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Direct action of the nodal-related signal cyclops in induction of sonic hedgehog in the ventral midline of the CNS.
Development. 2000 Sep; 127(18):3889-97.D

Abstract

The secreted molecule Sonic hedgehog (Shh) is crucial for floor plate and ventral brain development in amniote embryos. In zebrafish, mutations in cyclops (cyc), a gene that encodes a distinct signal related to the TGF(beta) family member Nodal, result in neural tube defects similar to those of shh null mice. cyc mutant embryos display cyclopia and lack floor plate and ventral brain regions, suggesting a role for Cyc in specification of these structures. cyc mutants express shh in the notochord but lack expression of shh in the ventral brain. Here we show that Cyc signalling can act directly on shh expression in neural tissue. Modulation of the Cyc signalling pathway by constitutive activation or inhibition of Smad2 leads to altered shh expression in zebrafish embryos. Ectopic activation of the shh promoter occurs in response to expression of Cyc signal transducers in the chick neural tube. Furthermore an enhancer of the shh gene, which controls ventral neural tube expression, is responsive to Cyc signal transducers. Our data imply that the Nodal related signal Cyc induces shh expression in the ventral neural tube. Based on the differential responsiveness of shh and other neural tube specific genes to Hedgehog and Cyc signalling, a two-step model for the establishment of the ventral midline of the CNS is proposed.

Authors+Show Affiliations

Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP 163, CU de Strasbourg, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10952887

Citation

Müller, F, et al. "Direct Action of the Nodal-related Signal Cyclops in Induction of Sonic Hedgehog in the Ventral Midline of the CNS." Development (Cambridge, England), vol. 127, no. 18, 2000, pp. 3889-97.
Müller F, Albert S, Blader P, et al. Direct action of the nodal-related signal cyclops in induction of sonic hedgehog in the ventral midline of the CNS. Development. 2000;127(18):3889-97.
Müller, F., Albert, S., Blader, P., Fischer, N., Hallonet, M., & Strähle, U. (2000). Direct action of the nodal-related signal cyclops in induction of sonic hedgehog in the ventral midline of the CNS. Development (Cambridge, England), 127(18), 3889-97.
Müller F, et al. Direct Action of the Nodal-related Signal Cyclops in Induction of Sonic Hedgehog in the Ventral Midline of the CNS. Development. 2000;127(18):3889-97. PubMed PMID: 10952887.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct action of the nodal-related signal cyclops in induction of sonic hedgehog in the ventral midline of the CNS. AU - Müller,F, AU - Albert,S, AU - Blader,P, AU - Fischer,N, AU - Hallonet,M, AU - Strähle,U, PY - 2000/8/23/pubmed PY - 2001/2/28/medline PY - 2000/8/23/entrez SP - 3889 EP - 97 JF - Development (Cambridge, England) JO - Development VL - 127 IS - 18 N2 - The secreted molecule Sonic hedgehog (Shh) is crucial for floor plate and ventral brain development in amniote embryos. In zebrafish, mutations in cyclops (cyc), a gene that encodes a distinct signal related to the TGF(beta) family member Nodal, result in neural tube defects similar to those of shh null mice. cyc mutant embryos display cyclopia and lack floor plate and ventral brain regions, suggesting a role for Cyc in specification of these structures. cyc mutants express shh in the notochord but lack expression of shh in the ventral brain. Here we show that Cyc signalling can act directly on shh expression in neural tissue. Modulation of the Cyc signalling pathway by constitutive activation or inhibition of Smad2 leads to altered shh expression in zebrafish embryos. Ectopic activation of the shh promoter occurs in response to expression of Cyc signal transducers in the chick neural tube. Furthermore an enhancer of the shh gene, which controls ventral neural tube expression, is responsive to Cyc signal transducers. Our data imply that the Nodal related signal Cyc induces shh expression in the ventral neural tube. Based on the differential responsiveness of shh and other neural tube specific genes to Hedgehog and Cyc signalling, a two-step model for the establishment of the ventral midline of the CNS is proposed. SN - 0950-1991 UR - https://www.unboundmedicine.com/medline/citation/10952887/Direct_action_of_the_nodal_related_signal_cyclops_in_induction_of_sonic_hedgehog_in_the_ventral_midline_of_the_CNS_ L2 - http://zfin.org/action/publication/view?accession=10952887 DB - PRIME DP - Unbound Medicine ER -