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Expression of porcine transcription factors and genes related to fatty acid metabolism in different tissues and genetic populations.
J Anim Sci. 2000 Aug; 78(8):2127-34.JA

Abstract

Several transcription factors are involved in regulating lipid metabolism in various tissues of animals. Adipocyte determination and differentiation-dependent factor 1 (ADD1), peroxisome proliferator activated receptor alpha (PPAR alpha), and peroxisome proliferator activated receptor gamma (PPAR gamma) regulate both lipogenesis and fatty acid oxidation. We determined the tissue distribution and genetic difference in mRNA concentrations of these transcription factors in two genetic populations of pigs (Newsham XL-sired Newsham Landrace x Large White Duroc and Duroc-sired US Yorkshire x Duroc-Landrace). We also determined the tissue distribution and genetic difference in the mRNA concentration of fatty acid synthase (FAS) and acyl-CoA oxidase (ACO). Our data showed that ADD1 was highly expressed in adipose tissue and liver and that mRNA concentrations of ADD1 were similar between the two genotypes. The PPAR alpha mRNA concentration was high in adipose tissue and was similar between the two genotypes. In both populations, PPAR gamma mRNA was detected only in adipose tissue. There was no difference between the two genotypes in PPAR gamma mRNA concentration. The ACO mRNA was expressed in adipose tissue, skeletal muscle, and liver with no difference between genotypes. The FAS mRNA concentration in adipose tissue was seven times higher than that in the liver. There was no detectable FAS mRNA in skeletal muscle. These data support the concept that pig adipose tissue has considerable capability for fatty acid oxidation and synthesis. The uniqueness of expression patterns for FAS and ADD1 mRNA further indicates that adipose tissue is significantly involved in fatty acid and triacylglycerol synthesis in pigs.

Authors+Show Affiliations

USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030-2600, USA. sding@bcm.tmc.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

10947099

Citation

Ding, S T., et al. "Expression of Porcine Transcription Factors and Genes Related to Fatty Acid Metabolism in Different Tissues and Genetic Populations." Journal of Animal Science, vol. 78, no. 8, 2000, pp. 2127-34.
Ding ST, Schinckel AP, Weber TE, et al. Expression of porcine transcription factors and genes related to fatty acid metabolism in different tissues and genetic populations. J Anim Sci. 2000;78(8):2127-34.
Ding, S. T., Schinckel, A. P., Weber, T. E., & Mersmann, H. J. (2000). Expression of porcine transcription factors and genes related to fatty acid metabolism in different tissues and genetic populations. Journal of Animal Science, 78(8), 2127-34.
Ding ST, et al. Expression of Porcine Transcription Factors and Genes Related to Fatty Acid Metabolism in Different Tissues and Genetic Populations. J Anim Sci. 2000;78(8):2127-34. PubMed PMID: 10947099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of porcine transcription factors and genes related to fatty acid metabolism in different tissues and genetic populations. AU - Ding,S T, AU - Schinckel,A P, AU - Weber,T E, AU - Mersmann,H J, PY - 2000/8/18/pubmed PY - 2000/9/19/medline PY - 2000/8/18/entrez SP - 2127 EP - 34 JF - Journal of animal science JO - J Anim Sci VL - 78 IS - 8 N2 - Several transcription factors are involved in regulating lipid metabolism in various tissues of animals. Adipocyte determination and differentiation-dependent factor 1 (ADD1), peroxisome proliferator activated receptor alpha (PPAR alpha), and peroxisome proliferator activated receptor gamma (PPAR gamma) regulate both lipogenesis and fatty acid oxidation. We determined the tissue distribution and genetic difference in mRNA concentrations of these transcription factors in two genetic populations of pigs (Newsham XL-sired Newsham Landrace x Large White Duroc and Duroc-sired US Yorkshire x Duroc-Landrace). We also determined the tissue distribution and genetic difference in the mRNA concentration of fatty acid synthase (FAS) and acyl-CoA oxidase (ACO). Our data showed that ADD1 was highly expressed in adipose tissue and liver and that mRNA concentrations of ADD1 were similar between the two genotypes. The PPAR alpha mRNA concentration was high in adipose tissue and was similar between the two genotypes. In both populations, PPAR gamma mRNA was detected only in adipose tissue. There was no difference between the two genotypes in PPAR gamma mRNA concentration. The ACO mRNA was expressed in adipose tissue, skeletal muscle, and liver with no difference between genotypes. The FAS mRNA concentration in adipose tissue was seven times higher than that in the liver. There was no detectable FAS mRNA in skeletal muscle. These data support the concept that pig adipose tissue has considerable capability for fatty acid oxidation and synthesis. The uniqueness of expression patterns for FAS and ADD1 mRNA further indicates that adipose tissue is significantly involved in fatty acid and triacylglycerol synthesis in pigs. SN - 0021-8812 UR - https://www.unboundmedicine.com/medline/citation/10947099/Expression_of_porcine_transcription_factors_and_genes_related_to_fatty_acid_metabolism_in_different_tissues_and_genetic_populations_ DB - PRIME DP - Unbound Medicine ER -