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Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs.
J Mol Biol. 2000 Oct 27; 303(3):395-403.JM

Abstract

Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation.

Authors+Show Affiliations

Center for Biophysics and COmputational BIology, University of Illinois at Urbana-Champaign, 61801, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11031116

Citation

Su, S, et al. "Crystal Structures of the Chromosomal Proteins Sso7d/Sac7d Bound to DNA Containing T-G Mismatched Base-pairs." Journal of Molecular Biology, vol. 303, no. 3, 2000, pp. 395-403.
Su S, Gao YG, Robinson H, et al. Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs. J Mol Biol. 2000;303(3):395-403.
Su, S., Gao, Y. G., Robinson, H., Liaw, Y. C., Edmondson, S. P., Shriver, J. W., & Wang, A. H. (2000). Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs. Journal of Molecular Biology, 303(3), 395-403.
Su S, et al. Crystal Structures of the Chromosomal Proteins Sso7d/Sac7d Bound to DNA Containing T-G Mismatched Base-pairs. J Mol Biol. 2000 Oct 27;303(3):395-403. PubMed PMID: 11031116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs. AU - Su,S, AU - Gao,Y G, AU - Robinson,H, AU - Liaw,Y C, AU - Edmondson,S P, AU - Shriver,J W, AU - Wang,A H, PY - 2000/10/14/pubmed PY - 2001/2/28/medline PY - 2000/10/14/entrez SP - 395 EP - 403 JF - Journal of molecular biology JO - J Mol Biol VL - 303 IS - 3 N2 - Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/11031116/Crystal_structures_of_the_chromosomal_proteins_Sso7d/Sac7d_bound_to_DNA_containing_T_G_mismatched_base_pairs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(00)94112-3 DB - PRIME DP - Unbound Medicine ER -