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[Increased activity of metalloproteinases and their inhibitors in cerebrospinal fluid of patients with tropical spastic paraparesis].
Rev Med Chil. 2000 Jun; 128(6):585-92.RM

Abstract

BACKGROUND

Proteolytic modifications of neuronal surfaces and the surrounding extracellular matrix are very important in neuronal development and regeneration. Increased activity of matrix metalloproteinases (MMPs) and their tissue inhibitors, due to secretion by macrophages and lymphocytes, occur in inflammatory processes that disrupt the blood brain barrier. However, neurons and microglia can also secrete these enzymes.

AIM

To identify the type of MMP present in the cerebrospinal fluid (CSF) and changes in the expression of tissue inhibitors of metalloproteinases (TIMPs) in patients with HTLV-1 associated tropical spastic paraparesis.

PATIENTS AND METHODS

CSF samples from 12 patients with HTLV-1 associated tropical spastic paraparesis and 12 healthy controls were obtained by an atraumatic lumbar puncture. The presence of MMPs was measured by zymography and the relative amounts of TIMPs were measured by immunowestern blot.

RESULTS

In the CSF of both controls and patients, a similar gelatinolytic band corresponding to proMMP-2 (latent form) was observed. In 83.3% of patients with HTLV 1 associated tropical spastic paraparesis, the MMP-9 was also present. TIMP-1, TIMP-2 and TIMP-3 were elevated 2.24 +/- 0.72, 3.85 +/- 1.38 and 5.89 +/- 3.4 fold, respectively, in the CSF of patients as compared to controls.

CONCLUSIONS

Patients with HTLV-1 associated tropical spastic paraparesis have elevated activity of MMP-9 and levels of TIMPs in the CSF, when compared to healthy controls.

Authors+Show Affiliations

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

spa

PubMed ID

11016056

Citation

Valenzuela, M A., et al. "[Increased Activity of Metalloproteinases and Their Inhibitors in Cerebrospinal Fluid of Patients With Tropical Spastic Paraparesis]." Revista Medica De Chile, vol. 128, no. 6, 2000, pp. 585-92.
Valenzuela MA, Collados L, Kettlun AM, et al. [Increased activity of metalloproteinases and their inhibitors in cerebrospinal fluid of patients with tropical spastic paraparesis]. Rev Med Chil. 2000;128(6):585-92.
Valenzuela, M. A., Collados, L., Kettlun, A. M., González, F., & Cartier, L. (2000). [Increased activity of metalloproteinases and their inhibitors in cerebrospinal fluid of patients with tropical spastic paraparesis]. Revista Medica De Chile, 128(6), 585-92.
Valenzuela MA, et al. [Increased Activity of Metalloproteinases and Their Inhibitors in Cerebrospinal Fluid of Patients With Tropical Spastic Paraparesis]. Rev Med Chil. 2000;128(6):585-92. PubMed PMID: 11016056.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Increased activity of metalloproteinases and their inhibitors in cerebrospinal fluid of patients with tropical spastic paraparesis]. AU - Valenzuela,M A, AU - Collados,L, AU - Kettlun,A M, AU - González,F, AU - Cartier,L, PY - 2000/10/4/pubmed PY - 2000/10/14/medline PY - 2000/10/4/entrez SP - 585 EP - 92 JF - Revista medica de Chile JO - Rev Med Chil VL - 128 IS - 6 N2 - BACKGROUND: Proteolytic modifications of neuronal surfaces and the surrounding extracellular matrix are very important in neuronal development and regeneration. Increased activity of matrix metalloproteinases (MMPs) and their tissue inhibitors, due to secretion by macrophages and lymphocytes, occur in inflammatory processes that disrupt the blood brain barrier. However, neurons and microglia can also secrete these enzymes. AIM: To identify the type of MMP present in the cerebrospinal fluid (CSF) and changes in the expression of tissue inhibitors of metalloproteinases (TIMPs) in patients with HTLV-1 associated tropical spastic paraparesis. PATIENTS AND METHODS: CSF samples from 12 patients with HTLV-1 associated tropical spastic paraparesis and 12 healthy controls were obtained by an atraumatic lumbar puncture. The presence of MMPs was measured by zymography and the relative amounts of TIMPs were measured by immunowestern blot. RESULTS: In the CSF of both controls and patients, a similar gelatinolytic band corresponding to proMMP-2 (latent form) was observed. In 83.3% of patients with HTLV 1 associated tropical spastic paraparesis, the MMP-9 was also present. TIMP-1, TIMP-2 and TIMP-3 were elevated 2.24 +/- 0.72, 3.85 +/- 1.38 and 5.89 +/- 3.4 fold, respectively, in the CSF of patients as compared to controls. CONCLUSIONS: Patients with HTLV-1 associated tropical spastic paraparesis have elevated activity of MMP-9 and levels of TIMPs in the CSF, when compared to healthy controls. SN - 0034-9887 UR - https://www.unboundmedicine.com/medline/citation/11016056/[Increased_activity_of_metalloproteinases_and_their_inhibitors_in_cerebrospinal_fluid_of_patients_with_tropical_spastic_paraparesis]_ L2 - http://www.diseaseinfosearch.org/result/7244 DB - PRIME DP - Unbound Medicine ER -