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The fatty acid composition of chylomicrons influences the rate of their lipolysis in vivo.
Nutr Metab Cardiovasc Dis. 2000 Jun; 10(3):121-5.NM

Abstract

BACKGROUND AND AIMS

Previous work in our laboratory has shown that chylomicron triacylglycerol is lipolysed in vitro by lipoprotein lipase more rapidly when the particles are enriched with n-6 polyunsaturated as compared to saturated, monounsaturated or n-3 polyunsaturated fatty acids. It is possible, however, that this does not reflect the situation in vivo, where the active enzyme is bound to the vascular endothelium. The aim of the present study is to investigate the effect of the fatty acid composition of chylomicrons on their lipolysis in the rat in vivo.

METHODS AND RESULTS

[3H]Oleate-labelled chylomicrons derived from palm, olive, corn or fish oil (enriched in saturated, monounsaturated, n-6 polyunsaturated and n-3 polyunsaturated fatty acids respectively) containing > 90% of the label in triacylglycerol were injected intravenously into functionally hepatectomised rats and blood samples were taken at time intervals up to 40 min. The radioactivity in serum triacylglycerol decreased significantly more rapidly when corn oil as compared to palm, olive or fish oil chylomicrons were used. Conversely, the radioactivity in serum free fatty acid derived from corn oil chylomicrons showed a faster increase than that derived from the other three types of particles.

CONCLUSIONS

These results indicate that chylomicrons enriched with n-6 polyunsaturated fatty acids are converted to chylomicron remnants in vivo more rapidly than those enriched with saturated, monounsaturated or n-3 polyunsaturated fatty acids. This provides a partial explanation for the differential rate of removal from the blood of cholesterol carried in chylomicrons of different fatty acid composition demonstrated in previous work from this laboratory.

Authors+Show Affiliations

Rahman MH
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University.
Avella MA
No affiliation info available
Botham KM
No affiliation info available

MeSH

AnimalsChylomicronsDietary FatsEndothelium, VascularFatty AcidsKineticsLipolysisLipoprotein LipaseMaleOleic AcidRatsRats, WistarTime FactorsTriglycerides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11006920

Citation

Rahman, M H., et al. "The Fatty Acid Composition of Chylomicrons Influences the Rate of Their Lipolysis in Vivo." Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, vol. 10, no. 3, 2000, pp. 121-5.
Rahman MH, Avella MA, Botham KM. The fatty acid composition of chylomicrons influences the rate of their lipolysis in vivo. Nutr Metab Cardiovasc Dis. 2000;10(3):121-5.
Rahman, M. H., Avella, M. A., & Botham, K. M. (2000). The fatty acid composition of chylomicrons influences the rate of their lipolysis in vivo. Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, 10(3), 121-5.
Rahman MH, Avella MA, Botham KM. The Fatty Acid Composition of Chylomicrons Influences the Rate of Their Lipolysis in Vivo. Nutr Metab Cardiovasc Dis. 2000;10(3):121-5. PubMed PMID: 11006920.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The fatty acid composition of chylomicrons influences the rate of their lipolysis in vivo. AU - Rahman,M H, AU - Avella,M A, AU - Botham,K M, PY - 2000/9/28/pubmed PY - 2001/3/7/medline PY - 2000/9/28/entrez SP - 121 EP - 5 JF - Nutrition, metabolism, and cardiovascular diseases : NMCD JO - Nutr Metab Cardiovasc Dis VL - 10 IS - 3 N2 - BACKGROUND AND AIMS: Previous work in our laboratory has shown that chylomicron triacylglycerol is lipolysed in vitro by lipoprotein lipase more rapidly when the particles are enriched with n-6 polyunsaturated as compared to saturated, monounsaturated or n-3 polyunsaturated fatty acids. It is possible, however, that this does not reflect the situation in vivo, where the active enzyme is bound to the vascular endothelium. The aim of the present study is to investigate the effect of the fatty acid composition of chylomicrons on their lipolysis in the rat in vivo. METHODS AND RESULTS: [3H]Oleate-labelled chylomicrons derived from palm, olive, corn or fish oil (enriched in saturated, monounsaturated, n-6 polyunsaturated and n-3 polyunsaturated fatty acids respectively) containing > 90% of the label in triacylglycerol were injected intravenously into functionally hepatectomised rats and blood samples were taken at time intervals up to 40 min. The radioactivity in serum triacylglycerol decreased significantly more rapidly when corn oil as compared to palm, olive or fish oil chylomicrons were used. Conversely, the radioactivity in serum free fatty acid derived from corn oil chylomicrons showed a faster increase than that derived from the other three types of particles. CONCLUSIONS: These results indicate that chylomicrons enriched with n-6 polyunsaturated fatty acids are converted to chylomicron remnants in vivo more rapidly than those enriched with saturated, monounsaturated or n-3 polyunsaturated fatty acids. This provides a partial explanation for the differential rate of removal from the blood of cholesterol carried in chylomicrons of different fatty acid composition demonstrated in previous work from this laboratory. SN - 0939-4753 UR - https://www.unboundmedicine.com/medline/citation/11006920/The_fatty_acid_composition_of_chylomicrons_influences_the_rate_of_their_lipolysis_in_vivo_ L2 - https://medlineplus.gov/dietaryfats.html DB - PRIME DP - Unbound Medicine ER -