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Characterization of grape procyanidins using high-performance liquid Chromatography/Mass spectrometry and matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry.
J Agric Food Chem. 2000 Sep; 48(9):3990-6.JA

Abstract

High-performance liquid chromatography/mass spectrometry (HPLC/MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to characterize the procyanidin composition of the grape seed extract. The detection of the oligomers composed of (+)-catechin, (-)-epicatechin, and their galloylated derivatives in the grape seeds is demonstrated. With MALDI-TOF MS, oligomers up to nonamers were observed. The potential of the MALDI-TOF MS technique as a quantification tool is also discussed. The information presented in this study could lead to the determination of procyanidin content and their molecular weight distribution in grape seeds.

Authors+Show Affiliations

Science and Technology, Givaudan Flavors Corporation, 1199 Edison Drive, Cincinnati, Ohio 45216, USA. ying.yang@givaudan.comNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10995302

Citation

Yang, Y, and M Chien. "Characterization of Grape Procyanidins Using High-performance Liquid Chromatography/Mass Spectrometry and Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry." Journal of Agricultural and Food Chemistry, vol. 48, no. 9, 2000, pp. 3990-6.
Yang Y, Chien M. Characterization of grape procyanidins using high-performance liquid Chromatography/Mass spectrometry and matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry. J Agric Food Chem. 2000;48(9):3990-6.
Yang, Y., & Chien, M. (2000). Characterization of grape procyanidins using high-performance liquid Chromatography/Mass spectrometry and matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry. Journal of Agricultural and Food Chemistry, 48(9), 3990-6.
Yang Y, Chien M. Characterization of Grape Procyanidins Using High-performance Liquid Chromatography/Mass Spectrometry and Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry. J Agric Food Chem. 2000;48(9):3990-6. PubMed PMID: 10995302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of grape procyanidins using high-performance liquid Chromatography/Mass spectrometry and matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry. AU - Yang,Y, AU - Chien,M, PY - 2000/9/20/pubmed PY - 2001/2/28/medline PY - 2000/9/20/entrez SP - 3990 EP - 6 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 48 IS - 9 N2 - High-performance liquid chromatography/mass spectrometry (HPLC/MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to characterize the procyanidin composition of the grape seed extract. The detection of the oligomers composed of (+)-catechin, (-)-epicatechin, and their galloylated derivatives in the grape seeds is demonstrated. With MALDI-TOF MS, oligomers up to nonamers were observed. The potential of the MALDI-TOF MS technique as a quantification tool is also discussed. The information presented in this study could lead to the determination of procyanidin content and their molecular weight distribution in grape seeds. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/10995302/Characterization_of_grape_procyanidins_using_high_performance_liquid_Chromatography/Mass_spectrometry_and_matrix_assisted_laser_Desorption/Ionization_time_of_flight_mass_spectrometry_ L2 - https://doi.org/10.1021/jf000316q DB - PRIME DP - Unbound Medicine ER -