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Heterogenous immunohistochemical expression of microglia-specific ionized calcium binding adaptor protein (Iba1) in the mouse olfactory bulb.

Abstract

Glial cells regulate some neural functions which depend on the homeostatic maintenance of extracellular calcium within narrow physiological ranges. In this study, the presence of microglia-specific ionized calcium binding adaptor molecule 1 (Iba1) was examined in the mouse olfactory bulb. A heterogenous pattern of Iba1-positive cells expression was observed between the main and accessory olfactory bulbs (MOB and AOB, respectively). While Iba1 was almost uniformly expressed among the laminae of the MOB, its expression showed spatial variations from the anterior to the posterior regions of the AOB. Double immunofluorescence was used to confirm that Iba1 is not expressed in astrocytes which stained for glial fibrillary acidic protein. Since Iba1 may mediate calcium signals in microglia, the observations suggest a potential involvement of Iba1 and hence microglia in olfactory bulb function and/or homeostasis. Together with our previous observation, this provides further support of a bulbar neuron-glia system of potential physiological significance.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Physiology, Kochi Medical School, Okoh-cho, Nankoku, 783-8505, Kochi, Japan. okere@mcphu.edu

    Source

    Brain research 877:1 2000 Sep 15 pg 85-90

    MeSH

    Animals
    Calcium-Binding Proteins
    Female
    Immunohistochemistry
    Mice
    Mice, Inbred BALB C
    Microfilament Proteins
    Microglia
    Olfactory Bulb

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    10980247

    Citation

    Okere, C O., and H Kaba. "Heterogenous Immunohistochemical Expression of Microglia-specific Ionized Calcium Binding Adaptor Protein (Iba1) in the Mouse Olfactory Bulb." Brain Research, vol. 877, no. 1, 2000, pp. 85-90.
    Okere CO, Kaba H. Heterogenous immunohistochemical expression of microglia-specific ionized calcium binding adaptor protein (Iba1) in the mouse olfactory bulb. Brain Res. 2000;877(1):85-90.
    Okere, C. O., & Kaba, H. (2000). Heterogenous immunohistochemical expression of microglia-specific ionized calcium binding adaptor protein (Iba1) in the mouse olfactory bulb. Brain Research, 877(1), pp. 85-90.
    Okere CO, Kaba H. Heterogenous Immunohistochemical Expression of Microglia-specific Ionized Calcium Binding Adaptor Protein (Iba1) in the Mouse Olfactory Bulb. Brain Res. 2000 Sep 15;877(1):85-90. PubMed PMID: 10980247.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Heterogenous immunohistochemical expression of microglia-specific ionized calcium binding adaptor protein (Iba1) in the mouse olfactory bulb. AU - Okere,C O, AU - Kaba,H, PY - 2000/9/12/pubmed PY - 2001/2/28/medline PY - 2000/9/12/entrez SP - 85 EP - 90 JF - Brain research JO - Brain Res. VL - 877 IS - 1 N2 - Glial cells regulate some neural functions which depend on the homeostatic maintenance of extracellular calcium within narrow physiological ranges. In this study, the presence of microglia-specific ionized calcium binding adaptor molecule 1 (Iba1) was examined in the mouse olfactory bulb. A heterogenous pattern of Iba1-positive cells expression was observed between the main and accessory olfactory bulbs (MOB and AOB, respectively). While Iba1 was almost uniformly expressed among the laminae of the MOB, its expression showed spatial variations from the anterior to the posterior regions of the AOB. Double immunofluorescence was used to confirm that Iba1 is not expressed in astrocytes which stained for glial fibrillary acidic protein. Since Iba1 may mediate calcium signals in microglia, the observations suggest a potential involvement of Iba1 and hence microglia in olfactory bulb function and/or homeostasis. Together with our previous observation, this provides further support of a bulbar neuron-glia system of potential physiological significance. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/10980247/Heterogenous_immunohistochemical_expression_of_microglia_specific_ionized_calcium_binding_adaptor_protein__Iba1__in_the_mouse_olfactory_bulb_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(00)02656-1 DB - PRIME DP - Unbound Medicine ER -