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Distinct myosin heavy chain isoform transitions in developing slow and fast cat hindlimb muscles.
Cells Tissues Organs. 2000; 167(2-3):138-52.CT

Abstract

The expression of myosin heavy chain (MHC) isoforms leading to adult fiber phenotypes in the tibialis anterior (TA) and soleus muscles of the cat were investigated from embryonic day 35 to 1 year after birth. Electrophoresis and immunoblotting of myofibrils demonstrated the expression of 5 different MHC isoforms, i.e. I, IIa, IIx, embryonic, and neonatal, during development. Based on electrophoresis, the adult-like MHC composition of the soleus and TA were not observed until postnatal day 40 (P40) and 120 (P120), respectively. In contrast, immunohistochemical analyses revealed that the adult-like fiber phenotype composition was attained much later (P120) in the soleus. The existence of multiple MHC isoforms in individual fibers suggested that transitions occurred until P120 in both muscles. Adult type I fibers were first observed at P1. Adult IIA fibers were first observed at P30 in the TA and P40 in the soleus. IIX fibers were not identified until P40 in the TA. The transition to the predominantly slow phenotype of the soleus involved a gradual loss of embryonic and fast isoforms accompanied by an accumulation of slow MHC. In contrast, the expression of slow and fast MHC in the fast TA muscle was relatively unchanged throughout development. These results show that the establishment of a given MHC-based fiber phenotype varies significantly between slow and fast muscles in the kitten.

Authors+Show Affiliations

Department of Physiological Science, University of California, Los Angeles, CA 90095-1761, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10971038

Citation

Unguez, G A., et al. "Distinct Myosin Heavy Chain Isoform Transitions in Developing Slow and Fast Cat Hindlimb Muscles." Cells, Tissues, Organs, vol. 167, no. 2-3, 2000, pp. 138-52.
Unguez GA, Talmadge RJ, Roy RR, et al. Distinct myosin heavy chain isoform transitions in developing slow and fast cat hindlimb muscles. Cells Tissues Organs. 2000;167(2-3):138-52.
Unguez, G. A., Talmadge, R. J., Roy, R. R., Dalponte, D., & Edgerton, V. R. (2000). Distinct myosin heavy chain isoform transitions in developing slow and fast cat hindlimb muscles. Cells, Tissues, Organs, 167(2-3), 138-52.
Unguez GA, et al. Distinct Myosin Heavy Chain Isoform Transitions in Developing Slow and Fast Cat Hindlimb Muscles. Cells Tissues Organs. 2000;167(2-3):138-52. PubMed PMID: 10971038.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distinct myosin heavy chain isoform transitions in developing slow and fast cat hindlimb muscles. AU - Unguez,G A, AU - Talmadge,R J, AU - Roy,R R, AU - Dalponte,D, AU - Edgerton,V R, PY - 2000/9/6/pubmed PY - 2000/9/30/medline PY - 2000/9/6/entrez KW - Non-programmatic SP - 138 EP - 52 JF - Cells, tissues, organs JO - Cells Tissues Organs VL - 167 IS - 2-3 N2 - The expression of myosin heavy chain (MHC) isoforms leading to adult fiber phenotypes in the tibialis anterior (TA) and soleus muscles of the cat were investigated from embryonic day 35 to 1 year after birth. Electrophoresis and immunoblotting of myofibrils demonstrated the expression of 5 different MHC isoforms, i.e. I, IIa, IIx, embryonic, and neonatal, during development. Based on electrophoresis, the adult-like MHC composition of the soleus and TA were not observed until postnatal day 40 (P40) and 120 (P120), respectively. In contrast, immunohistochemical analyses revealed that the adult-like fiber phenotype composition was attained much later (P120) in the soleus. The existence of multiple MHC isoforms in individual fibers suggested that transitions occurred until P120 in both muscles. Adult type I fibers were first observed at P1. Adult IIA fibers were first observed at P30 in the TA and P40 in the soleus. IIX fibers were not identified until P40 in the TA. The transition to the predominantly slow phenotype of the soleus involved a gradual loss of embryonic and fast isoforms accompanied by an accumulation of slow MHC. In contrast, the expression of slow and fast MHC in the fast TA muscle was relatively unchanged throughout development. These results show that the establishment of a given MHC-based fiber phenotype varies significantly between slow and fast muscles in the kitten. SN - 1422-6405 UR - https://www.unboundmedicine.com/medline/citation/10971038/Distinct_myosin_heavy_chain_isoform_transitions_in_developing_slow_and_fast_cat_hindlimb_muscles_ L2 - https://www.karger.com?DOI=10.1159/000016777 DB - PRIME DP - Unbound Medicine ER -