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Time-dependent changes in superoxide dismutase, catalase, xanthine dehydrogenase and oxidase activities in focal cerebral ischaemia.
Cytobios. 2000; 102(401):157-72.C

Abstract

Time-dependent changes in the activities of antioxidant enzymes and an oxidant enzyme, xanthine oxidase (XO), were detected in primary and peri-ischaemic brain regions during permanent occlusion of the middle cerebral artery (MCAO) in rats. There were no changes in superoxide dismutase (SOD) and catalase (CAT) activities after 3 h of MCAO, whereas antioxidant enzyme activities decreased significantly in ischaemic brain areas following 24 h of ischaemia. After 48 h, the enzyme activities returned to the baseline but then a further increase was observed in ischaemic brain areas by 72 h post-ischaemia. Normally, XO exists as a dehydrogenase (XD), but it is converted to XO which contributes to injury in some ischaemic tissues. The XO activity increased slightly at 3 h after ischaemia, but after 24 h of ischaemia it returned to the baseline and then remained relatively unchanged in ischaemic areas. Pretreatment with allopurinol before ischaemia prevented changes in SOD and CAT activities and attenuated brain oedema during 24 h of ischaemia. Neither XO nor XD activity changed in allopurinol-treated rats at the times of ischaemia. These results indicated that ischaemic brain tissue remained vulnerable to free radical damage for as long as 48 h after ischaemia, and XO was probably not an important source of free radicals in cerebral ischaemia.

Authors+Show Affiliations

Department of Neurology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

10969880

Citation

Sermet, A, et al. "Time-dependent Changes in Superoxide Dismutase, Catalase, Xanthine Dehydrogenase and Oxidase Activities in Focal Cerebral Ischaemia." Cytobios, vol. 102, no. 401, 2000, pp. 157-72.
Sermet A, Taşdemir N, Deniz B, et al. Time-dependent changes in superoxide dismutase, catalase, xanthine dehydrogenase and oxidase activities in focal cerebral ischaemia. Cytobios. 2000;102(401):157-72.
Sermet, A., Taşdemir, N., Deniz, B., & Atmaca, M. (2000). Time-dependent changes in superoxide dismutase, catalase, xanthine dehydrogenase and oxidase activities in focal cerebral ischaemia. Cytobios, 102(401), 157-72.
Sermet A, et al. Time-dependent Changes in Superoxide Dismutase, Catalase, Xanthine Dehydrogenase and Oxidase Activities in Focal Cerebral Ischaemia. Cytobios. 2000;102(401):157-72. PubMed PMID: 10969880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Time-dependent changes in superoxide dismutase, catalase, xanthine dehydrogenase and oxidase activities in focal cerebral ischaemia. AU - Sermet,A, AU - Taşdemir,N, AU - Deniz,B, AU - Atmaca,M, PY - 2000/9/2/pubmed PY - 2001/2/28/medline PY - 2000/9/2/entrez SP - 157 EP - 72 JF - Cytobios JO - Cytobios VL - 102 IS - 401 N2 - Time-dependent changes in the activities of antioxidant enzymes and an oxidant enzyme, xanthine oxidase (XO), were detected in primary and peri-ischaemic brain regions during permanent occlusion of the middle cerebral artery (MCAO) in rats. There were no changes in superoxide dismutase (SOD) and catalase (CAT) activities after 3 h of MCAO, whereas antioxidant enzyme activities decreased significantly in ischaemic brain areas following 24 h of ischaemia. After 48 h, the enzyme activities returned to the baseline but then a further increase was observed in ischaemic brain areas by 72 h post-ischaemia. Normally, XO exists as a dehydrogenase (XD), but it is converted to XO which contributes to injury in some ischaemic tissues. The XO activity increased slightly at 3 h after ischaemia, but after 24 h of ischaemia it returned to the baseline and then remained relatively unchanged in ischaemic areas. Pretreatment with allopurinol before ischaemia prevented changes in SOD and CAT activities and attenuated brain oedema during 24 h of ischaemia. Neither XO nor XD activity changed in allopurinol-treated rats at the times of ischaemia. These results indicated that ischaemic brain tissue remained vulnerable to free radical damage for as long as 48 h after ischaemia, and XO was probably not an important source of free radicals in cerebral ischaemia. SN - 0011-4529 UR - https://www.unboundmedicine.com/medline/citation/10969880/Time_dependent_changes_in_superoxide_dismutase_catalase_xanthine_dehydrogenase_and_oxidase_activities_in_focal_cerebral_ischaemia_ DB - PRIME DP - Unbound Medicine ER -