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Nitric oxide prevents tumor necrosis factor alpha-induced rat hepatocyte apoptosis by the interruption of mitochondrial apoptotic signaling through S-nitrosylation of caspase-8.

Abstract

Mitochondrial cytochrome c release plays a critical role in apoptotic signal cascade after the activation of cell surface death receptors. We investigated the role played by nitric oxide (NO) in mitochondrial apoptotic signaling in tumor necrosis factor alpha (TNF-alpha) plus actinomycin D (TNF-alpha/ActD)-induced apoptosis. NO produced either by S-nitroso-N-acetyl-DL-penicillamine (SNAP) or inducible NO synthase (iNOS) prevented TNF-alpha/ActD-induced apoptosis in hepatocytes and also inhibited both caspase-8-like (IETDase) and caspase-3-like protease (DEVDase) activity as well as mitochondrial cytochrome c release. Recombinant human (rh) caspase-8 induced the cleavage of the cytochrome c-effluxing factor Bid and cytochrome c release from purified mitochondria in the reconstitution system with Bid(+/+) cytosol, but not with Bid(-/-) cytosol. The addition of SNAP and the caspase-8 inhibitor Ac-IETD-fmk inhibited caspase-8-dependent Bid cleavage and cytochrome c release. The inhibitory effect of NO on caspase-8 was reversed by dithiothreitol (DTT). Furthermore, rh-caspase-8 was found to be modified by S-nitrosylation with 1.7 moles of NO bound per mole of enzyme. Treatment of hepatocytes with interleukin 1beta (IL-1beta) plus interferon gamma (IFN-gamma), which induced iNOS expression and NO production, suppressed TNF-alpha/ActD-induced Bid cleavage and mitochondrial cytochrome c release. The NOS inhibitor N(G)-monomethyl-L-arginine (NMA) inhibited the protective effects of IL-1beta and IFN-gamma. The liver-specific NO donor V-PYRRO/NO also inhibited in vivo elevation of IETDase activity, Bid cleavage, and mitochondrial cytochrome c release in the livers of rats injected with TNF-alpha plus D-galactosamine. Our results indicate that one mechanism by which NO protects hepatocytes from TNF-alpha/ActD-induced apoptosis is via the interruption of mitochondrial apoptotic signaling through S-nitrosylation of caspase-8.

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  • Authors+Show Affiliations

    ,

    Department of Molecular and Cellular Biochemistry, College of Medicine, Kangwon National University, Chunchon, Kangwon-do, Korea. ymkim@cc.kangwon.ac.kr

    , , , ,

    Source

    Hepatology (Baltimore, Md.) 32:4 Pt 1 2000 Oct pg 770-8

    MeSH

    Animals
    Apoptosis
    BH3 Interacting Domain Death Agonist Protein
    Carrier Proteins
    Caspase 3
    Caspase 8
    Caspase 9
    Caspase Inhibitors
    Caspases
    Cells, Cultured
    Cytochrome c Group
    Dactinomycin
    Male
    Mitochondria, Liver
    Nitric Oxide
    Penicillamine
    Rats
    Rats, Sprague-Dawley
    Tumor Necrosis Factor-alpha

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't
    Research Support, U.S. Gov't, P.H.S.

    Language

    eng

    PubMed ID

    11003621

    Citation

    Kim, Y M., et al. "Nitric Oxide Prevents Tumor Necrosis Factor Alpha-induced Rat Hepatocyte Apoptosis By the Interruption of Mitochondrial Apoptotic Signaling Through S-nitrosylation of Caspase-8." Hepatology (Baltimore, Md.), vol. 32, no. 4 Pt 1, 2000, pp. 770-8.
    Kim YM, Kim TH, Chung HT, et al. Nitric oxide prevents tumor necrosis factor alpha-induced rat hepatocyte apoptosis by the interruption of mitochondrial apoptotic signaling through S-nitrosylation of caspase-8. Hepatology. 2000;32(4 Pt 1):770-8.
    Kim, Y. M., Kim, T. H., Chung, H. T., Talanian, R. V., Yin, X. M., & Billiar, T. R. (2000). Nitric oxide prevents tumor necrosis factor alpha-induced rat hepatocyte apoptosis by the interruption of mitochondrial apoptotic signaling through S-nitrosylation of caspase-8. Hepatology (Baltimore, Md.), 32(4 Pt 1), pp. 770-8.
    Kim YM, et al. Nitric Oxide Prevents Tumor Necrosis Factor Alpha-induced Rat Hepatocyte Apoptosis By the Interruption of Mitochondrial Apoptotic Signaling Through S-nitrosylation of Caspase-8. Hepatology. 2000;32(4 Pt 1):770-8. PubMed PMID: 11003621.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Nitric oxide prevents tumor necrosis factor alpha-induced rat hepatocyte apoptosis by the interruption of mitochondrial apoptotic signaling through S-nitrosylation of caspase-8. AU - Kim,Y M, AU - Kim,T H, AU - Chung,H T, AU - Talanian,R V, AU - Yin,X M, AU - Billiar,T R, PY - 2000/9/26/pubmed PY - 2000/10/21/medline PY - 2000/9/26/entrez SP - 770 EP - 8 JF - Hepatology (Baltimore, Md.) JO - Hepatology VL - 32 IS - 4 Pt 1 N2 - Mitochondrial cytochrome c release plays a critical role in apoptotic signal cascade after the activation of cell surface death receptors. We investigated the role played by nitric oxide (NO) in mitochondrial apoptotic signaling in tumor necrosis factor alpha (TNF-alpha) plus actinomycin D (TNF-alpha/ActD)-induced apoptosis. NO produced either by S-nitroso-N-acetyl-DL-penicillamine (SNAP) or inducible NO synthase (iNOS) prevented TNF-alpha/ActD-induced apoptosis in hepatocytes and also inhibited both caspase-8-like (IETDase) and caspase-3-like protease (DEVDase) activity as well as mitochondrial cytochrome c release. Recombinant human (rh) caspase-8 induced the cleavage of the cytochrome c-effluxing factor Bid and cytochrome c release from purified mitochondria in the reconstitution system with Bid(+/+) cytosol, but not with Bid(-/-) cytosol. The addition of SNAP and the caspase-8 inhibitor Ac-IETD-fmk inhibited caspase-8-dependent Bid cleavage and cytochrome c release. The inhibitory effect of NO on caspase-8 was reversed by dithiothreitol (DTT). Furthermore, rh-caspase-8 was found to be modified by S-nitrosylation with 1.7 moles of NO bound per mole of enzyme. Treatment of hepatocytes with interleukin 1beta (IL-1beta) plus interferon gamma (IFN-gamma), which induced iNOS expression and NO production, suppressed TNF-alpha/ActD-induced Bid cleavage and mitochondrial cytochrome c release. The NOS inhibitor N(G)-monomethyl-L-arginine (NMA) inhibited the protective effects of IL-1beta and IFN-gamma. The liver-specific NO donor V-PYRRO/NO also inhibited in vivo elevation of IETDase activity, Bid cleavage, and mitochondrial cytochrome c release in the livers of rats injected with TNF-alpha plus D-galactosamine. Our results indicate that one mechanism by which NO protects hepatocytes from TNF-alpha/ActD-induced apoptosis is via the interruption of mitochondrial apoptotic signaling through S-nitrosylation of caspase-8. SN - 0270-9139 UR - https://www.unboundmedicine.com/medline/citation/11003621/Nitric_oxide_prevents_tumor_necrosis_factor_alpha_induced_rat_hepatocyte_apoptosis_by_the_interruption_of_mitochondrial_apoptotic_signaling_through_S_nitrosylation_of_caspase_8_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0270913900896731 DB - PRIME DP - Unbound Medicine ER -