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Inverse mRNA expression of the selenocysteine-containing proteins GI-GPx and SeP in colorectal adenomas compared with adjacent normal mucosa.
Nutr Cancer 2000; 37(1):108-16NC

Abstract

Four selenocysteine-containing proteins (gastrointestinal glutathione peroxidase, plasma glutathione peroxidase, selenoprotein P, and thioredoxin reductase-alpha) are expressed in the colonic mucosa. Because of their antioxidant functions, a protective role in colon carcinogenesis is discussed. The aim of this study was to elucidate an involvement of gastrointestinal selenoproteins during the adenoma-carcinoma sequence. Matched pairs of biopsies of colorectal adenomas and adjacent normal mucosa from 11 patients were analyzed for mRNA expression, protein expression, or enzyme activity of selenoproteins by Northern blot, Western blot, or enzymatic tests. All adenomas revealed a marked reduction of selenoprotein P and a variable increase of gastrointestinal glutathione peroxidase mRNA compared with adjacent tissue. Thioredoxin reductase-alpha and plasma glutathione peroxidase mRNA expression were not altered in adenomas. The Northern blot results were confirmed by Western blot analysis or enzyme activity measurement, respectively. We conclude that gastrointestinal glutathione peroxidase and selenoprotein P play a complementary role in the antioxidative cell defense along the adenoma-carcinoma sequence. It remains to be shown whether upregulation of gastrointestinal glutathione peroxidase in adenomas represents a compensatory mechanism to reduce susceptibility for oxidative damage resulting from the loss of selenoprotein P.

Authors+Show Affiliations

Medizinische Poliklinik, University of Würzburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10965527

Citation

Mörk, H, et al. "Inverse mRNA Expression of the Selenocysteine-containing Proteins GI-GPx and SeP in Colorectal Adenomas Compared With Adjacent Normal Mucosa." Nutrition and Cancer, vol. 37, no. 1, 2000, pp. 108-16.
Mörk H, al-Taie OH, Bähr K, et al. Inverse mRNA expression of the selenocysteine-containing proteins GI-GPx and SeP in colorectal adenomas compared with adjacent normal mucosa. Nutr Cancer. 2000;37(1):108-16.
Mörk, H., al-Taie, O. H., Bähr, K., Zierer, A., Beck, C., Scheurlen, M., ... Köhrle, J. (2000). Inverse mRNA expression of the selenocysteine-containing proteins GI-GPx and SeP in colorectal adenomas compared with adjacent normal mucosa. Nutrition and Cancer, 37(1), pp. 108-16.
Mörk H, et al. Inverse mRNA Expression of the Selenocysteine-containing Proteins GI-GPx and SeP in Colorectal Adenomas Compared With Adjacent Normal Mucosa. Nutr Cancer. 2000;37(1):108-16. PubMed PMID: 10965527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inverse mRNA expression of the selenocysteine-containing proteins GI-GPx and SeP in colorectal adenomas compared with adjacent normal mucosa. AU - Mörk,H, AU - al-Taie,O H, AU - Bähr,K, AU - Zierer,A, AU - Beck,C, AU - Scheurlen,M, AU - Jakob,F, AU - Köhrle,J, PY - 2000/8/31/pubmed PY - 2001/6/2/medline PY - 2000/8/31/entrez SP - 108 EP - 16 JF - Nutrition and cancer JO - Nutr Cancer VL - 37 IS - 1 N2 - Four selenocysteine-containing proteins (gastrointestinal glutathione peroxidase, plasma glutathione peroxidase, selenoprotein P, and thioredoxin reductase-alpha) are expressed in the colonic mucosa. Because of their antioxidant functions, a protective role in colon carcinogenesis is discussed. The aim of this study was to elucidate an involvement of gastrointestinal selenoproteins during the adenoma-carcinoma sequence. Matched pairs of biopsies of colorectal adenomas and adjacent normal mucosa from 11 patients were analyzed for mRNA expression, protein expression, or enzyme activity of selenoproteins by Northern blot, Western blot, or enzymatic tests. All adenomas revealed a marked reduction of selenoprotein P and a variable increase of gastrointestinal glutathione peroxidase mRNA compared with adjacent tissue. Thioredoxin reductase-alpha and plasma glutathione peroxidase mRNA expression were not altered in adenomas. The Northern blot results were confirmed by Western blot analysis or enzyme activity measurement, respectively. We conclude that gastrointestinal glutathione peroxidase and selenoprotein P play a complementary role in the antioxidative cell defense along the adenoma-carcinoma sequence. It remains to be shown whether upregulation of gastrointestinal glutathione peroxidase in adenomas represents a compensatory mechanism to reduce susceptibility for oxidative damage resulting from the loss of selenoprotein P. SN - 0163-5581 UR - https://www.unboundmedicine.com/medline/citation/10965527/Inverse_mRNA_expression_of_the_selenocysteine_containing_proteins_GI_GPx_and_SeP_in_colorectal_adenomas_compared_with_adjacent_normal_mucosa_ L2 - http://www.tandfonline.com/doi/full/10.1207/S15327914NC3701_14 DB - PRIME DP - Unbound Medicine ER -