Tags

Type your tag names separated by a space and hit enter

Demyelination determinants map to the spike glycoprotein gene of coronavirus mouse hepatitis virus.
J Virol. 2000 Oct; 74(19):9206-13.JV

Abstract

Demyelination is the pathologic hallmark of the human immune-mediated neurologic disease multiple sclerosis, which may be triggered or exacerbated by viral infections. Several experimental animal models have been developed to study the mechanism of virus-induced demyelination, including coronavirus mouse hepatitis virus (MHV) infection in mice. The envelope spike (S) glycoprotein of MHV contains determinants of properties essential for virus-host interactions. However, the molecular determinants of MHV-induced demyelination are still unknown. To investigate the mechanism of MHV-induced demyelination, we examined whether the S gene of MHV contains determinants of demyelination and whether demyelination is linked to viral persistence. Using targeted RNA recombination, we replaced the S gene of a demyelinating virus (MHV-A59) with the S gene of a closely related, nondemyelinating virus (MHV-2). Recombinant viruses containing an S gene derived from MHV-2 in an MHV-A59 background (Penn98-1 and Penn98-2) exhibited a persistence-positive, demyelination-negative phenotype. Thus, determinants of demyelination map to the S gene of MHV. Furthermore, viral persistence is insufficient to induce demyelination, although it may be a prerequisite for the development of demyelination.

Authors+Show Affiliations

Division of Neuropathology, Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10982367

Citation

Das Sarma, J, et al. "Demyelination Determinants Map to the Spike Glycoprotein Gene of Coronavirus Mouse Hepatitis Virus." Journal of Virology, vol. 74, no. 19, 2000, pp. 9206-13.
Das Sarma J, Fu L, Tsai JC, et al. Demyelination determinants map to the spike glycoprotein gene of coronavirus mouse hepatitis virus. J Virol. 2000;74(19):9206-13.
Das Sarma, J., Fu, L., Tsai, J. C., Weiss, S. R., & Lavi, E. (2000). Demyelination determinants map to the spike glycoprotein gene of coronavirus mouse hepatitis virus. Journal of Virology, 74(19), 9206-13.
Das Sarma J, et al. Demyelination Determinants Map to the Spike Glycoprotein Gene of Coronavirus Mouse Hepatitis Virus. J Virol. 2000;74(19):9206-13. PubMed PMID: 10982367.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Demyelination determinants map to the spike glycoprotein gene of coronavirus mouse hepatitis virus. AU - Das Sarma,J, AU - Fu,L, AU - Tsai,J C, AU - Weiss,S R, AU - Lavi,E, PY - 2000/9/12/pubmed PY - 2000/10/7/medline PY - 2000/9/12/entrez SP - 9206 EP - 13 JF - Journal of virology JO - J Virol VL - 74 IS - 19 N2 - Demyelination is the pathologic hallmark of the human immune-mediated neurologic disease multiple sclerosis, which may be triggered or exacerbated by viral infections. Several experimental animal models have been developed to study the mechanism of virus-induced demyelination, including coronavirus mouse hepatitis virus (MHV) infection in mice. The envelope spike (S) glycoprotein of MHV contains determinants of properties essential for virus-host interactions. However, the molecular determinants of MHV-induced demyelination are still unknown. To investigate the mechanism of MHV-induced demyelination, we examined whether the S gene of MHV contains determinants of demyelination and whether demyelination is linked to viral persistence. Using targeted RNA recombination, we replaced the S gene of a demyelinating virus (MHV-A59) with the S gene of a closely related, nondemyelinating virus (MHV-2). Recombinant viruses containing an S gene derived from MHV-2 in an MHV-A59 background (Penn98-1 and Penn98-2) exhibited a persistence-positive, demyelination-negative phenotype. Thus, determinants of demyelination map to the S gene of MHV. Furthermore, viral persistence is insufficient to induce demyelination, although it may be a prerequisite for the development of demyelination. SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/10982367/Demyelination_determinants_map_to_the_spike_glycoprotein_gene_of_coronavirus_mouse_hepatitis_virus_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=10982367 DB - PRIME DP - Unbound Medicine ER -