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Development of brain injury in mice by Angiostrongylus cantonensis infection is associated with the induction of transcription factor NF-kappaB, nuclear protooncogenes, and protein tyrosine phosphorylation.
Exp Parasitol. 2000 Jul; 95(3):202-8.EP

Abstract

Eosinophilic meningitis or meningoencephalitis caused by Angiostrongylus cantonensis is endemic to the Pacific area of Asia, especially Taiwan, Thailand, and Japan. Although eosinophilia is an important clinical manifestation of A. cantonensis infection, the role of eosinophils in the progress of the infection remains to be elucidated. In this experiment, we showed that A. cantonensis-caused eosinoplia and inflammation might lead to the induction of NF-kappaB and protooncogene expression via activation of the tyrosine phosphorylation signal pathway. After mice were infected daily with 30 third-stage larvae of A. cantonensis by oral adminstration for 6 weeks, no significant differences PKC-alpha, MEK-1, ERK-2, JNK, and p38 protein expression were found between the control and infected mice. However, the protein tyrosine phosphorylation levels, NF-kappaB, and iNOS protein products were significantly increased by 3.5-, 3.3-, and 6.3-fold, respectively, after 3 weeks of A. cantonensis infection. The same pattern was found for c-Myc, c-Jun, and c-Fos proteins, which were elevated by 3.2-, 2.3-, and 3.4-fold, respectively, compared to control animals after 3 weeks. The expression potency of these proteins started increasing in week 1, reaching maximal induction in week 3, and then declining in week 5 after A. cantonensis infection. Another consistent result was noted in the pathological observations, including eosinophilia, leukocyte infiltration, granulomatous reactions, and time responses in brain tissues of infected mice. These data suggest that the development of brain injury by eosinophlia of A. cantonensis infection is associated with NF-kappaB and/or nuclear protooncogenes expression, which is activated by the tyrosine phosphorylation pathway.

Authors+Show Affiliations

Department of Parasitology. Chung SHan Medical and Dental College, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10964648

Citation

Lee, H H., et al. "Development of Brain Injury in Mice By Angiostrongylus Cantonensis Infection Is Associated With the Induction of Transcription Factor NF-kappaB, Nuclear Protooncogenes, and Protein Tyrosine Phosphorylation." Experimental Parasitology, vol. 95, no. 3, 2000, pp. 202-8.
Lee HH, Shiow SJ, Chung HC, et al. Development of brain injury in mice by Angiostrongylus cantonensis infection is associated with the induction of transcription factor NF-kappaB, nuclear protooncogenes, and protein tyrosine phosphorylation. Exp Parasitol. 2000;95(3):202-8.
Lee, H. H., Shiow, S. J., Chung, H. C., Huang, C. Y., Lin, C. L., Hsu, J. D., Shyu, L. Y., & Wang, C. J. (2000). Development of brain injury in mice by Angiostrongylus cantonensis infection is associated with the induction of transcription factor NF-kappaB, nuclear protooncogenes, and protein tyrosine phosphorylation. Experimental Parasitology, 95(3), 202-8.
Lee HH, et al. Development of Brain Injury in Mice By Angiostrongylus Cantonensis Infection Is Associated With the Induction of Transcription Factor NF-kappaB, Nuclear Protooncogenes, and Protein Tyrosine Phosphorylation. Exp Parasitol. 2000;95(3):202-8. PubMed PMID: 10964648.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of brain injury in mice by Angiostrongylus cantonensis infection is associated with the induction of transcription factor NF-kappaB, nuclear protooncogenes, and protein tyrosine phosphorylation. AU - Lee,H H, AU - Shiow,S J, AU - Chung,H C, AU - Huang,C Y, AU - Lin,C L, AU - Hsu,J D, AU - Shyu,L Y, AU - Wang,C J, PY - 2000/8/31/pubmed PY - 2000/9/30/medline PY - 2000/8/31/entrez SP - 202 EP - 8 JF - Experimental parasitology JO - Exp Parasitol VL - 95 IS - 3 N2 - Eosinophilic meningitis or meningoencephalitis caused by Angiostrongylus cantonensis is endemic to the Pacific area of Asia, especially Taiwan, Thailand, and Japan. Although eosinophilia is an important clinical manifestation of A. cantonensis infection, the role of eosinophils in the progress of the infection remains to be elucidated. In this experiment, we showed that A. cantonensis-caused eosinoplia and inflammation might lead to the induction of NF-kappaB and protooncogene expression via activation of the tyrosine phosphorylation signal pathway. After mice were infected daily with 30 third-stage larvae of A. cantonensis by oral adminstration for 6 weeks, no significant differences PKC-alpha, MEK-1, ERK-2, JNK, and p38 protein expression were found between the control and infected mice. However, the protein tyrosine phosphorylation levels, NF-kappaB, and iNOS protein products were significantly increased by 3.5-, 3.3-, and 6.3-fold, respectively, after 3 weeks of A. cantonensis infection. The same pattern was found for c-Myc, c-Jun, and c-Fos proteins, which were elevated by 3.2-, 2.3-, and 3.4-fold, respectively, compared to control animals after 3 weeks. The expression potency of these proteins started increasing in week 1, reaching maximal induction in week 3, and then declining in week 5 after A. cantonensis infection. Another consistent result was noted in the pathological observations, including eosinophilia, leukocyte infiltration, granulomatous reactions, and time responses in brain tissues of infected mice. These data suggest that the development of brain injury by eosinophlia of A. cantonensis infection is associated with NF-kappaB and/or nuclear protooncogenes expression, which is activated by the tyrosine phosphorylation pathway. SN - 0014-4894 UR - https://www.unboundmedicine.com/medline/citation/10964648/Development_of_brain_injury_in_mice_by_Angiostrongylus_cantonensis_infection_is_associated_with_the_induction_of_transcription_factor_NF_kappaB_nuclear_protooncogenes_and_protein_tyrosine_phosphorylation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4894(00)94530-2 DB - PRIME DP - Unbound Medicine ER -