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HuR, a protein implicated in oncogene and growth factor mRNA decay, binds to the 3' ends of hepatitis C virus RNA of both polarities.
Virology. 2000 Sep 01; 274(2):378-90.V

Abstract

To identify cellular factors that interact with hepatitis C virus RNA, cellular extracts were subjected to UV cross-linking to radiolabeled RNAs corresponding to the hepatitis C virus 5' and 3' untranslated regions of positive and negative polarities. Our results demonstrate that the U-rich region of the hepatitis C virus 3' untranslated region of the positive RNA strand is a hot spot for cellular RNA binding proteins. Two of these proteins were identified as the ELAV-like HuR protein and hnRNP C. Interestingly, HuR and hnRNP C also interacted with the 3' end of the RNA representing the negative strand of the HCV genome. The binding of HuR and hnRNP C to the 3' ends of the HCV RNAs of both negative and positive polarities suggests that HuR and hnRNP C may be involved in the transcription of the HCV RNA genome. Alternatively, they act by protecting the HCV RNAs from premature degradation by binding to their 3' ends. However, we were unable to demonstrate an effect on HCV RNA stability by the HuR protein. These interactions may be necessary for the establishment of chronic active infections that may develop into cirrhosis or hepatocellular carcinoma.

Authors+Show Affiliations

Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, 751 23, Sweden.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10964780

Citation

Spångberg, K, et al. "HuR, a Protein Implicated in Oncogene and Growth Factor mRNA Decay, Binds to the 3' Ends of Hepatitis C Virus RNA of Both Polarities." Virology, vol. 274, no. 2, 2000, pp. 378-90.
Spångberg K, Wiklund L, Schwartz S. HuR, a protein implicated in oncogene and growth factor mRNA decay, binds to the 3' ends of hepatitis C virus RNA of both polarities. Virology. 2000;274(2):378-90.
Spångberg, K., Wiklund, L., & Schwartz, S. (2000). HuR, a protein implicated in oncogene and growth factor mRNA decay, binds to the 3' ends of hepatitis C virus RNA of both polarities. Virology, 274(2), 378-90.
Spångberg K, Wiklund L, Schwartz S. HuR, a Protein Implicated in Oncogene and Growth Factor mRNA Decay, Binds to the 3' Ends of Hepatitis C Virus RNA of Both Polarities. Virology. 2000 Sep 1;274(2):378-90. PubMed PMID: 10964780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HuR, a protein implicated in oncogene and growth factor mRNA decay, binds to the 3' ends of hepatitis C virus RNA of both polarities. AU - Spångberg,K, AU - Wiklund,L, AU - Schwartz,S, PY - 2000/8/31/pubmed PY - 2000/9/30/medline PY - 2000/8/31/entrez SP - 378 EP - 90 JF - Virology JO - Virology VL - 274 IS - 2 N2 - To identify cellular factors that interact with hepatitis C virus RNA, cellular extracts were subjected to UV cross-linking to radiolabeled RNAs corresponding to the hepatitis C virus 5' and 3' untranslated regions of positive and negative polarities. Our results demonstrate that the U-rich region of the hepatitis C virus 3' untranslated region of the positive RNA strand is a hot spot for cellular RNA binding proteins. Two of these proteins were identified as the ELAV-like HuR protein and hnRNP C. Interestingly, HuR and hnRNP C also interacted with the 3' end of the RNA representing the negative strand of the HCV genome. The binding of HuR and hnRNP C to the 3' ends of the HCV RNAs of both negative and positive polarities suggests that HuR and hnRNP C may be involved in the transcription of the HCV RNA genome. Alternatively, they act by protecting the HCV RNAs from premature degradation by binding to their 3' ends. However, we were unable to demonstrate an effect on HCV RNA stability by the HuR protein. These interactions may be necessary for the establishment of chronic active infections that may develop into cirrhosis or hepatocellular carcinoma. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/10964780/HuR_a_protein_implicated_in_oncogene_and_growth_factor_mRNA_decay_binds_to_the_3'_ends_of_hepatitis_C_virus_RNA_of_both_polarities_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(00)90461-6 DB - PRIME DP - Unbound Medicine ER -