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Nitric oxide synthase activity and expression in experimental diabetic neuropathy.
J Neuropathol Exp Neurol. 2000 Sep; 59(9):798-807.JN

Abstract

The changes of nitric oxide synthase (NOS) activity and expression in experimental diabetic neuropathy have not been examined. Increases in ganglia NOS might be similar to those that follow axotomy, whereas declines in endothelial NOS (eNOS) and immunological NOS (iNOS) might explain dysfunction of microvessels or macrophages. In this work, we studied NOS activity in lumbar dorsal root ganglia (DRG) of rats with both short- and long-term experimental streptozotocin-induced diabetes and correlated it with expression of each of the 3 NOS isoforms. NOS enzymatic activity in DRG increased after 12 months of diabetes. This increase, however, was not accompanied by an increase in neuronal NOS immunohistochemistry or mRNA. Immunohistochemical and RT-PCR studies did not identify changes of eNOS expression in 12-month sciatic nerves or DRG from diabetics. Two-month diabetic DRG had increased eNOS mRNA and there was novel eNOS labeling of capsular DRG and perineurial cells. iNOS mRNA levels were lower in diabetics at both time points in peripheral nerves but were unchanged in DRG. Diabetic ganglia showed an increase in NOS activity not explained by novel NOS isoform synthesis. The increases may compensate for NO "quenching" by endproducts of glycosylation. Declines in iNOS may indicate impaired macrophage function.

Authors+Show Affiliations

Neuroscience Research Group and the Department of Clinical Neurosciences, University of Calgary, Alberta, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11005260

Citation

Zochodne, D W., et al. "Nitric Oxide Synthase Activity and Expression in Experimental Diabetic Neuropathy." Journal of Neuropathology and Experimental Neurology, vol. 59, no. 9, 2000, pp. 798-807.
Zochodne DW, Verge VM, Cheng C, et al. Nitric oxide synthase activity and expression in experimental diabetic neuropathy. J Neuropathol Exp Neurol. 2000;59(9):798-807.
Zochodne, D. W., Verge, V. M., Cheng, C., Höke, A., Jolley, C., Thomsen, K., Rubin, I., & Lauritzen, M. (2000). Nitric oxide synthase activity and expression in experimental diabetic neuropathy. Journal of Neuropathology and Experimental Neurology, 59(9), 798-807.
Zochodne DW, et al. Nitric Oxide Synthase Activity and Expression in Experimental Diabetic Neuropathy. J Neuropathol Exp Neurol. 2000;59(9):798-807. PubMed PMID: 11005260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide synthase activity and expression in experimental diabetic neuropathy. AU - Zochodne,D W, AU - Verge,V M, AU - Cheng,C, AU - Höke,A, AU - Jolley,C, AU - Thomsen,K, AU - Rubin,I, AU - Lauritzen,M, PY - 2000/9/27/pubmed PY - 2000/10/14/medline PY - 2000/9/27/entrez SP - 798 EP - 807 JF - Journal of neuropathology and experimental neurology JO - J Neuropathol Exp Neurol VL - 59 IS - 9 N2 - The changes of nitric oxide synthase (NOS) activity and expression in experimental diabetic neuropathy have not been examined. Increases in ganglia NOS might be similar to those that follow axotomy, whereas declines in endothelial NOS (eNOS) and immunological NOS (iNOS) might explain dysfunction of microvessels or macrophages. In this work, we studied NOS activity in lumbar dorsal root ganglia (DRG) of rats with both short- and long-term experimental streptozotocin-induced diabetes and correlated it with expression of each of the 3 NOS isoforms. NOS enzymatic activity in DRG increased after 12 months of diabetes. This increase, however, was not accompanied by an increase in neuronal NOS immunohistochemistry or mRNA. Immunohistochemical and RT-PCR studies did not identify changes of eNOS expression in 12-month sciatic nerves or DRG from diabetics. Two-month diabetic DRG had increased eNOS mRNA and there was novel eNOS labeling of capsular DRG and perineurial cells. iNOS mRNA levels were lower in diabetics at both time points in peripheral nerves but were unchanged in DRG. Diabetic ganglia showed an increase in NOS activity not explained by novel NOS isoform synthesis. The increases may compensate for NO "quenching" by endproducts of glycosylation. Declines in iNOS may indicate impaired macrophage function. SN - 0022-3069 UR - https://www.unboundmedicine.com/medline/citation/11005260/Nitric_oxide_synthase_activity_and_expression_in_experimental_diabetic_neuropathy_ L2 - https://academic.oup.com/jnen/article-lookup/doi/10.1093/jnen/59.9.798 DB - PRIME DP - Unbound Medicine ER -