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Chiral separation of amino acids in biological fluids by micellar electrokinetic chromatography with laser-induced fluorescence detection.
J Chromatogr B Biomed Sci Appl. 2000 Aug 18; 745(2):389-97.JC

Abstract

A method is presented for the chiral analysis of amino acids in biological fluids using micellar electrokinetic chromatography (MEKC) and laser-induced fluorescence (LIF). The amino acids are derivatized with the chiral reagent (+/-)-1-(9-anthryl)-2-propyl chloroformate (APOC) and separated using a mixed micellar separation system. No tedious pre-purification of samples is required. The excellent separation efficiency and good detection capabilities of the MEKC-LIF system are exemplified in the analysis of urine and cerebrospinal fluid. This is the first time MEKC has been reported for chiral analysis of amino acids in biological fluids. The amino acids D-alanine, D-glutamine, and D-aspartic acid have been observed in cerebrospinal fluid, and D-alanine and D-glutamic acid in urine. To the best of our knowledge no measurements of either D-alanine in cerebrospinal fluid or D-glutamic acid in urine have been presented in the literature before.

Authors+Show Affiliations

Thorsén G
Department of Analytical Chemistry, Stockholm University, Sweden. gunnar.thorsen@anchem.su.se
Bergquist J
No affiliation info available

MeSH

AdultAmino AcidsChromatography, Micellar Electrokinetic CapillaryHumansLasersMaleSensitivity and SpecificitySpectrometry, FluorescenceStereoisomerism

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11043757

Citation

Thorsén, G, and J Bergquist. "Chiral Separation of Amino Acids in Biological Fluids By Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 745, no. 2, 2000, pp. 389-97.
Thorsén G, Bergquist J. Chiral separation of amino acids in biological fluids by micellar electrokinetic chromatography with laser-induced fluorescence detection. J Chromatogr B Biomed Sci Appl. 2000;745(2):389-97.
Thorsén, G., & Bergquist, J. (2000). Chiral separation of amino acids in biological fluids by micellar electrokinetic chromatography with laser-induced fluorescence detection. Journal of Chromatography. B, Biomedical Sciences and Applications, 745(2), 389-97.
Thorsén G, Bergquist J. Chiral Separation of Amino Acids in Biological Fluids By Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection. J Chromatogr B Biomed Sci Appl. 2000 Aug 18;745(2):389-97. PubMed PMID: 11043757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chiral separation of amino acids in biological fluids by micellar electrokinetic chromatography with laser-induced fluorescence detection. AU - Thorsén,G, AU - Bergquist,J, PY - 2000/10/24/pubmed PY - 2001/2/28/medline PY - 2000/10/24/entrez SP - 389 EP - 97 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J Chromatogr B Biomed Sci Appl VL - 745 IS - 2 N2 - A method is presented for the chiral analysis of amino acids in biological fluids using micellar electrokinetic chromatography (MEKC) and laser-induced fluorescence (LIF). The amino acids are derivatized with the chiral reagent (+/-)-1-(9-anthryl)-2-propyl chloroformate (APOC) and separated using a mixed micellar separation system. No tedious pre-purification of samples is required. The excellent separation efficiency and good detection capabilities of the MEKC-LIF system are exemplified in the analysis of urine and cerebrospinal fluid. This is the first time MEKC has been reported for chiral analysis of amino acids in biological fluids. The amino acids D-alanine, D-glutamine, and D-aspartic acid have been observed in cerebrospinal fluid, and D-alanine and D-glutamic acid in urine. To the best of our knowledge no measurements of either D-alanine in cerebrospinal fluid or D-glutamic acid in urine have been presented in the literature before. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/11043757/Chiral_separation_of_amino_acids_in_biological_fluids_by_micellar_electrokinetic_chromatography_with_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -