Tags

Type your tag names separated by a space and hit enter

Human p32: a coactivator for Epstein-Barr virus nuclear antigen-1-mediated transcriptional activation and possible role in viral latent cycle DNA replication.
Virology. 2000 Sep 15; 275(1):145-57.V

Abstract

The Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA-1) is required for the maintenance of the viral chromosome in latently infected, proliferating cells and plays a role in latent cycle DNA replication. EBNA-1 also functions as a positive and negative regulator of EBV gene expression. We have investigated the interaction of EBNA-1 with p32, a host mitochondrial protein that associates with EBNA-1 in EBV-positive Burkitt's lymphoma cells. Using a chromatin immunoprecipitation assay, we found that a fraction of p32 localizes to the viral latent cycle origin of DNA replication oriP in vivo. p32 binds EBNA-1 independently of other proteins or DNA. EBNA-1 variants lacking one of two p32 binding elements did not interact stably with p32 in cultured cells and were defective for both transcriptional activation of a reporter gene linked to oriP FR and replication and/or maintenance of a plasmid bearing oriP. These results support a role for p32 in transcriptional activation by EBNA-1 and suggest that p32 plays a role in EBV latent cycle DNA replication.

Authors+Show Affiliations

Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, New York 11794, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11017796

Citation

Van Scoy, S, et al. "Human P32: a Coactivator for Epstein-Barr Virus Nuclear Antigen-1-mediated Transcriptional Activation and Possible Role in Viral Latent Cycle DNA Replication." Virology, vol. 275, no. 1, 2000, pp. 145-57.
Van Scoy S, Watakabe I, Krainer AR, et al. Human p32: a coactivator for Epstein-Barr virus nuclear antigen-1-mediated transcriptional activation and possible role in viral latent cycle DNA replication. Virology. 2000;275(1):145-57.
Van Scoy, S., Watakabe, I., Krainer, A. R., & Hearing, J. (2000). Human p32: a coactivator for Epstein-Barr virus nuclear antigen-1-mediated transcriptional activation and possible role in viral latent cycle DNA replication. Virology, 275(1), 145-57.
Van Scoy S, et al. Human P32: a Coactivator for Epstein-Barr Virus Nuclear Antigen-1-mediated Transcriptional Activation and Possible Role in Viral Latent Cycle DNA Replication. Virology. 2000 Sep 15;275(1):145-57. PubMed PMID: 11017796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human p32: a coactivator for Epstein-Barr virus nuclear antigen-1-mediated transcriptional activation and possible role in viral latent cycle DNA replication. AU - Van Scoy,S, AU - Watakabe,I, AU - Krainer,A R, AU - Hearing,J, PY - 2000/10/6/pubmed PY - 2001/2/28/medline PY - 2000/10/6/entrez SP - 145 EP - 57 JF - Virology JO - Virology VL - 275 IS - 1 N2 - The Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA-1) is required for the maintenance of the viral chromosome in latently infected, proliferating cells and plays a role in latent cycle DNA replication. EBNA-1 also functions as a positive and negative regulator of EBV gene expression. We have investigated the interaction of EBNA-1 with p32, a host mitochondrial protein that associates with EBNA-1 in EBV-positive Burkitt's lymphoma cells. Using a chromatin immunoprecipitation assay, we found that a fraction of p32 localizes to the viral latent cycle origin of DNA replication oriP in vivo. p32 binds EBNA-1 independently of other proteins or DNA. EBNA-1 variants lacking one of two p32 binding elements did not interact stably with p32 in cultured cells and were defective for both transcriptional activation of a reporter gene linked to oriP FR and replication and/or maintenance of a plasmid bearing oriP. These results support a role for p32 in transcriptional activation by EBNA-1 and suggest that p32 plays a role in EBV latent cycle DNA replication. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/11017796/Human_p32:_a_coactivator_for_Epstein_Barr_virus_nuclear_antigen_1_mediated_transcriptional_activation_and_possible_role_in_viral_latent_cycle_DNA_replication_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(00)90508-7 DB - PRIME DP - Unbound Medicine ER -