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Human bronchoalveolar lavage fluid protein two-dimensional database: study of interstitial lung diseases.
Electrophoresis. 2000 Jul; 21(13):2703-12.E

Abstract

Recently, we published an analytical two-dimensional electrophoresis (2-DE) protein map of human bronchoalveolar lavage fluid (BALF) using a pool of BALFs from various patients. In this report, the effect of lung disorders on the protein composition of the lung epithelial lining fluid was investigated by 2-DE of BALFs from individual patients with well-defined interstitial lung diseases: sarcoidosis, idiopathic pulmonary fibrosis (IPF) and hypersensitivity pneumonitis (HP), using improved experimental conditions. On these gels, about 600-1000 stained protein spots could be identified in a BALF sample containing 25 microg of protein, and our original human BALF protein database has, therefore, been considerably extended. Altogether, 429 protein spots corresponding to 66 different proteins (including isoforms, protein subunits and fragments) were identified by microsequence analysis and by matching with a human blood plasma 2-DE protein map available in the SWISS-2DPAGE database. A human 2-DE BALF database was established and is available on the World Wide Web (http://www.umh.ac.be/-biochim/proteomic.htm+ ++). The significance of the modifications observed between the different lung pathologies is discussed with the aim of understanding the mechanistic bases of lung disease pathogenesis and finding new potential lung markers of disorders.

Authors+Show Affiliations

Department of Biological Chemistry, University of Mons-Hainaut, Belgium. rwattiez@ben.vub.ac.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10949149

Citation

Wattiez, R, et al. "Human Bronchoalveolar Lavage Fluid Protein Two-dimensional Database: Study of Interstitial Lung Diseases." Electrophoresis, vol. 21, no. 13, 2000, pp. 2703-12.
Wattiez R, Hermans C, Cruyt C, et al. Human bronchoalveolar lavage fluid protein two-dimensional database: study of interstitial lung diseases. Electrophoresis. 2000;21(13):2703-12.
Wattiez, R., Hermans, C., Cruyt, C., Bernard, A., & Falmagne, P. (2000). Human bronchoalveolar lavage fluid protein two-dimensional database: study of interstitial lung diseases. Electrophoresis, 21(13), 2703-12.
Wattiez R, et al. Human Bronchoalveolar Lavage Fluid Protein Two-dimensional Database: Study of Interstitial Lung Diseases. Electrophoresis. 2000;21(13):2703-12. PubMed PMID: 10949149.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human bronchoalveolar lavage fluid protein two-dimensional database: study of interstitial lung diseases. AU - Wattiez,R, AU - Hermans,C, AU - Cruyt,C, AU - Bernard,A, AU - Falmagne,P, PY - 2000/8/19/pubmed PY - 2001/2/28/medline PY - 2000/8/19/entrez SP - 2703 EP - 12 JF - Electrophoresis JO - Electrophoresis VL - 21 IS - 13 N2 - Recently, we published an analytical two-dimensional electrophoresis (2-DE) protein map of human bronchoalveolar lavage fluid (BALF) using a pool of BALFs from various patients. In this report, the effect of lung disorders on the protein composition of the lung epithelial lining fluid was investigated by 2-DE of BALFs from individual patients with well-defined interstitial lung diseases: sarcoidosis, idiopathic pulmonary fibrosis (IPF) and hypersensitivity pneumonitis (HP), using improved experimental conditions. On these gels, about 600-1000 stained protein spots could be identified in a BALF sample containing 25 microg of protein, and our original human BALF protein database has, therefore, been considerably extended. Altogether, 429 protein spots corresponding to 66 different proteins (including isoforms, protein subunits and fragments) were identified by microsequence analysis and by matching with a human blood plasma 2-DE protein map available in the SWISS-2DPAGE database. A human 2-DE BALF database was established and is available on the World Wide Web (http://www.umh.ac.be/-biochim/proteomic.htm+ ++). The significance of the modifications observed between the different lung pathologies is discussed with the aim of understanding the mechanistic bases of lung disease pathogenesis and finding new potential lung markers of disorders. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10949149/Human_bronchoalveolar_lavage_fluid_protein_two_dimensional_database:_study_of_interstitial_lung_diseases_ L2 - https://doi.org/10.1002/1522-2683(20000701)21:13<2703::AID-ELPS2703>3.0.CO;2-W DB - PRIME DP - Unbound Medicine ER -