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Construction of plasmid vectors bearing a NotI-expression cassette based on the lac promoter.
Int Microbiol. 1999 Mar; 2(1):29-31.IM

Abstract

We have constructed two plasmid vectors for cloning and expression of DNA fragments controlled by the lac promoter as a NotI-expression cassette. Whereas plasmid pSJ33 allows mobilization of the expression cassette into a wide variety of Gram-negative bacteria by RP4-mediated conjugation, the low-copy-number plasmid pSJP18Not facilitates cloning and expression in Escherichia coli when high gene dosage may be detrimental. In addition to their suitable cloning features (e.g. multiple cloning site, lacZ alpha fragment, compatible with ColE1-derived vectors), these plasmids are particularly useful as auxiliary vectors for cloning of the expression cassettes at the NotI site of mini-transposon elements [1, 2] and their eventual stable insertion into the host chromosome.

Authors+Show Affiliations

Division of Microbiology, GBF-National Research Centre for Biotechnology, Braunschweig, Germany.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10943388

Citation

Jaenecke, S, and E Díaz. "Construction of Plasmid Vectors Bearing a NotI-expression Cassette Based On the Lac Promoter." International Microbiology : the Official Journal of the Spanish Society for Microbiology, vol. 2, no. 1, 1999, pp. 29-31.
Jaenecke S, Díaz E. Construction of plasmid vectors bearing a NotI-expression cassette based on the lac promoter. Int Microbiol. 1999;2(1):29-31.
Jaenecke, S., & Díaz, E. (1999). Construction of plasmid vectors bearing a NotI-expression cassette based on the lac promoter. International Microbiology : the Official Journal of the Spanish Society for Microbiology, 2(1), 29-31.
Jaenecke S, Díaz E. Construction of Plasmid Vectors Bearing a NotI-expression Cassette Based On the Lac Promoter. Int Microbiol. 1999;2(1):29-31. PubMed PMID: 10943388.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of plasmid vectors bearing a NotI-expression cassette based on the lac promoter. AU - Jaenecke,S, AU - Díaz,E, PY - 2000/12/8/pubmed PY - 2000/12/8/medline PY - 2000/12/8/entrez SP - 29 EP - 31 JF - International microbiology : the official journal of the Spanish Society for Microbiology JO - Int. Microbiol. VL - 2 IS - 1 N2 - We have constructed two plasmid vectors for cloning and expression of DNA fragments controlled by the lac promoter as a NotI-expression cassette. Whereas plasmid pSJ33 allows mobilization of the expression cassette into a wide variety of Gram-negative bacteria by RP4-mediated conjugation, the low-copy-number plasmid pSJP18Not facilitates cloning and expression in Escherichia coli when high gene dosage may be detrimental. In addition to their suitable cloning features (e.g. multiple cloning site, lacZ alpha fragment, compatible with ColE1-derived vectors), these plasmids are particularly useful as auxiliary vectors for cloning of the expression cassettes at the NotI site of mini-transposon elements [1, 2] and their eventual stable insertion into the host chromosome. SN - 1139-6709 UR - https://www.unboundmedicine.com/medline/citation/10943388/Construction_of_plasmid_vectors_bearing_a_NotI_expression_cassette_based_on_the_lac_promoter_ L2 - http://hdl.handle.net/10261/101581 DB - PRIME DP - Unbound Medicine ER -