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Identification of osteoblast/osteocyte factor 45 (OF45), a bone-specific cDNA encoding an RGD-containing protein that is highly expressed in osteoblasts and osteocytes.
J Biol Chem. 2000 Nov 17; 275(46):36172-80.JB

Abstract

We describe the cloning and characterization of a novel bone-specific cDNA predicted to encode an extracellular matrix protein. This cDNA was identified by subtractive hybridization based upon its high expression in bone marrow-derived osteoblasts. By Northern blot analysis, we detected a single 2-kilobase mRNA transcript in bone, whereas no expression was detected in other tissues. Immunohistochemistry revealed that the protein was expressed highly in osteocytes within trabecular and cortical bone. RNA and protein expression analysis using in vivo marrow ablation as a model of bone remodeling demonstrated that this gene was expressed only in cells that were embedded within bone matrix in contrast to the earlier expression of known osteoblast markers. The cDNA was predicted to encode a serine/glycine-rich secreted peptide containing numerous potential phosphorylation sites and one RGD sequence motif. The interaction of RGD domain-containing peptides with integrins has been shown previously to regulate bone remodeling by promoting recruitment, attachment, and differentiation of osteoblasts and osteoclasts. Secretion of this RGD-containing protein from osteocytes has the potential to regulate cellular activities within the bone environment and thereby may impact bone homeostasis. We propose the name OF45 (osteoblast/osteocyte factor of 45 kDa) for this novel cDNA.

Authors+Show Affiliations

Department of Cardiovascular & Metabolic Diseases, Global Research and Development, Pfizer, Inc., Groton, Connecticut 06340, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10967096

Citation

Petersen, D N., et al. "Identification of Osteoblast/osteocyte Factor 45 (OF45), a Bone-specific cDNA Encoding an RGD-containing Protein That Is Highly Expressed in Osteoblasts and Osteocytes." The Journal of Biological Chemistry, vol. 275, no. 46, 2000, pp. 36172-80.
Petersen DN, Tkalcevic GT, Mansolf AL, et al. Identification of osteoblast/osteocyte factor 45 (OF45), a bone-specific cDNA encoding an RGD-containing protein that is highly expressed in osteoblasts and osteocytes. J Biol Chem. 2000;275(46):36172-80.
Petersen, D. N., Tkalcevic, G. T., Mansolf, A. L., Rivera-Gonzalez, R., & Brown, T. A. (2000). Identification of osteoblast/osteocyte factor 45 (OF45), a bone-specific cDNA encoding an RGD-containing protein that is highly expressed in osteoblasts and osteocytes. The Journal of Biological Chemistry, 275(46), 36172-80.
Petersen DN, et al. Identification of Osteoblast/osteocyte Factor 45 (OF45), a Bone-specific cDNA Encoding an RGD-containing Protein That Is Highly Expressed in Osteoblasts and Osteocytes. J Biol Chem. 2000 Nov 17;275(46):36172-80. PubMed PMID: 10967096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of osteoblast/osteocyte factor 45 (OF45), a bone-specific cDNA encoding an RGD-containing protein that is highly expressed in osteoblasts and osteocytes. AU - Petersen,D N, AU - Tkalcevic,G T, AU - Mansolf,A L, AU - Rivera-Gonzalez,R, AU - Brown,T A, PY - 2000/9/1/pubmed PY - 2001/2/28/medline PY - 2000/9/1/entrez SP - 36172 EP - 80 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 275 IS - 46 N2 - We describe the cloning and characterization of a novel bone-specific cDNA predicted to encode an extracellular matrix protein. This cDNA was identified by subtractive hybridization based upon its high expression in bone marrow-derived osteoblasts. By Northern blot analysis, we detected a single 2-kilobase mRNA transcript in bone, whereas no expression was detected in other tissues. Immunohistochemistry revealed that the protein was expressed highly in osteocytes within trabecular and cortical bone. RNA and protein expression analysis using in vivo marrow ablation as a model of bone remodeling demonstrated that this gene was expressed only in cells that were embedded within bone matrix in contrast to the earlier expression of known osteoblast markers. The cDNA was predicted to encode a serine/glycine-rich secreted peptide containing numerous potential phosphorylation sites and one RGD sequence motif. The interaction of RGD domain-containing peptides with integrins has been shown previously to regulate bone remodeling by promoting recruitment, attachment, and differentiation of osteoblasts and osteoclasts. Secretion of this RGD-containing protein from osteocytes has the potential to regulate cellular activities within the bone environment and thereby may impact bone homeostasis. We propose the name OF45 (osteoblast/osteocyte factor of 45 kDa) for this novel cDNA. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/10967096/Identification_of_osteoblast/osteocyte_factor_45__OF45__a_bone_specific_cDNA_encoding_an_RGD_containing_protein_that_is_highly_expressed_in_osteoblasts_and_osteocytes_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=10967096 DB - PRIME DP - Unbound Medicine ER -