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Neuroinvasion by human respiratory coronaviruses.
J Virol. 2000 Oct; 74(19):8913-21.JV

Abstract

Human coronaviruses (HCoV) cause common colds but can also infect neural cell cultures. To provide definitive experimental evidence for the neurotropism and neuroinvasion of HCoV and its possible association with multiple sclerosis (MS), we have performed an extensive search and characterization of HCoV RNA in a large panel of human brain autopsy samples. Very stringent reverse transcription-PCR with two primer pairs for both viral strains (229E and OC43), combined with Southern hybridization, was performed on samples from 90 coded donors with various neurological diseases (39 with MS and 26 with other neurological diseases) or normal controls (25 patients). We report that 44% (40 of 90) of donors were positive for 229E and that 23% (21 of 90) were positive for OC43. A statistically significant higher prevalence of OC43 in MS patients (35.9%; 14 of 39) than in controls (13.7%; 7 of 51) was observed. Sequencing of nucleocapsid protein (N) gene amplicons revealed point mutations in OC43, some consistently found in three MS patient brains and one normal control but never observed in laboratory viruses. In situ hybridization confirmed the presence of viral RNA in brain parenchyma, outside blood vessels. The presence of HCoV in human brains is consistent with neuroinvasion by these respiratory pathogens. Further studies are needed to distinguish between opportunistic and disease-associated viral presence in human brains.

Authors+Show Affiliations

Laboratory of Neuroimmunovirology, Human Health Research Center, INRS-Armand-Frappier Institute, University of Quebec, Laval, Québec, Canada H7V 1B7.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10982334

Citation

Arbour, N, et al. "Neuroinvasion By Human Respiratory Coronaviruses." Journal of Virology, vol. 74, no. 19, 2000, pp. 8913-21.
Arbour N, Day R, Newcombe J, et al. Neuroinvasion by human respiratory coronaviruses. J Virol. 2000;74(19):8913-21.
Arbour, N., Day, R., Newcombe, J., & Talbot, P. J. (2000). Neuroinvasion by human respiratory coronaviruses. Journal of Virology, 74(19), 8913-21.
Arbour N, et al. Neuroinvasion By Human Respiratory Coronaviruses. J Virol. 2000;74(19):8913-21. PubMed PMID: 10982334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroinvasion by human respiratory coronaviruses. AU - Arbour,N, AU - Day,R, AU - Newcombe,J, AU - Talbot,P J, PY - 2000/9/12/pubmed PY - 2000/10/7/medline PY - 2000/9/12/entrez SP - 8913 EP - 21 JF - Journal of virology JO - J Virol VL - 74 IS - 19 N2 - Human coronaviruses (HCoV) cause common colds but can also infect neural cell cultures. To provide definitive experimental evidence for the neurotropism and neuroinvasion of HCoV and its possible association with multiple sclerosis (MS), we have performed an extensive search and characterization of HCoV RNA in a large panel of human brain autopsy samples. Very stringent reverse transcription-PCR with two primer pairs for both viral strains (229E and OC43), combined with Southern hybridization, was performed on samples from 90 coded donors with various neurological diseases (39 with MS and 26 with other neurological diseases) or normal controls (25 patients). We report that 44% (40 of 90) of donors were positive for 229E and that 23% (21 of 90) were positive for OC43. A statistically significant higher prevalence of OC43 in MS patients (35.9%; 14 of 39) than in controls (13.7%; 7 of 51) was observed. Sequencing of nucleocapsid protein (N) gene amplicons revealed point mutations in OC43, some consistently found in three MS patient brains and one normal control but never observed in laboratory viruses. In situ hybridization confirmed the presence of viral RNA in brain parenchyma, outside blood vessels. The presence of HCoV in human brains is consistent with neuroinvasion by these respiratory pathogens. Further studies are needed to distinguish between opportunistic and disease-associated viral presence in human brains. SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/10982334/full_citation L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=10982334 DB - PRIME DP - Unbound Medicine ER -