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Combined nasal administration of encephalitogenic myelin basic protein peptide 68-86 and IL-10 suppressed incipient experimental allergic encephalomyelitis in Lewis rats.
Clin Immunol. 2000 Sep; 96(3):205-11.CI

Abstract

Mucosal administration of low doses of myelin basic protein (MBP) peptide 68-86 (MBP 68-86) or anti-inflammatory cytokine IL-10 effectively prevented experimental allergic encephalomyelitis (EAE), but failed to suppress the disease if given after 7 days postimmunization (p.i.), i.e., after T cell priming had occurred. We anticipated that combined administration of autoantigen and IL-10 can treat incipient EAE. Lewis rats with EAE actively induced with MBP 68-86 and complete Freund's adjuvant received 120 microg MBP 68-86 + 200 ng IL-10 per rat per day from day 7 p.i. and for 5 consecutive days. These rats showed later onset, lower clinical scores, less body weight loss, and shorter duration of EAE than rats receiving MBP 68-86 or IL-10 only or PBS. EAE amelioration was associated with decreased infiltration of ED1(+) macrophages and CD4(+) T cells within the central nervous system and with decreased proliferative responses of lymph node cells, indicating that combined administration of MBP 68-86 and IL-10 induced immune hyporesponsiveness. IFN-gamma secretion as well as IFN-gamma, TNF-alpha, IL-4, and IL-10 mRNA expression by lymph node MNC was down-regulated in the treated rats. Immune hyporesponsiveness, rather than immune deviation or regulatory mechanisms, seems to be responsible for the protection of EAE after autoantigen + IL-10 administration by the nasal route.

Authors+Show Affiliations

Experimental Neurobiology Unit and Neuroimmunology Unit, Karolinska Institute, Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10964538

Citation

Xu, L Y., et al. "Combined Nasal Administration of Encephalitogenic Myelin Basic Protein Peptide 68-86 and IL-10 Suppressed Incipient Experimental Allergic Encephalomyelitis in Lewis Rats." Clinical Immunology (Orlando, Fla.), vol. 96, no. 3, 2000, pp. 205-11.
Xu LY, Yang JS, Huang YM, et al. Combined nasal administration of encephalitogenic myelin basic protein peptide 68-86 and IL-10 suppressed incipient experimental allergic encephalomyelitis in Lewis rats. Clin Immunol. 2000;96(3):205-11.
Xu, L. Y., Yang, J. S., Huang, Y. M., Levi, M., Link, H., & Xiao, B. G. (2000). Combined nasal administration of encephalitogenic myelin basic protein peptide 68-86 and IL-10 suppressed incipient experimental allergic encephalomyelitis in Lewis rats. Clinical Immunology (Orlando, Fla.), 96(3), 205-11.
Xu LY, et al. Combined Nasal Administration of Encephalitogenic Myelin Basic Protein Peptide 68-86 and IL-10 Suppressed Incipient Experimental Allergic Encephalomyelitis in Lewis Rats. Clin Immunol. 2000;96(3):205-11. PubMed PMID: 10964538.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined nasal administration of encephalitogenic myelin basic protein peptide 68-86 and IL-10 suppressed incipient experimental allergic encephalomyelitis in Lewis rats. AU - Xu,L Y, AU - Yang,J S, AU - Huang,Y M, AU - Levi,M, AU - Link,H, AU - Xiao,B G, PY - 2000/8/31/pubmed PY - 2000/9/30/medline PY - 2000/8/31/entrez SP - 205 EP - 11 JF - Clinical immunology (Orlando, Fla.) JO - Clin. Immunol. VL - 96 IS - 3 N2 - Mucosal administration of low doses of myelin basic protein (MBP) peptide 68-86 (MBP 68-86) or anti-inflammatory cytokine IL-10 effectively prevented experimental allergic encephalomyelitis (EAE), but failed to suppress the disease if given after 7 days postimmunization (p.i.), i.e., after T cell priming had occurred. We anticipated that combined administration of autoantigen and IL-10 can treat incipient EAE. Lewis rats with EAE actively induced with MBP 68-86 and complete Freund's adjuvant received 120 microg MBP 68-86 + 200 ng IL-10 per rat per day from day 7 p.i. and for 5 consecutive days. These rats showed later onset, lower clinical scores, less body weight loss, and shorter duration of EAE than rats receiving MBP 68-86 or IL-10 only or PBS. EAE amelioration was associated with decreased infiltration of ED1(+) macrophages and CD4(+) T cells within the central nervous system and with decreased proliferative responses of lymph node cells, indicating that combined administration of MBP 68-86 and IL-10 induced immune hyporesponsiveness. IFN-gamma secretion as well as IFN-gamma, TNF-alpha, IL-4, and IL-10 mRNA expression by lymph node MNC was down-regulated in the treated rats. Immune hyporesponsiveness, rather than immune deviation or regulatory mechanisms, seems to be responsible for the protection of EAE after autoantigen + IL-10 administration by the nasal route. SN - 1521-6616 UR - https://www.unboundmedicine.com/medline/citation/10964538/Combined_nasal_administration_of_encephalitogenic_myelin_basic_protein_peptide_68_86_and_IL_10_suppressed_incipient_experimental_allergic_encephalomyelitis_in_Lewis_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1521-6616(00)94895-2 DB - PRIME DP - Unbound Medicine ER -