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Phase behaviour of skin barrier model membranes at pH 7.4.
Cell Mol Biol (Noisy-le-grand). 2000 Jul; 46(5):979-92.CM

Abstract

The main function of the skin is to protect the body against exogenous substances. The skin barrier is located in the outermost layer of the skin, the stratum corneum (SC). This layer consists of keratin enriched cells embedded in lipid lamellae that form the main barrier for diffusion of substances through the skin. The main lipid classes in this barrier are ceramides, cholesterol and free fatty acids. Cholesterol sulfate and calcium are also present in SC. Furthermore it has been suggested that a pH gradient exists. In a previous paper the effect of cholesterol sulfate and calcium on the lipid phase behaviour of mixtures prepared from cholesterol, ceramides and free fatty acids at pH 5 was reported (approximate pH at the skin surface). In the present study the phase behaviour of mixtures prepared from cholesterol, ceramides and free fatty acids prepared at pH 7.4 (the pH of viable cells) has been examined between 25 and 95 degrees C. Our studies reveal that a reversed hexagonal phase has been formed at elevated temperatures. Addition of calcium inhibits the formation of the reversed hexagonal phase, while cholesterol sulfate promotes the presence of the reversed hexagonal phase at increased temperatures. From our results we can conclude that the lipid mixtures prepared at pH 5 resemble more closely the lipid phase behaviour in intact SC than the lipid mixtures prepared at pH 7.4.

Authors+Show Affiliations

Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, The Netherlands. Bouwstra@chem.leidenUniv.nlNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

10976878

Citation

Bouwsta, J A., et al. "Phase Behaviour of Skin Barrier Model Membranes at pH 7.4." Cellular and Molecular Biology (Noisy-le-Grand, France), vol. 46, no. 5, 2000, pp. 979-92.
Bouwsta JA, Gooris GS, Dubbelaar FE, et al. Phase behaviour of skin barrier model membranes at pH 7.4. Cell Mol Biol (Noisy-le-grand). 2000;46(5):979-92.
Bouwsta, J. A., Gooris, G. S., Dubbelaar, F. E., & Ponec, M. (2000). Phase behaviour of skin barrier model membranes at pH 7.4. Cellular and Molecular Biology (Noisy-le-Grand, France), 46(5), 979-92.
Bouwsta JA, et al. Phase Behaviour of Skin Barrier Model Membranes at pH 7.4. Cell Mol Biol (Noisy-le-grand). 2000;46(5):979-92. PubMed PMID: 10976878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phase behaviour of skin barrier model membranes at pH 7.4. AU - Bouwsta,J A, AU - Gooris,G S, AU - Dubbelaar,F E, AU - Ponec,M, PY - 2000/9/8/pubmed PY - 2000/10/14/medline PY - 2000/9/8/entrez SP - 979 EP - 92 JF - Cellular and molecular biology (Noisy-le-Grand, France) JO - Cell Mol Biol (Noisy-le-grand) VL - 46 IS - 5 N2 - The main function of the skin is to protect the body against exogenous substances. The skin barrier is located in the outermost layer of the skin, the stratum corneum (SC). This layer consists of keratin enriched cells embedded in lipid lamellae that form the main barrier for diffusion of substances through the skin. The main lipid classes in this barrier are ceramides, cholesterol and free fatty acids. Cholesterol sulfate and calcium are also present in SC. Furthermore it has been suggested that a pH gradient exists. In a previous paper the effect of cholesterol sulfate and calcium on the lipid phase behaviour of mixtures prepared from cholesterol, ceramides and free fatty acids at pH 5 was reported (approximate pH at the skin surface). In the present study the phase behaviour of mixtures prepared from cholesterol, ceramides and free fatty acids prepared at pH 7.4 (the pH of viable cells) has been examined between 25 and 95 degrees C. Our studies reveal that a reversed hexagonal phase has been formed at elevated temperatures. Addition of calcium inhibits the formation of the reversed hexagonal phase, while cholesterol sulfate promotes the presence of the reversed hexagonal phase at increased temperatures. From our results we can conclude that the lipid mixtures prepared at pH 5 resemble more closely the lipid phase behaviour in intact SC than the lipid mixtures prepared at pH 7.4. SN - 0145-5680 UR - https://www.unboundmedicine.com/medline/citation/10976878/Phase_behaviour_of_skin_barrier_model_membranes_at_pH_7_4_ DB - PRIME DP - Unbound Medicine ER -