Tags

Type your tag names separated by a space and hit enter

Amelioration of microvascular myocardial ischemia by gene transfer of vascular endothelial growth factor in rabbits.
J Thorac Cardiovasc Surg. 2000 Oct; 120(4):720-8.JT

Abstract

OBJECTIVES

Restoration of coronary blood flow by angiogenesis may offer a new approach to intractable ischemic heart disease. In the present study, we investigated the angiogenic effects of gene transfer of vascular endothelial growth factor 165 on microvascular myocardial ischemia.

METHODS

A rabbit model of microvascular myocardial ischemia was created by plugging coronary microvessels with microspheres (15 microm in diameter, 2.8 x 10(5)/kg, n = 29). Gene transfer was performed by semi-selective intracoronary injection of recombinant adenovirus expressing vascular endothelial growth factor 165 forty minutes after microsphere injection (n = 9).

RESULTS

Microsphere injection reduced myocardial perfusion (78% +/- 9% of baseline tissue flow) and diminished myocardial contraction (61% +/- 12% of the baseline ejection fraction) and cardiac performance (elevated left ventricular end-diastolic pressure and decreased systemic flow) in the acute phase. At 17 +/- 3 days, gene transfer of vascular endothelial growth factor 165 had had the following effects: (1) promoted coronary angiogenesis as evidenced by myocardial flow above the baseline (121% +/- 24%), (2) increased vascular density revealed by synchrotron radiation microangiography and histologic analysis, (3) ameliorated the degree of myocardial ischemia as evidenced by myocardial lactate content and the extent of histologic necrosis, and (4) restored heart function as evidenced by increased ejection fraction (95% +/- 10%), reduced left ventricular end-diastolic pressure, and restored body weight.

CONCLUSIONS

In vivo vascular endothelial growth factor 165 gene transfer promoted angiogenesis and was an effective approach to treating microvascular myocardial ischemia.

Authors+Show Affiliations

Department of Physiology, the Reserch Center for Genetic Engineering and Cell Transplantation, Tokai University School of Medicine, Isehara, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11003755

Citation

Tanaka, E, et al. "Amelioration of Microvascular Myocardial Ischemia By Gene Transfer of Vascular Endothelial Growth Factor in Rabbits." The Journal of Thoracic and Cardiovascular Surgery, vol. 120, no. 4, 2000, pp. 720-8.
Tanaka E, Hattan N, Ando K, et al. Amelioration of microvascular myocardial ischemia by gene transfer of vascular endothelial growth factor in rabbits. J Thorac Cardiovasc Surg. 2000;120(4):720-8.
Tanaka, E., Hattan, N., Ando, K., Ueno, H., Sugio, Y., Mohammed, M. U., Voltchikhina, S. A., & Mori, H. (2000). Amelioration of microvascular myocardial ischemia by gene transfer of vascular endothelial growth factor in rabbits. The Journal of Thoracic and Cardiovascular Surgery, 120(4), 720-8.
Tanaka E, et al. Amelioration of Microvascular Myocardial Ischemia By Gene Transfer of Vascular Endothelial Growth Factor in Rabbits. J Thorac Cardiovasc Surg. 2000;120(4):720-8. PubMed PMID: 11003755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amelioration of microvascular myocardial ischemia by gene transfer of vascular endothelial growth factor in rabbits. AU - Tanaka,E, AU - Hattan,N, AU - Ando,K, AU - Ueno,H, AU - Sugio,Y, AU - Mohammed,M U, AU - Voltchikhina,S A, AU - Mori,H, PY - 2000/9/26/pubmed PY - 2001/2/28/medline PY - 2000/9/26/entrez SP - 720 EP - 8 JF - The Journal of thoracic and cardiovascular surgery JO - J. Thorac. Cardiovasc. Surg. VL - 120 IS - 4 N2 - OBJECTIVES: Restoration of coronary blood flow by angiogenesis may offer a new approach to intractable ischemic heart disease. In the present study, we investigated the angiogenic effects of gene transfer of vascular endothelial growth factor 165 on microvascular myocardial ischemia. METHODS: A rabbit model of microvascular myocardial ischemia was created by plugging coronary microvessels with microspheres (15 microm in diameter, 2.8 x 10(5)/kg, n = 29). Gene transfer was performed by semi-selective intracoronary injection of recombinant adenovirus expressing vascular endothelial growth factor 165 forty minutes after microsphere injection (n = 9). RESULTS: Microsphere injection reduced myocardial perfusion (78% +/- 9% of baseline tissue flow) and diminished myocardial contraction (61% +/- 12% of the baseline ejection fraction) and cardiac performance (elevated left ventricular end-diastolic pressure and decreased systemic flow) in the acute phase. At 17 +/- 3 days, gene transfer of vascular endothelial growth factor 165 had had the following effects: (1) promoted coronary angiogenesis as evidenced by myocardial flow above the baseline (121% +/- 24%), (2) increased vascular density revealed by synchrotron radiation microangiography and histologic analysis, (3) ameliorated the degree of myocardial ischemia as evidenced by myocardial lactate content and the extent of histologic necrosis, and (4) restored heart function as evidenced by increased ejection fraction (95% +/- 10%), reduced left ventricular end-diastolic pressure, and restored body weight. CONCLUSIONS: In vivo vascular endothelial growth factor 165 gene transfer promoted angiogenesis and was an effective approach to treating microvascular myocardial ischemia. SN - 0022-5223 UR - https://www.unboundmedicine.com/medline/citation/11003755/Amelioration_of_microvascular_myocardial_ischemia_by_gene_transfer_of_vascular_endothelial_growth_factor_in_rabbits_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-5223(00)30163-5 DB - PRIME DP - Unbound Medicine ER -