal cord.
J Chem Neuroanat. 2000 Jun; 19(2):69-80.JC

Abstract

Double postembedding GABA- and glycine-immunostaining was performed on the lamprey (Lampetra fluviatilis) spinal cord after previous HRP labeling of motoneurons. Immunopositive boutons contacting motoneurons were counted and distinguished as GABA (39%), glycine (30%) and both GABA+glycine-immunopositive (31%). Densely-packed, flattened synaptic vesicles were only observed in glycine-immunopositive boutons while GABA-immunoreactive and GABA+glycine-immunoreactive boutons contained rounded or oval synaptic vesicles. Dense-core vesicles of different diameters were associated with conventional synaptic vesicles in 74% of GABA-only-immunopositive boutons, 50% of double GABA+glycine-immunopositive boutons, but were only observed in 9% of glycine-only-immunopositive boutons. The presence of terminals immunoreactive to either GABA or glycine contacting the motoneurons suggests that there is a morphological substrate for both GABAergic and glycinergic postsynaptic inhibition of motoneurons in the lamprey spinal cord.

Authors+Show Affiliations

Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10936743

Citation

Vesselkin, N P., et al. "GABA- and Glycine-immunoreactive Terminals Contacting Motoneurons in Lamprey Spinal Cord." Journal of Chemical Neuroanatomy, vol. 19, no. 2, 2000, pp. 69-80.
Vesselkin NP, Rio JP, Adanina VO, et al. GABA- and glycine-immunoreactive terminals contacting motoneurons in lamprey spinal cord. J Chem Neuroanat. 2000;19(2):69-80.
Vesselkin, N. P., Rio, J. P., Adanina, V. O., & Repérant, J. (2000). GABA- and glycine-immunoreactive terminals contacting motoneurons in lamprey spinal cord. Journal of Chemical Neuroanatomy, 19(2), 69-80.
Vesselkin NP, et al. GABA- and Glycine-immunoreactive Terminals Contacting Motoneurons in Lamprey Spinal Cord. J Chem Neuroanat. 2000;19(2):69-80. PubMed PMID: 10936743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GABA- and glycine-immunoreactive terminals contacting motoneurons in lamprey spinal cord. AU - Vesselkin,N P, AU - Rio,J P, AU - Adanina,V O, AU - Repérant,J, PY - 2000/8/11/pubmed PY - 2000/9/30/medline PY - 2000/8/11/entrez SP - 69 EP - 80 JF - Journal of chemical neuroanatomy JO - J. Chem. Neuroanat. VL - 19 IS - 2 N2 - Double postembedding GABA- and glycine-immunostaining was performed on the lamprey (Lampetra fluviatilis) spinal cord after previous HRP labeling of motoneurons. Immunopositive boutons contacting motoneurons were counted and distinguished as GABA (39%), glycine (30%) and both GABA+glycine-immunopositive (31%). Densely-packed, flattened synaptic vesicles were only observed in glycine-immunopositive boutons while GABA-immunoreactive and GABA+glycine-immunoreactive boutons contained rounded or oval synaptic vesicles. Dense-core vesicles of different diameters were associated with conventional synaptic vesicles in 74% of GABA-only-immunopositive boutons, 50% of double GABA+glycine-immunopositive boutons, but were only observed in 9% of glycine-only-immunopositive boutons. The presence of terminals immunoreactive to either GABA or glycine contacting the motoneurons suggests that there is a morphological substrate for both GABAergic and glycinergic postsynaptic inhibition of motoneurons in the lamprey spinal cord. SN - 0891-0618 UR - https://www.unboundmedicine.com/medline/citation/10936743/GABA__and_glycine_immunoreactive_terminals_contacting_motoneurons_in_lamprey_spinal_cord_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-0618(00)00054-5 DB - PRIME DP - Unbound Medicine ER -