TY - JOUR T1 - Cloning and localization of the hyperpolarization-activated cyclic nucleotide-gated channel family in rat brain. AU - Monteggia,L M, AU - Eisch,A J, AU - Tang,M D, AU - Kaczmarek,L K, AU - Nestler,E J, PY - 2000/9/23/pubmed PY - 2001/2/28/medline PY - 2000/9/23/entrez SP - 129 EP - 39 JF - Brain research. Molecular brain research JO - Brain Res Mol Brain Res VL - 81 IS - 1-2 N2 - Rhythmic firing in brain and heart is mediated by pacemaker channels that are activated by hyperpolarization and regulated directly by cyclic nucleotides. Recent work has identified a new gene family that encodes such channels, which are termed hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels. In this study, we report the molecular cloning and localization by in situ hybridization of HCN1-4 in adult rat brain. The rat HCN1-4 clones show high homology to the deduced amino acid sequence of the mouse channels (>97% identity). The mRNA expression of the four channels in adult brain was evaluated in a systematic manner from the olfactory bulb to lower brain stem nuclei. Each mRNA demonstrated a unique pattern of distribution. HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus; HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body; HCN3 expression is most pronounced in supraoptic nucleus of hypothalamus; and HCN4 expression is most abundant in medial habenula and anterior and principal relay nuclei of the thalamus. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems. SN - 0169-328X UR - https://www.unboundmedicine.com/medline/citation/11000485/Cloning_and_localization_of_the_hyperpolarization_activated_cyclic_nucleotide_gated_channel_family_in_rat_brain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0169328X00001558 DB - PRIME DP - Unbound Medicine ER -