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Patients with bullous pemphigoid and linear IgA disease show a dual IgA and IgG autoimmune response to BP180.
J Autoimmun. 2000 Nov; 15(3):293-300.JA

Abstract

Bullous pemphigoid (BP) and linear IgA disease (LAD) are autoimmune subepidermal blistering skin diseases associated with autoantibodies against the transmembrane hemidesmosomal protein BP180/type XVII collagen. It has been demonstrated previously that BP is characterized predominantly by IgG autoantibodies, while autoantibodies in LAD mainly belong to the IgA isotype. The aim of the present study was to investigate the hypothesis that there is a significant overlap in the autoantibody isotype profiles associated with these two diseases. Several new recombinant forms of BP180 were generated in the baculovirus expression system, including the full-length protein. IgG autoantibodies to BP 180 were detectable in 39 of 40 (98%) of BP sera; interestingly, 88% of BP sera also contained IgA anti-BP180 autoantibodies. Similarly, anti-BP180 reactivity in LAD sera (n=22) was also attributed to both an IgA (68%) and an IgG (76%) autoantibody response. IgA and IgG autoantibodies to the intracellular portion of BP180 were found in 14% and 28% of BP sera, respectively, and in 8% of LAD sera (same percentage for both isotypes). Our findings clearly demonstrate that both BP and LAD patients have a dual IgA and IgG autoimmune response to BP180 which is directed not only to the ectodomain, but also to the intracellular portion of this protein.

Authors+Show Affiliations

Institute for Immunology, Pathology and Molecular Biology (IPM), Hamburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11040070

Citation

Kromminga, A, et al. "Patients With Bullous Pemphigoid and Linear IgA Disease Show a Dual IgA and IgG Autoimmune Response to BP180." Journal of Autoimmunity, vol. 15, no. 3, 2000, pp. 293-300.
Kromminga A, Scheckenbach C, Georgi M, et al. Patients with bullous pemphigoid and linear IgA disease show a dual IgA and IgG autoimmune response to BP180. J Autoimmun. 2000;15(3):293-300.
Kromminga, A., Scheckenbach, C., Georgi, M., Hagel, C., Arndt, R., Christophers, E., Bröcker, E. B., & Zillikens, D. (2000). Patients with bullous pemphigoid and linear IgA disease show a dual IgA and IgG autoimmune response to BP180. Journal of Autoimmunity, 15(3), 293-300.
Kromminga A, et al. Patients With Bullous Pemphigoid and Linear IgA Disease Show a Dual IgA and IgG Autoimmune Response to BP180. J Autoimmun. 2000;15(3):293-300. PubMed PMID: 11040070.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Patients with bullous pemphigoid and linear IgA disease show a dual IgA and IgG autoimmune response to BP180. AU - Kromminga,A, AU - Scheckenbach,C, AU - Georgi,M, AU - Hagel,C, AU - Arndt,R, AU - Christophers,E, AU - Bröcker,E B, AU - Zillikens,D, PY - 2000/10/21/pubmed PY - 2001/3/7/medline PY - 2000/10/21/entrez SP - 293 EP - 300 JF - Journal of autoimmunity JO - J Autoimmun VL - 15 IS - 3 N2 - Bullous pemphigoid (BP) and linear IgA disease (LAD) are autoimmune subepidermal blistering skin diseases associated with autoantibodies against the transmembrane hemidesmosomal protein BP180/type XVII collagen. It has been demonstrated previously that BP is characterized predominantly by IgG autoantibodies, while autoantibodies in LAD mainly belong to the IgA isotype. The aim of the present study was to investigate the hypothesis that there is a significant overlap in the autoantibody isotype profiles associated with these two diseases. Several new recombinant forms of BP180 were generated in the baculovirus expression system, including the full-length protein. IgG autoantibodies to BP 180 were detectable in 39 of 40 (98%) of BP sera; interestingly, 88% of BP sera also contained IgA anti-BP180 autoantibodies. Similarly, anti-BP180 reactivity in LAD sera (n=22) was also attributed to both an IgA (68%) and an IgG (76%) autoantibody response. IgA and IgG autoantibodies to the intracellular portion of BP180 were found in 14% and 28% of BP sera, respectively, and in 8% of LAD sera (same percentage for both isotypes). Our findings clearly demonstrate that both BP and LAD patients have a dual IgA and IgG autoimmune response to BP180 which is directed not only to the ectodomain, but also to the intracellular portion of this protein. SN - 0896-8411 UR - https://www.unboundmedicine.com/medline/citation/11040070/Patients_with_bullous_pemphigoid_and_linear_IgA_disease_show_a_dual_IgA_and_IgG_autoimmune_response_to_BP180_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0896-8411(00)90437-2 DB - PRIME DP - Unbound Medicine ER -