Influence of cellular density on determination of EC(50) in microalgal growth inhibition tests.
Ecotoxicol Environ Saf. 2000 Oct; 47(2):112-6.EE

Abstract

Growth inhibition tests for copper were carried out on four marine microalgal species: Chlorella autotrophyca, Nannochloris atomus (Chlorophyceae), Phaeodactylum tricornutum (Bacillariophyceae), and Isochrysis aff. galbana (Primnesiophyceae). The test initial cellular densities were reduced to 50 and 10% from the recommended initial cellular density in most of standardized assays. OECD test protocol (originally described for freshwater) was adapted for seawater. The EC(50) values were reduced when initial cellular density decreased. The green algae used in this study exhibited lower sensitivity than P. tricornutum and quite lower than I. aff. galbana. The latter species was found to be very sensitive to copper. The concept of cellular toxic quote (amount of toxic per cell) is defined in order to improve the results of toxicity tests.

Authors+Show Affiliations

Moreno-Garrido I

Instituto de Ciencias Marinas de Andalucía, Polígono Río San Pedro, s/n., Puerto Real, Cádiz, 11510, Spain.

Lubián LM

No affiliation info available

Soares AM

No affiliation info available

MeSH

CopperCulture MediaEukaryotaLethal Dose 50Seawater

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11023688

Citation

Moreno-Garrido, I, et al. "Influence of Cellular Density On Determination of EC(50) in Microalgal Growth Inhibition Tests." Ecotoxicology and Environmental Safety, vol. 47, no. 2, 2000, pp. 112-6.

Moreno-Garrido I, Lubián LM, Soares AM. Influence of cellular density on determination of EC(50) in microalgal growth inhibition tests. Ecotoxicol Environ Saf. 2000;47(2):112-6.

Moreno-Garrido, I., Lubián, L. M., & Soares, A. M. (2000). Influence of cellular density on determination of EC(50) in microalgal growth inhibition tests. Ecotoxicology and Environmental Safety, 47(2), 112-6.

Moreno-Garrido I, Lubián LM, Soares AM. Influence of Cellular Density On Determination of EC(50) in Microalgal Growth Inhibition Tests. Ecotoxicol Environ Saf. 2000;47(2):112-6. PubMed PMID: 11023688.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Influence of cellular density on determination of EC(50) in microalgal growth inhibition tests. AU - Moreno-Garrido,I, AU - Lubián,L M, AU - Soares,A M, PY - 2000/10/12/pubmed PY - 2001/2/28/medline PY - 2000/10/12/entrez SP - 112 EP - 6 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 47 IS - 2 N2 - Growth inhibition tests for copper were carried out on four marine microalgal species: Chlorella autotrophyca, Nannochloris atomus (Chlorophyceae), Phaeodactylum tricornutum (Bacillariophyceae), and Isochrysis aff. galbana (Primnesiophyceae). The test initial cellular densities were reduced to 50 and 10% from the recommended initial cellular density in most of standardized assays. OECD test protocol (originally described for freshwater) was adapted for seawater. The EC(50) values were reduced when initial cellular density decreased. The green algae used in this study exhibited lower sensitivity than P. tricornutum and quite lower than I. aff. galbana. The latter species was found to be very sensitive to copper. The concept of cellular toxic quote (amount of toxic per cell) is defined in order to improve the results of toxicity tests. SN - 0147-6513 UR - https://www.unboundmedicine.com/medline/citation/11023688/Influence_of_cellular_density_on_determination_of_EC_50__in_microalgal_growth_inhibition_tests_ DB - PRIME DP - Unbound Medicine ER -

Tags

Influence of cellular density on determination of EC(50) in microalgal growth inhibition tests.Ecotoxicol Environ Saf. 2000 Oct; 47(2):112-6.EE