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Quantitative analysis of pharmaceutically active peptides using on-capillary analyte preconcentration transient isotachophoresis.
Electrophoresis. 2000 Aug; 21(14):2851-8.E

Abstract

An on-capillary adsorptive phase in combination with capillary electrophoresis (CE), frequently referred to as preconcentration CE, for quantitative analysis of low peptide concentrations was developed. The capillary containing the on-line analyte preconcentrator can be constructed within 5 min from commercially available extraction disks. These disks contain poly(styrenedivinylbenzene) adsorbent particles incorporated in a matrix of inert Teflon, creating a mechanically stable sorbent. Therefore, no frits are needed in the capillary to hold the stationary phase in place. Several parameters, such as the required minimal elution volume, required elution strength, sample application speed or ionic strength, and the capacity were investigated and special interest was given to the quantitative properties of the method. Instead of nL injections, volumes up to a least 25 microL are possible, yielding improvements in detection limits of 3-4 orders of magnitude. The observed limit of detection for both model peptides was 20 pg, corresponding to a 20 microL injection of a 1 ng/mL solution of both model peptides. Using low-wavelength UV detection, reproducibility and linearity in the low nanogram range were satisfactory. No influence of matrix salt concentrations was observed, extending the use of CE to all kinds of samples.

Authors+Show Affiliations

Universteit Utrecht, Faculty of Pharmacy, Department of Pharmaceutical Analysis, The Netherlands. j.c.m.waterval@pharm.uu.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11001294

Citation

Waterval, J C., et al. "Quantitative Analysis of Pharmaceutically Active Peptides Using On-capillary Analyte Preconcentration Transient Isotachophoresis." Electrophoresis, vol. 21, no. 14, 2000, pp. 2851-8.
Waterval JC, Hommels G, Teeuwsen J, et al. Quantitative analysis of pharmaceutically active peptides using on-capillary analyte preconcentration transient isotachophoresis. Electrophoresis. 2000;21(14):2851-8.
Waterval, J. C., Hommels, G., Teeuwsen, J., Bult, A., Lingeman, H., & Underberg, W. J. (2000). Quantitative analysis of pharmaceutically active peptides using on-capillary analyte preconcentration transient isotachophoresis. Electrophoresis, 21(14), 2851-8.
Waterval JC, et al. Quantitative Analysis of Pharmaceutically Active Peptides Using On-capillary Analyte Preconcentration Transient Isotachophoresis. Electrophoresis. 2000;21(14):2851-8. PubMed PMID: 11001294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative analysis of pharmaceutically active peptides using on-capillary analyte preconcentration transient isotachophoresis. AU - Waterval,J C, AU - Hommels,G, AU - Teeuwsen,J, AU - Bult,A, AU - Lingeman,H, AU - Underberg,W J, PY - 2000/9/23/pubmed PY - 2001/3/3/medline PY - 2000/9/23/entrez SP - 2851 EP - 8 JF - Electrophoresis JO - Electrophoresis VL - 21 IS - 14 N2 - An on-capillary adsorptive phase in combination with capillary electrophoresis (CE), frequently referred to as preconcentration CE, for quantitative analysis of low peptide concentrations was developed. The capillary containing the on-line analyte preconcentrator can be constructed within 5 min from commercially available extraction disks. These disks contain poly(styrenedivinylbenzene) adsorbent particles incorporated in a matrix of inert Teflon, creating a mechanically stable sorbent. Therefore, no frits are needed in the capillary to hold the stationary phase in place. Several parameters, such as the required minimal elution volume, required elution strength, sample application speed or ionic strength, and the capacity were investigated and special interest was given to the quantitative properties of the method. Instead of nL injections, volumes up to a least 25 microL are possible, yielding improvements in detection limits of 3-4 orders of magnitude. The observed limit of detection for both model peptides was 20 pg, corresponding to a 20 microL injection of a 1 ng/mL solution of both model peptides. Using low-wavelength UV detection, reproducibility and linearity in the low nanogram range were satisfactory. No influence of matrix salt concentrations was observed, extending the use of CE to all kinds of samples. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11001294/Quantitative_analysis_of_pharmaceutically_active_peptides_using_on_capillary_analyte_preconcentration_transient_isotachophoresis_ L2 - https://doi.org/10.1002/1522-2683(20000801)21:14<2851::AID-ELPS2851>3.0.CO;2-Q DB - PRIME DP - Unbound Medicine ER -
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