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Role of glutathione in heat-shock-induced cell death of Saccharomyces cerevisiae.
Biochem J. 2000 Nov 15; 352 Pt 1:71-8.BJ

Abstract

Previously we reported that expression of GSH1 (gamma-glutamylcysteine synthetase) and GSH2 (glutathione synthetase) of the yeast Saccharomyces cerevisiae was increased by heat-shock stress in a Yap1p-dependent fashion and consequently intracellular glutathione content was increased [Sugiyama, Izawa and Inoue (2000) J. Biol. Chem. 275, 15535-15540]. In the present study, we discuss the physiological role of glutathione in the heat-shock stress response in this yeast. Both gsh1 and gsh2 mutants could acquire thermotolerance by mild heat-shock stress and induction of Hsp104p in both mutants was normal; however, mutant cells died faster by heat shock than their parental wild-type strain. After pretreatment at a sublethal temperature, the number of respiration-deficient mutants increased in a gsh1 mutant strain in the early stages of exposure to a lethal temperature, although this increase was partially suppressed by the addition of glutathione. These results lead us to suspect that an increase of glutathione synthesis during heat-shock stress is to protect mitochondrial DNA from oxidative damage. To investigate the correlation between mitochondrial DNA damage and glutathione, mitochondrial Mn-superoxide dismutase (the SOD2 gene product) was disrupted. As a result, the rate of generation of respiration-deficient mutants of a sod2 delta strain was higher than that of the isogenic wild-type strain and treatment of the sod2 delta mutant with buthionine sulphoximine, an inhibitor of glutathione synthesis, inhibited cell growth. These results suggest that glutathione synthesis is induced by heat shock to protect the mitochondrial DNA from oxidative damage that may lead to cell death.

Authors+Show Affiliations

Research Institute for Food Science, Kyoto University, Uji, Kyoto 611-0011, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11062059

Citation

Sugiyama, K, et al. "Role of Glutathione in Heat-shock-induced Cell Death of Saccharomyces Cerevisiae." The Biochemical Journal, vol. 352 Pt 1, 2000, pp. 71-8.
Sugiyama K, Kawamura A, Izawa S, et al. Role of glutathione in heat-shock-induced cell death of Saccharomyces cerevisiae. Biochem J. 2000;352 Pt 1:71-8.
Sugiyama, K., Kawamura, A., Izawa, S., & Inoue, Y. (2000). Role of glutathione in heat-shock-induced cell death of Saccharomyces cerevisiae. The Biochemical Journal, 352 Pt 1, 71-8.
Sugiyama K, et al. Role of Glutathione in Heat-shock-induced Cell Death of Saccharomyces Cerevisiae. Biochem J. 2000 Nov 15;352 Pt 1:71-8. PubMed PMID: 11062059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of glutathione in heat-shock-induced cell death of Saccharomyces cerevisiae. AU - Sugiyama,K, AU - Kawamura,A, AU - Izawa,S, AU - Inoue,Y, PY - 2000/11/4/pubmed PY - 2001/2/28/medline PY - 2000/11/4/entrez SP - 71 EP - 8 JF - The Biochemical journal JO - Biochem J VL - 352 Pt 1 N2 - Previously we reported that expression of GSH1 (gamma-glutamylcysteine synthetase) and GSH2 (glutathione synthetase) of the yeast Saccharomyces cerevisiae was increased by heat-shock stress in a Yap1p-dependent fashion and consequently intracellular glutathione content was increased [Sugiyama, Izawa and Inoue (2000) J. Biol. Chem. 275, 15535-15540]. In the present study, we discuss the physiological role of glutathione in the heat-shock stress response in this yeast. Both gsh1 and gsh2 mutants could acquire thermotolerance by mild heat-shock stress and induction of Hsp104p in both mutants was normal; however, mutant cells died faster by heat shock than their parental wild-type strain. After pretreatment at a sublethal temperature, the number of respiration-deficient mutants increased in a gsh1 mutant strain in the early stages of exposure to a lethal temperature, although this increase was partially suppressed by the addition of glutathione. These results lead us to suspect that an increase of glutathione synthesis during heat-shock stress is to protect mitochondrial DNA from oxidative damage. To investigate the correlation between mitochondrial DNA damage and glutathione, mitochondrial Mn-superoxide dismutase (the SOD2 gene product) was disrupted. As a result, the rate of generation of respiration-deficient mutants of a sod2 delta strain was higher than that of the isogenic wild-type strain and treatment of the sod2 delta mutant with buthionine sulphoximine, an inhibitor of glutathione synthesis, inhibited cell growth. These results suggest that glutathione synthesis is induced by heat shock to protect the mitochondrial DNA from oxidative damage that may lead to cell death. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/11062059/Role_of_glutathione_in_heat_shock_induced_cell_death_of_Saccharomyces_cerevisiae_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/11062059/ DB - PRIME DP - Unbound Medicine ER -