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[Cytoimmunologic changes in material obtained from bronchoalveolar lavage (BAL) in asymptomatic individuals chronically exposed to silica dust].
Pneumonol Alergol Pol. 2000; 68(3-4):109-19.PA

Abstract

The aim of this study was to determine and to evaluate silica induced lung cell reactivity--if any--in bronchoalveolar space, before clinical changes develop. Bronchoalveolar lavage (BAL) was carried out in 15 nonsmoking individuals with chronic professional silica exposure, free of lung signs and symptoms. Controls were healthy nonsmokers. Routine BAL cytology (HE, MGG) was completed by mast cell staining (toluidine blue). BAL lymphocyte subsets were phenotyped by direct two- and three-color immunofluorescence (applied DAKO A/S monoclonal antibodies: anti-CD3, CD4, CD8, CD11b, CD14, CD15, CD16 + 56, CD19, CD25, CD45, HLA-DR). Parallel staining was performed in peripheral blood. In individuals with chronic silica exposure we found: significant increase in alveolar macrophage (362 +/- 45 vs 160 +/- 33 x 10(3) cells/ml, p < 0.05), lymphocyte (61 +/- 9 vs 24 +/- 5 x 10(3) cells/ml, p < 0.05) and BAL total cell (415 +/- 76 vs 187 +/- 34 x 10(3) cells/ml, p < 0.05) numbers; significant increase in mast cell (0.4 +/- 0.1 vs 0.2 +/- 0.1, p < 0.05), NK cell (7.0 +/- 1.8 vs 3.6 +/- 1.0, p < 0.05) and Th early activated lymphocyte percent (CD4 + CD25+ calculated as percentage of CD4+ cells: 15.1 +/- 1.5 vs 7.8 +/- 1.6, p < 0.01). All results were presented as median +/- SEM. Bronchoalveolar space of people with chronic silica exposure usually shows pathological reaction (especially macrophagic alveolitis), although they are free of manifested pulmonary disease. Th early activated lymphocytes, NK cells and mast cells seem to play important role in the early interstitial lung tissue reaction to silica.

Authors+Show Affiliations

Katedra Patofizjologii CM UJ, Oddział w Krakowie.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Controlled Clinical Trial
English Abstract
Journal Article

Language

pol

PubMed ID

11004845

Citation

Kopiński, P, et al. "[Cytoimmunologic Changes in Material Obtained From Bronchoalveolar Lavage (BAL) in Asymptomatic Individuals Chronically Exposed to Silica Dust]." Pneumonologia I Alergologia Polska, vol. 68, no. 3-4, 2000, pp. 109-19.
Kopiński P, Czunko P, Soja J, et al. [Cytoimmunologic changes in material obtained from bronchoalveolar lavage (BAL) in asymptomatic individuals chronically exposed to silica dust]. Pneumonol Alergol Pol. 2000;68(3-4):109-19.
Kopiński, P., Czunko, P., Soja, J., Lackowska, B., Gil, K., Jedynak, U., Szczeklik, J., Sładek, K., & Chłap, Z. (2000). [Cytoimmunologic changes in material obtained from bronchoalveolar lavage (BAL) in asymptomatic individuals chronically exposed to silica dust]. Pneumonologia I Alergologia Polska, 68(3-4), 109-19.
Kopiński P, et al. [Cytoimmunologic Changes in Material Obtained From Bronchoalveolar Lavage (BAL) in Asymptomatic Individuals Chronically Exposed to Silica Dust]. Pneumonol Alergol Pol. 2000;68(3-4):109-19. PubMed PMID: 11004845.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Cytoimmunologic changes in material obtained from bronchoalveolar lavage (BAL) in asymptomatic individuals chronically exposed to silica dust]. AU - Kopiński,P, AU - Czunko,P, AU - Soja,J, AU - Lackowska,B, AU - Gil,K, AU - Jedynak,U, AU - Szczeklik,J, AU - Sładek,K, AU - Chłap,Z, PY - 2000/9/27/pubmed PY - 2001/2/28/medline PY - 2000/9/27/entrez SP - 109 EP - 19 JF - Pneumonologia i alergologia polska JO - Pneumonol Alergol Pol VL - 68 IS - 3-4 N2 - The aim of this study was to determine and to evaluate silica induced lung cell reactivity--if any--in bronchoalveolar space, before clinical changes develop. Bronchoalveolar lavage (BAL) was carried out in 15 nonsmoking individuals with chronic professional silica exposure, free of lung signs and symptoms. Controls were healthy nonsmokers. Routine BAL cytology (HE, MGG) was completed by mast cell staining (toluidine blue). BAL lymphocyte subsets were phenotyped by direct two- and three-color immunofluorescence (applied DAKO A/S monoclonal antibodies: anti-CD3, CD4, CD8, CD11b, CD14, CD15, CD16 + 56, CD19, CD25, CD45, HLA-DR). Parallel staining was performed in peripheral blood. In individuals with chronic silica exposure we found: significant increase in alveolar macrophage (362 +/- 45 vs 160 +/- 33 x 10(3) cells/ml, p < 0.05), lymphocyte (61 +/- 9 vs 24 +/- 5 x 10(3) cells/ml, p < 0.05) and BAL total cell (415 +/- 76 vs 187 +/- 34 x 10(3) cells/ml, p < 0.05) numbers; significant increase in mast cell (0.4 +/- 0.1 vs 0.2 +/- 0.1, p < 0.05), NK cell (7.0 +/- 1.8 vs 3.6 +/- 1.0, p < 0.05) and Th early activated lymphocyte percent (CD4 + CD25+ calculated as percentage of CD4+ cells: 15.1 +/- 1.5 vs 7.8 +/- 1.6, p < 0.01). All results were presented as median +/- SEM. Bronchoalveolar space of people with chronic silica exposure usually shows pathological reaction (especially macrophagic alveolitis), although they are free of manifested pulmonary disease. Th early activated lymphocytes, NK cells and mast cells seem to play important role in the early interstitial lung tissue reaction to silica. SN - 0867-7077 UR - https://www.unboundmedicine.com/medline/citation/11004845/[Cytoimmunologic_changes_in_material_obtained_from_bronchoalveolar_lavage__BAL__in_asymptomatic_individuals_chronically_exposed_to_silica_dust]_ L2 - https://medlineplus.gov/occupationalhealth.html DB - PRIME DP - Unbound Medicine ER -