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Characterization of the glycoconjugates of boar testis and epididymis.
J Reprod Fertil. 2000 Nov; 120(2):325-35.JR

Abstract

Lectin histochemistry was used to perform in situ characterization of the glycoconjugates present in boar testis and epididymis. Thirteen horseradish peroxidase- or digoxigenin-labelled lectins were used in samples obtained from healthy fertile boars. The acrosomes of the spermatids were stained intensely by lectins with affinity for galactose and N-acetyl-galactosamine residues, these being soybean, peanut and Ricinus communis agglutinins. Sertoli cells were stained selectively by Maackia ammurensis agglutinin. The lamina propria of seminiferous tubules showed the most intense staining with fucose-binding lectins. The Golgi area and the apical part of the principal cells of the epididymis were stained intensely with many lectins and their distribution was similar in the three zones of the epididymis. On the basis of lectin affinity, both testis and epididymis appear to have N- and O-linked glycoconjugates. Spermatozoa from different epididymal regions showed different expression of terminal galactose and N-acetyl-galactosamine. Sialic acid (specifically alpha2,3 neuraminic-5 acid) was probably incorporated into spermatozoa along the extratesticular ducts. These findings indicate that the development and maturation of boar spermatozoa are accompanied by changes in glycoconjugates. As some lectins stain cellular or extracellular compartments specifically, these lectins could be useful markers in histopathological evaluation of diseases of boar testis and epididymis.

Authors+Show Affiliations

Department of Histology, School of Medicine, University of Navarra, Pamplona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11058448

Citation

Calvo, A, et al. "Characterization of the Glycoconjugates of Boar Testis and Epididymis." Journal of Reproduction and Fertility, vol. 120, no. 2, 2000, pp. 325-35.
Calvo A, Pastor LM, Bonet S, et al. Characterization of the glycoconjugates of boar testis and epididymis. J Reprod Fertil. 2000;120(2):325-35.
Calvo, A., Pastor, L. M., Bonet, S., Pinart, E., & Ventura, M. (2000). Characterization of the glycoconjugates of boar testis and epididymis. Journal of Reproduction and Fertility, 120(2), 325-35.
Calvo A, et al. Characterization of the Glycoconjugates of Boar Testis and Epididymis. J Reprod Fertil. 2000;120(2):325-35. PubMed PMID: 11058448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the glycoconjugates of boar testis and epididymis. AU - Calvo,A, AU - Pastor,L M, AU - Bonet,S, AU - Pinart,E, AU - Ventura,M, PY - 2000/11/4/pubmed PY - 2001/2/28/medline PY - 2000/11/4/entrez SP - 325 EP - 35 JF - Journal of reproduction and fertility JO - J. Reprod. Fertil. VL - 120 IS - 2 N2 - Lectin histochemistry was used to perform in situ characterization of the glycoconjugates present in boar testis and epididymis. Thirteen horseradish peroxidase- or digoxigenin-labelled lectins were used in samples obtained from healthy fertile boars. The acrosomes of the spermatids were stained intensely by lectins with affinity for galactose and N-acetyl-galactosamine residues, these being soybean, peanut and Ricinus communis agglutinins. Sertoli cells were stained selectively by Maackia ammurensis agglutinin. The lamina propria of seminiferous tubules showed the most intense staining with fucose-binding lectins. The Golgi area and the apical part of the principal cells of the epididymis were stained intensely with many lectins and their distribution was similar in the three zones of the epididymis. On the basis of lectin affinity, both testis and epididymis appear to have N- and O-linked glycoconjugates. Spermatozoa from different epididymal regions showed different expression of terminal galactose and N-acetyl-galactosamine. Sialic acid (specifically alpha2,3 neuraminic-5 acid) was probably incorporated into spermatozoa along the extratesticular ducts. These findings indicate that the development and maturation of boar spermatozoa are accompanied by changes in glycoconjugates. As some lectins stain cellular or extracellular compartments specifically, these lectins could be useful markers in histopathological evaluation of diseases of boar testis and epididymis. SN - 0022-4251 UR - https://www.unboundmedicine.com/medline/citation/11058448/Characterization_of_the_glycoconjugates_of_boar_testis_and_epididymis_ DB - PRIME DP - Unbound Medicine ER -