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Decreased colonic mucus in rats with loperamide-induced constipation.
Comp Biochem Physiol A Mol Integr Physiol. 2000 Jun; 126(2):203-12.CB

Abstract

Constipation is a risk factor of colorectal cancer. Mucin is a major component of lumenal mucus, which protects the colorectal mucosa against mechanical and chemical damage. The aim of this study was to evaluate mucus production and to quantitate lumen mucus in a rat model of spastic constipation. We induced constipation with loperamide (1.5 mg/kg), and histochemically evaluated mucus production and the thickness of the mucus layer at the fecal surface. We quantitated the mucus attached to the mucosal surface using colonic perfusion with N-acetylcysteine. While more feces remained in the colon, there was less fecal excretion and lower fecal water content in loperamide-administered rats than in control rats. Crypt epithelial cells contained less mucus in constipated rats than in control rats. The mucus layer at the fecal surface was thinner and less mucus was recovered from the mucosal surface in constipated rats than in control rats. Mucus production of crypt epithelial cells and mucus at the fecal and mucosal surface were reduced by loperamide-induced constipation.

Authors+Show Affiliations

Biological Science Laboratories, Kao Corporation, 2606 Akabane, 321-3497 Ichikai-machi, Haga-gun, Tochigi, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10936760

Citation

Shimotoyodome, A, et al. "Decreased Colonic Mucus in Rats With Loperamide-induced Constipation." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 126, no. 2, 2000, pp. 203-12.
Shimotoyodome A, Meguro S, Hase T, et al. Decreased colonic mucus in rats with loperamide-induced constipation. Comp Biochem Physiol A Mol Integr Physiol. 2000;126(2):203-12.
Shimotoyodome, A., Meguro, S., Hase, T., Tokimitsu, I., & Sakata, T. (2000). Decreased colonic mucus in rats with loperamide-induced constipation. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 126(2), 203-12.
Shimotoyodome A, et al. Decreased Colonic Mucus in Rats With Loperamide-induced Constipation. Comp Biochem Physiol A Mol Integr Physiol. 2000;126(2):203-12. PubMed PMID: 10936760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Decreased colonic mucus in rats with loperamide-induced constipation. AU - Shimotoyodome,A, AU - Meguro,S, AU - Hase,T, AU - Tokimitsu,I, AU - Sakata,T, PY - 2000/8/11/pubmed PY - 2000/10/7/medline PY - 2000/8/11/entrez SP - 203 EP - 12 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 126 IS - 2 N2 - Constipation is a risk factor of colorectal cancer. Mucin is a major component of lumenal mucus, which protects the colorectal mucosa against mechanical and chemical damage. The aim of this study was to evaluate mucus production and to quantitate lumen mucus in a rat model of spastic constipation. We induced constipation with loperamide (1.5 mg/kg), and histochemically evaluated mucus production and the thickness of the mucus layer at the fecal surface. We quantitated the mucus attached to the mucosal surface using colonic perfusion with N-acetylcysteine. While more feces remained in the colon, there was less fecal excretion and lower fecal water content in loperamide-administered rats than in control rats. Crypt epithelial cells contained less mucus in constipated rats than in control rats. The mucus layer at the fecal surface was thinner and less mucus was recovered from the mucosal surface in constipated rats than in control rats. Mucus production of crypt epithelial cells and mucus at the fecal and mucosal surface were reduced by loperamide-induced constipation. SN - 1095-6433 UR - https://www.unboundmedicine.com/medline/citation/10936760/Decreased_colonic_mucus_in_rats_with_loperamide_induced_constipation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(00)00194-X DB - PRIME DP - Unbound Medicine ER -